US5372784A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Measurement of bacterial CO2 production in an isolated fluorophore by monitoring an absorbance regulated change of fluorescence
Assignee: Dade Behring Inc
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Filing: 1994-03-11
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Granted: 1994-12-13
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Expires: 2014-03-11
Abstract
Abstract A multi-layer blood culture sensor includes two matrices. The first matrix is a polymer that is permeable to carbon dioxide and water, but impermeable to protons. A pH sensitive absorbance based dye is encapsulated or isolated in the polymer. The second matrix is a polymer with a pH insensitive fluorescent dye encapsulated or isolated in the polymer. The matrices are spectrally coupled and are useful for the determination of microorganisms in a blood culture bottle.
Inventors
- 👤 Roger J. Morris
- 👤 Shoshana Bascomb
- 👤 Carolyn S. Olson
- 👤 Jamie Bobolis
- 👤 David Sherman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 20:56
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/75; G01N21/77; G01N21/78; G01N21/80; C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/06; G01N21/17; G01N21/25; G01N21/27; G01N21/272; G01N21/47; G01N21/49; G01N21/53; G01N21/534; G01N2201/00; G01N2201/12; G01N2201/122; Y; Y10; Y10S; Y10S435/00; Y10S435/808
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
64 words
A multi-layer sensor for determining the concentration or presence of a microorganism in a bodily fluid which comprises: a. a pH sensitive absorbance based dye encapsulated in a first light transmissive, gas permeable, proton impermeable matrix; and b. a pH insensitive fluorescence dye encapsulated in an inert light transparent second matrix, wherein said first and second matrices are spectrally coupled and in close proximity.
Claim 2
Dependent Claim
64 words
A multi-layer sensor for determining the concentration or presence of a microorganism in a bodily fluid which comprises: a. a pH sensitive absorbance based dye encapsulated in a first light transmissive, gas permeable, proton impermeable matrix; and b. a pH insensitive fluorescence dye encapsulated in an inert light transparent second matrix, wherein said first and second matrices are spectrally coupled and in close proximity.
Claim 3
Dependent Claim
25 words
The multi-layer sensor of claim 1 wherein said pH sensitive absorbance based dye is selected from the group consisting of xylenol blue and bromothymol blue.
Claim 4
Dependent Claim
22 words
The multi-layer sensor of claim 1 wherein said fluorescence dye is selected from the group consisting of rhodamine 101 and rhodamine B.
Claim 5
Dependent Claim
22 words
The multi-layer sensor of claim 1 wherein said first and second matrices are selected from the group consisting of silicone and acrylic.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-12-12 | 1998-06-13 | 1998-12-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-12-12 | 2002-06-13 | 2002-12-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-12-12 | 2006-06-13 | 2006-12-13 | $7700.00 | Window Closed / Lapsed |