Patent US5478751A successfully refreshed from Google Patents.
US5478751A
Expired / Lapsed
US
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Self-venting immunodiagnositic devices and methods of performing assays
Assignee: Abbott Laboratories
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Filing: 1994-04-18
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Granted: 1995-12-26
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Expires: 2014-04-18
Abstract
Abstract Methods and devices are provided involving an inlet port, at least one chamber, a channel providing access for fluids to flow through via capillary action or differential pressure, reagents, detection means and self-venting materials. The devices allow for the appropriate mixing, reacting, incubating needed to give a detectable signal which can be read. The self-venting materials allow for the 1) displacement of gases inside a track to the outside of the device and 2) oxygen movement into the track from the outside.
Inventors
- 👤 Gary M. Oosta
- 👤 Thomas G. Schapira
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 01:39
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; B01L3/5027; B01L3/502723; B01L2200/00; B01L2200/06; B01L2200/0684; B01L2300/00; B01L2300/08; B01L2300/0809; B01L2300/0825; B01L2300/0887; B01L2400/00; B01L2400/04; B01L2400/0403; B01L2400/0406; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25375; Y10T436/255
Claims Analysis
Total Claims: 50
Claim 1
Independent Claim
186 words
A method for detecting the presence or an amount of an analyte in a test sample, comprising providing: (a) an analytical device comprising: a housing made of a hydrophobic material, said hydrophobic material consisting of: acrylics, polycarbonates, polystyrenes, silicones, polyurethanes, polyolefins, polytetrafluoroethylenes, polypropylenes, polyethylenes, thermoplastic elastomers, copolymers, acrylnitrylbutadienestyrene, and styreneacrylonitrile; said housing containing an inlet port, said inlet port accessing a track of predetermined width and length within said housing and having at least one reagent therein; said track having at least one hydrophobic surface modified to create a hydrophilic surface by introducing at least one hydrophilic group onto said hydrophobic surface, said hydrophilic group consisting of: hydroxyls, carbonyls, carboxylics, aminos, sulfonics, sulfonates, sulfates, pyrroles, acetates, acrylics, carbonates, amidos, and phosphates; said hydrophobic material being impermeable to said test sample and allowing gaseous exchange in and out of a portion of said track; (b) adding said test sample to said housing through said inlet port; said test sample and said reagent producing a detectable signal upon mixing; and (c) determining the presence or an amount of an analyte in said test sample from said detectable signal.
Claim 2
Independent Claim
186 words
A method for detecting the presence or an amount of an analyte in a test sample, comprising providing: (a) an analytical device comprising: a housing made of a hydrophobic material, said hydrophobic material consisting of: acrylics, polycarbonates, polystyrenes, silicones, polyurethanes, polyolefins, polytetrafluoroethylenes, polypropylenes, polyethylenes, thermoplastic elastomers, copolymers, acrylnitrylbutadienestyrene, and styreneacrylonitrile; said housing containing an inlet port, said inlet port accessing a track of predetermined width and length within said housing and having at least one reagent therein; said track having at least one hydrophobic surface modified to create a hydrophilic surface by introducing at least one hydrophilic group onto said hydrophobic surface, said hydrophilic group consisting of: hydroxyls, carbonyls, carboxylics, aminos, sulfonics, sulfonates, sulfates, pyrroles, acetates, acrylics, carbonates, amidos, and phosphates; said hydrophobic material being impermeable to said test sample and allowing gaseous exchange in and out of a portion of said track; (b) adding said test sample to said housing through said inlet port; said test sample and said reagent producing a detectable signal upon mixing; and (c) determining the presence or an amount of an analyte in said test sample from said detectable signal.
Claim 3
Dependent Claim
58 words
The method of claim 1 wherein said analyte is a member of a group consisting of: proteins, peptides, amino acids, carbohydrates, hormones, steroids, vitamins, lipids, nucleic acids, trace elements, drugs including those administered for therapeutic purposes as well as those administered for illicit purposes, bacteria, viruses, metabolites, viroids, mammalian cells such as lymphocytes, epithelial cells, and neoplastic cells.
Claim 4
Dependent Claim
16 words
The method of claim 1 wherein said detectable signal is read directly from said analytical device.
Claim 5
Dependent Claim
19 words
The method of claim 1 wherein said detectable signal is read directly from said analytical device by an instrument.
