US5352574A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Surface functionalized and derivatized electroactive polymers with immobilized active moieties
Assignee: Individual
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Filing: 1991-10-04
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Granted: 1994-10-04
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Expires: 2011-10-04
Abstract
Abstract New variants of electroactive and optoactive polymers are formed from the surface chemical modification and derivization of free-standing and substrate-supported polymer films. The free-standing or substrate-supported films are chemically modified at or near their surfaces to introduce hydrophilic and/or reactive functional groups, such as carboxylic acids, hydroxyls, and amines. Surface derivatization of the modified polymer film is achieved through the specific attachment of bioactive, immunoactive, electroactive, and catalytic agents to the surface of the electroactive or optoactive polymer film. In one embodiment, a polymer selected from polyacetylene, polypyrrole, polyanilane and polythiophene is modified to contain functional groups and an indicator reagent is covalently coupled to the functional groups. When an analyte in a sample reacts with the indicator reagent, electrical conductivity of the polymer is changed and presence of the analyte is indicated by the change in electrical conductivity.
Inventors
- 👤 Anthony Guiseppi-Elie
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 04:12
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/02; G01N27/04; G01N27/12; G01N27/125; G01N27/126; C; C08; C08F; C08F8/00; C08F8/12; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; C12Q1/003; C12Q1/005; B; B01; B01J; B01J2219/00; B01J2219/00274; B01J2219/00583; B01J2219/00603; B01J2219/00653; B01J2219/00659; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
136 words
A process for the immobilization of an indicator reagent upon a chemically modified surface of an electroactive polymeric substrate having a predetermined electrical conductivity, the process comprising: (a) reacting an electroactive polymeric substrate selected from the group consisting of polyacetylene, polypyrrole, polyaniline and polythiophene with at least one chemical modification reagent to produce an activated surface containing new functional groups capable of covalent bonding on the surface of the substrate, the activated surface being associated with a predetermined electrical conductivity; (b) reacting the activated surface on the substrate with a linking agent; and (c) reacting the linking agent with an indicator reagent to covalently band the indicator reagent to the activated surface of the substrate such that the predetermined electrical conductivity of the substrate can be altered by reactions of an analyte with the indicator reagent.
Claim 2
Dependent Claim
136 words
A process for the immobilization of an indicator reagent upon a chemically modified surface of an electroactive polymeric substrate having a predetermined electrical conductivity, the process comprising: (a) reacting an electroactive polymeric substrate selected from the group consisting of polyacetylene, polypyrrole, polyaniline and polythiophene with at least one chemical modification reagent to produce an activated surface containing new functional groups capable of covalent bonding on the surface of the substrate, the activated surface being associated with a predetermined electrical conductivity; (b) reacting the activated surface on the substrate with a linking agent; and (c) reacting the linking agent with an indicator reagent to covalently band the indicator reagent to the activated surface of the substrate such that the predetermined electrical conductivity of the substrate can be altered by reactions of an analyte with the indicator reagent.
Claim 3
Dependent Claim
24 words
The process of claim 1 wherein the electroactive polymer is polyacetylene and the method of surface activation comprises the formation of surface hydroxyl groups.
Claim 4
Dependent Claim
17 words
The process of claim 1 wherein the electroactive polymer is polyacetylene and the linking agent is p-benzoquinone.
Claim 5
Dependent Claim
12 words
The process of claim 3 wherein the indicator reagent is glucose oxidase.
Claim 6
Dependent Claim
35 words
The process of claim 1 wherein the electroactive polymer is polyanaline and the method of surface activation comprises immersion of the polymer in a mixed sulphuric:nitric acid solution and said activated surface comprises amino groups.
Claim 7
Dependent Claim
16 words
The process of claim 1 wherein the electroactive polymer polyanaline and the linking agent is carbodiimide.
Claim 8
Dependent Claim
13 words
The process of claim 6 wherein the indicator reagent is flavin adenine dinucleotide.
Claim 9
Dependent Claim
17 words
The process of claim 1 wherein the electroactive polymer is polyanaline and the linking agent is p-benzoquinone.
Claim 10
Dependent Claim
11 words
The process of claim 8 wherein the indicator reagent glucose oxidase.
Claim 11
Independent Claim
149 words
A method for detecting the presence of an analyte in a sample comprising contacting a sample with a material comprising an electroactive polymeric substrate having a predetermined electrical conductivity containing a covalently bound indicator reagent, reacting analyte in the sample with the indicator reagent to change the electrical conductivity of the suffererate and from the change in the electrical conductivity determining the presence of the analyte, said material being prepared by reacting an electroactive polymeric substrate selected from the group consisting of polyacetylene, polypyrrole, polyaniline and polythiophene having a predetermined electrical conductivity at least one chemical modification reagent to produce an activated surface containing new functional groups capable of covalent bonding on the surface of the substrate and having a predetermined electrical conductivity, and reacting the new functional groups of the activated surface with an indicator reagent to covalently bond the indicator reagent to the surface of the substrate.
Claim 12
Independent Claim
149 words
A method for detecting the presence of an analyte in a sample comprising contacting a sample with a material comprising an electroactive polymeric substrate having a predetermined electrical conductivity containing a covalently bound indicator reagent, reacting analyte in the sample with the indicator reagent to change the electrical conductivity of the suffererate and from the change in the electrical conductivity determining the presence of the analyte, said material being prepared by reacting an electroactive polymeric substrate selected from the group consisting of polyacetylene, polypyrrole, polyaniline and polythiophene having a predetermined electrical conductivity at least one chemical modification reagent to produce an activated surface containing new functional groups capable of covalent bonding on the surface of the substrate and having a predetermined electrical conductivity, and reacting the new functional groups of the activated surface with an indicator reagent to covalently bond the indicator reagent to the surface of the substrate.
Claim 13
Dependent Claim
127 words
A material comprising; an electroactive polymeric substrate containing a covalently bound indicator reagent, said material being prepared by reacting an electroactive polymeric substrate selected from the group consisting of polyacetylene, polypyrrole, polyaniline and polythiophene having a predetermined electrical conductivity with at least one chemical modification reagent to produce an activated surface on the substrate containing new functional groups capable of covalent bonding on the surface of said substrate and said activated surface having a predetermined electrical conductivity, reacting said activated surface with a linking agent, and reacting the linking agent with an indicator reagent to covalently bond the indicator reagent to the activated surface of said substrate, whereby the predetermined electrical conductivity of the substrate can be altered by reactions of an analyte with the indicator reagent.
Claim 14
Dependent Claim
127 words
A material comprising; an electroactive polymeric substrate containing a covalently bound indicator reagent, said material being prepared by reacting an electroactive polymeric substrate selected from the group consisting of polyacetylene, polypyrrole, polyaniline and polythiophene having a predetermined electrical conductivity with at least one chemical modification reagent to produce an activated surface on the substrate containing new functional groups capable of covalent bonding on the surface of said substrate and said activated surface having a predetermined electrical conductivity, reacting said activated surface with a linking agent, and reacting the linking agent with an indicator reagent to covalently bond the indicator reagent to the activated surface of said substrate, whereby the predetermined electrical conductivity of the substrate can be altered by reactions of an analyte with the indicator reagent.
References Cited (Backward Citations)
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