Apparatus for detecting fluorescence of particles in a fluid and analyzing the particles

Claim 1

An apparatus for analyzing particles by discharging a sample liquid containing particles to be detected from a nozzle of a flow cell, passing a sheath liquid around the sample liquid to form a sheath flow, emitting light to the sample liquid flow, detecting the light from the particles, and analyzing the particles according to detection signals associated with the detected light, comprising: said flow cell for forming said sample liquid flow as a flat flow, thin in thickness and broad in width; a light source for said emitting light emitted to said sample liquid; an image intensifier for intensifying the fluorescence image of the particles excited by the light and emitted from the broad side of said sample liquid flow on an input plane of said image intensifier; a one-dimensional image sensor extended at an output plane side of said image intensifier in a vertical direction to the flow direction of particles, for scanning a fluorescence image amplified by said image intensifier, and issuing an imaging signal Sf (i) at every scanning; and signal processing means for performing at least one of processing signals and operating on the imaging signals SF (i) from thr one-dimensional image sensor.

Claim 2

An apparatus for analyzing particles by discharging a sample liquid containing particles to be detected from a nozzle of a flow cell, passing a sheath liquid around the sample liquid to form a sheath flow, emitting light to the sample liquid flow, detecting the light from the particles, and analyzing the particles according to detection signals associated with the detected light, comprising: said flow cell for forming said sample liquid flow as a flat flow, thin in thickness and broad in width; a light source for said emitting light emitted to said sample liquid; an image intensifier for intensifying the fluorescence image of the particles excited by the light and emitted from the broad side of said sample liquid flow on an input plane of said image intensifier; a one-dimensional image sensor extended at an output plane side of said image intensifier in a vertical direction to the flow direction of particles, for scanning a fluorescence image amplified by said image intensifier, and issuing an imaging signal Sf (i) at every scanning; and signal processing means for performing at least one of processing signals and operating on the imaging signals SF (i) from thr one-dimensional image sensor.

Claim 5

An apparatus for analyzing particles by discharging a sample liquid containing particles to be detected from a nozzle of a flow cell, passing a sheath liquid around the sample liquid to form a sheath flow, emitting light to the sample liquid flow, detecting the light from the particles, and analyzing the particles according to detection signals associated with the detected light, comprising: said flow cell for forming said sample liquid flow as a flat flow, thin in thickness and broad in width; a first light source for emitting first light to said sample liquid; an image intensifier for intensifying the fluorescence image of the particles excited by the light emitted by said first light source and emitted from the broad side of said sample liquid flow on an input plane of said image intensifier; a first one-dimensional image sensor extended at the output plane side of said image intensifier for scanning a fluorescence image amplified by said image intensifier and issuing an imaging signal Sf (i) in every scanning i; a second light source for emitting second light different in wavelength from the first light to said sample liquid; a second one-dimensional image sensor extended in a vertical direction to the particle flow direction for scanning the particle transmitted light image of the broader side of said sample liquid flow and issuing an imaging signal St (i) in every scanning i; and signal processing means for performing at least one of processing signals and operating on the basis of the imaging signal Sf (i) from said first one-dimensional image sensor and the imaging signal St (i) from said second one-dimensional image sensor.

Claim 6

An apparatus for analyzing particles by discharging a sample liquid containing particles to be detected from a nozzle of a flow cell, passing a sheath liquid around the sample liquid to form a sheath flow, emitting light to the sample liquid flow, detecting the light from the particles, and analyzing the particles according to detection signals associated with the detected light, comprising: said flow cell for forming said sample liquid flow as a flat flow, thin in thickness and broad in width; a first light source for emitting first light to said sample liquid; an image intensifier for intensifying the fluorescence image of the particles excited by the light emitted by said first light source and emitted from the broad side of said sample liquid flow on an input plane of said image intensifier; a first one-dimensional image sensor extended at the output plane side of said image intensifier for scanning a fluorescence image amplified by said image intensifier and issuing an imaging signal Sf (i) in every scanning i; a second light source for emitting second light different in wavelength from the first light to said sample liquid; a second one-dimensional image sensor extended in a vertical direction to the particle flow direction for scanning the particle transmitted light image of the broader side of said sample liquid flow and issuing an imaging signal St (i) in every scanning i; and signal processing means for performing at least one of processing signals and operating on the basis of the imaging signal Sf (i) from said first one-dimensional image sensor and the imaging signal St (i) from said second one-dimensional image sensor.