US5378601A
Expired / Lapsed
US
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Method of preserving platelets with apyrase and an antioxidant
Assignee: Montefiore Medical Center
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Filing: 1992-07-24
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Granted: 1995-01-03
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Expires: 2012-07-24
Abstract
Abstract The invention provides a method and composition for preserving platelets which is based on the use of a composition which includes apyrase and an antioxidant. Preferred compositions will also include a protease inhibitor and optionally a solvent.
Inventors
- 👤 Elena Gepner-Puszkin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 16:42
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/16; A61K38/00; A61K38/16; A61K38/55
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
79 words
A method for the preservation of human blood platelets which consists essentially of: (a) adding an effective amount of an adenosine-5'-triphosphatase and an adenosine-5'-diphosphatase enzyme or an enzyme having activity of both adenosine-5'-triphosphatase and adenosine-5'-diphosphatase. which does not adversely affect platelets in a diluent, to platelets to form a mixture; (b) adding an antioxidant to the mixture of step(a); and (c) maintaining the mixture of step (b) at a temperature which does not affect the viability of the platelets.
Claim 2
Independent Claim
79 words
A method for the preservation of human blood platelets which consists essentially of: (a) adding an effective amount of an adenosine-5'-triphosphatase and an adenosine-5'-diphosphatase enzyme or an enzyme having activity of both adenosine-5'-triphosphatase and adenosine-5'-diphosphatase. which does not adversely affect platelets in a diluent, to platelets to form a mixture; (b) adding an antioxidant to the mixture of step(a); and (c) maintaining the mixture of step (b) at a temperature which does not affect the viability of the platelets.
Claim 3
Dependent Claim
20 words
A method for the preservation of human blood platelets as defined in claim 1 wherein the antioxidant is ascorbic acid.
Claim 4
Dependent Claim
23 words
A method for the preservation of human blood platelets as defined in claim 1 wherein a protease inhibitor is added to step (a).
Claim 5
Dependent Claim
21 words
A method for the preservation of human blood platelets as defined in claim 1 wherein the adenosine-5'-triphosphatase and adenosine-5'-diphosphatase is apyrase.
Claim 6
Dependent Claim
23 words
A method for the preservation of human blood platelets as defined in claim 1 wherein the temperature is between 20° to 24° C.
Claim 7
Dependent Claim
23 words
A method for the preservation of human blood platelets as defined in claim 1 wherein the temperature is between -70° to -195° C.
Claim 8
Independent Claim
119 words
A method for the preservation of human blood platelets which consists essentially of the steps of: (a) obtaining platelets from a donor; (b) concentrating the platelets; (c) adding from 0.07 to 0.15 mg of apyrase per ml of platelets, from 1.2 to 1.5 Thrombin Inhibitor Units of aprotinin and 5-15 mM of ascorbic acid dissolved in saline or in plasma to the platelets of step (b) to form a platelet suspension; (d) adding to the platelet suspension of step (c) a mixture of autologous plasma and dimethyl sulfoxide in a sufficient amount so that the final concentration is 5% dimethyl sulfoxide; and (e) maintaining the product of step (d) at a temperature of about -70° C. to -195° C.
Claim 9
Independent Claim
119 words
A method for the preservation of human blood platelets which consists essentially of the steps of: (a) obtaining platelets from a donor; (b) concentrating the platelets; (c) adding from 0.07 to 0.15 mg of apyrase per ml of platelets, from 1.2 to 1.5 Thrombin Inhibitor Units of aprotinin and 5-15 mM of ascorbic acid dissolved in saline or in plasma to the platelets of step (b) to form a platelet suspension; (d) adding to the platelet suspension of step (c) a mixture of autologous plasma and dimethyl sulfoxide in a sufficient amount so that the final concentration is 5% dimethyl sulfoxide; and (e) maintaining the product of step (d) at a temperature of about -70° C. to -195° C.
Claim 10
Dependent Claim
47 words
A preserved platelet concentrate which comprises: (a) a platelet concentrate; (b) an amount of apyrase which is effective for platelet preservation; (c) an amount of an antioxidant which is effective for platelet preservation; and (d) an amount of a protease inhibitor which is effective for platelet preservation.
Claim 11
Dependent Claim
47 words
A preserved platelet concentrate which comprises: (a) a platelet concentrate; (b) an amount of apyrase which is effective for platelet preservation; (c) an amount of an antioxidant which is effective for platelet preservation; and (d) an amount of a protease inhibitor which is effective for platelet preservation.
Claim 12
Dependent Claim
23 words
A preserved platelet concentrate as defined in claim 8 wherein the antioxidant is ascorbic acid and dimethyl sulfoxide is added as a diluent.
Claim 13
Dependent Claim
15 words
A preserved platelet concentrate as defined in claim 9 wherein the protease inhibitor is aprotinin.
Claim 14
Dependent Claim
14 words
A preserved platelet concentrate as defined in claim 9 which also includes a solvent.
Claim 15
Dependent Claim
26 words
A composition for preserving platelets which consists essentially of: (a) an effective amount of apyrase; (b) an effective amount of an antioxidant; and (c) a diluent
Claim 16
Dependent Claim
26 words
A composition for preserving platelets which consists essentially of: (a) an effective amount of apyrase; (b) an effective amount of an antioxidant; and (c) a diluent
Claim 17
Dependent Claim
16 words
A composition for preserving platelets as defined in claim 12 which also includes a protease inhibitor.
Claim 18
Dependent Claim
15 words
A preserved platelet concentrate as defined in claim 13 which includes the solvent dimethyl sulfoxide.
Claim 19
Dependent Claim
13 words
A composition as defined in claim 13 wherein the protease inhibitor is aprotinin.
References Cited (Backward Citations)
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