Claim 6
Dependent Claim
25 words
The method of claim 4 wherein said instrument is a member of a group consisting of: spectrophotometers, colorimeters, fluorimeters, spectroscopies, calorimeters, reflectance meters, and conductimeters.
Claim 7
Dependent Claim
28 words
The method of claim 1 wherein said modification of said hydrophobic surface is by wet chemical modification, surface coatings, gas modification, plasma deposition, plasma modification treatments and sufactants.
Claim 8
Independent Claim
189 words
A method for detecting the presence or an amount of an analyte in a test sample comprising providing: (a) an analytical device comprising a first hydrophobic material and a second hydrophilic material, said first hydrophobic material consisting of: acrylics, polycarbonates, polystyrenes, silicones, polyurethanes, polyolefins, polytetrafluoroethylenes, polypropylenes, polyethylenes thermoplastic elastomers, copolymers, acrylnitrylbutadienestyrene, and styreneacrylonitrile; said housing containing an inlet port, said inlet port accessing a track of predetermined width and length within said housing and containing a reagent therein; said first hydrophobic material allowing gaseous exchange in and out of a portion of said track; and said track having at least one surface of said first hydrphobic material modified to create said second hydrophilic material by introducing at least one hydrophilic group onto said first hydrophobic surface, said hydrophilic group consisting of: hydroxyls, carbonyls, carboxylics, aminos, sulfonics, sulfonates, sulfates, pyrroles, acetates, acrylics, carbonates, amidos, and phosphates; and (b) adding said test sample to said housing through said inlet port; said test sample and said reagent producing a detectable signal upon mixing; and (c) determining the presence or an amount of an analyte in said test sample from said detectable signal.
Claim 9
Independent Claim
189 words
A method for detecting the presence or an amount of an analyte in a test sample comprising providing: (a) an analytical device comprising a first hydrophobic material and a second hydrophilic material, said first hydrophobic material consisting of: acrylics, polycarbonates, polystyrenes, silicones, polyurethanes, polyolefins, polytetrafluoroethylenes, polypropylenes, polyethylenes thermoplastic elastomers, copolymers, acrylnitrylbutadienestyrene, and styreneacrylonitrile; said housing containing an inlet port, said inlet port accessing a track of predetermined width and length within said housing and containing a reagent therein; said first hydrophobic material allowing gaseous exchange in and out of a portion of said track; and said track having at least one surface of said first hydrphobic material modified to create said second hydrophilic material by introducing at least one hydrophilic group onto said first hydrophobic surface, said hydrophilic group consisting of: hydroxyls, carbonyls, carboxylics, aminos, sulfonics, sulfonates, sulfates, pyrroles, acetates, acrylics, carbonates, amidos, and phosphates; and (b) adding said test sample to said housing through said inlet port; said test sample and said reagent producing a detectable signal upon mixing; and (c) determining the presence or an amount of an analyte in said test sample from said detectable signal.
Claim 10
Dependent Claim
58 words
The method of claim 7 wherein said analyte is a member of a group consisting of: proteins, peptides, amino acids, carbohydrates, hormones, steroids, vitamins, lipids, nucleic acids, trace elements, drugs including those administered for therapeutic purposes as well as those administered for illicit purposes, bacteria, viruses, metabolites, viroids, mammalian cells such as lymphocytes, epithelial cells, and neoplastic cells.
Claim 11
Dependent Claim
16 words
The method of claim 7 wherein said detectable signal is read directly from said analytical device.
Claim 12
Dependent Claim
19 words
The method of claim 7 wherein said detectable signal is read directly from said analytical device by an instrument.
Claim 13
Dependent Claim
25 words
The method of claim 10 wherein said instrument is a member of a group consisting of: spectrophotometers, colorimeters, fluorimeters, spectroscopies, calorimeters, reflectance meters, and conductimeters.
Claim 14
Independent Claim
178 words
A method for detecting the presence or an amount of an analyte in a test sample comprising providing: (a) a housing having a first layer, a core layer, and a second layer, said core layer made of a hydrophobic material containing a track of predetermined width and length and having at least one reagent therein, said track having a sidewall defining the boundaries for flow of said test sample, said first and second layers being impermeable to said test sample; at least one of said first or second layers having a hydrophilic surface; said housing containing an inlet port, said inlet port accessing said track, at least one of said first layer, second layer, or core layer made of a porous material that will vent gases in and out of a portion of said track; (b) adding said test sample to said housing through said inlet port; said test sample and said reagent producing a detectable signal upon mixing; and (c) determining the presence or an amount of an analyte in said test sample from said detectable signal.
Claim 15
Independent Claim
178 words
A method for detecting the presence or an amount of an analyte in a test sample comprising providing: (a) a housing having a first layer, a core layer, and a second layer, said core layer made of a hydrophobic material containing a track of predetermined width and length and having at least one reagent therein, said track having a sidewall defining the boundaries for flow of said test sample, said first and second layers being impermeable to said test sample; at least one of said first or second layers having a hydrophilic surface; said housing containing an inlet port, said inlet port accessing said track, at least one of said first layer, second layer, or core layer made of a porous material that will vent gases in and out of a portion of said track; (b) adding said test sample to said housing through said inlet port; said test sample and said reagent producing a detectable signal upon mixing; and (c) determining the presence or an amount of an analyte in said test sample from said detectable signal.
Claim 16
Dependent Claim
58 words
The method of claim 12 wherein said analyte is a member of a group consisting of: proteins, peptides, amino acids, carbohydrates, hormones, steroids, vitamins, lipids, nucleic acids, trace elements, drugs including those administered for therapeutic purposes as well as those administered for illicit purposes, bacteria, viruses, metabolites, viroids, mammalian cells such as lymphocytes, epithelial cells, and neoplastic cells.
Claim 17
Dependent Claim
16 words
The method of claim 12 wherein said detectable signal is read directly from said analytical device.
Claim 18
Dependent Claim
19 words
The method of claim 12 wherein said detectable signal is read directly from said analytical device by an instrument.
Claim 19
Dependent Claim
25 words
The method of claim 15 wherein said instrument is a member of a group consisting of: spectrophotometers, colorimeters, fluorimeters, spectroscopies, calorimeters, reflectance meters, and conductimeters.
Claim 20
Dependent Claim
131 words
An analytical device for detecting the presence or an amount of an analyte in a test sample, comprising: a housing made of a hydrophobic material, said hydrophobic material consisting of: acrylics, polycarbonates, polystyrenes, silicones, polyurethanes, polyolefins, polytetrafluoroethylenes, polypropylenes, polyethylenes, thermoplastic elastomers, copolymers, acrylnitrylbutadienestyrene, and styreneacrylonitrile; said housing containing an inlet port, said inlet port accessing a track of predetermined width and length within housing; said track having at least one hydrophobic surface modified to create a hydrophilic surface by introducing at least one hydrophilic group onto said hydrophobic surface, said hydrophilic group consisting of: hydroxyls, carbonyls, carboxylics, aminos, sulfonics, sulfonates, sulfates, pyrroles, acetates, acrylics, carbonates, amidos, and phosphates; and said hydrophobic material being impermeable to said test sample and allowing gaseous exchange in and out of a portion of said track.
Claim 21
Dependent Claim
131 words
An analytical device for detecting the presence or an amount of an analyte in a test sample, comprising: a housing made of a hydrophobic material, said hydrophobic material consisting of: acrylics, polycarbonates, polystyrenes, silicones, polyurethanes, polyolefins, polytetrafluoroethylenes, polypropylenes, polyethylenes, thermoplastic elastomers, copolymers, acrylnitrylbutadienestyrene, and styreneacrylonitrile; said housing containing an inlet port, said inlet port accessing a track of predetermined width and length within housing; said track having at least one hydrophobic surface modified to create a hydrophilic surface by introducing at least one hydrophilic group onto said hydrophobic surface, said hydrophilic group consisting of: hydroxyls, carbonyls, carboxylics, aminos, sulfonics, sulfonates, sulfates, pyrroles, acetates, acrylics, carbonates, amidos, and phosphates; and said hydrophobic material being impermeable to said test sample and allowing gaseous exchange in and out of a portion of said track.
Claim 22
Dependent Claim
14 words
The analytical device of claim 17 wherein said track has at least one chamber.
Claim 23
Dependent Claim
13 words
The analytical device of claim 17 further comprising a reagent within said device.
Claim 24
Dependent Claim
14 words
The analytical device of claim 19 wherein said reagent is on said hydrophilic surface.
Claim 25
Dependent Claim
18 words
The analytical device of claim 17 wherein said test sample flows along said hydrophilic surface by differential pressure.
Claim 26
Dependent Claim
12 words
The analytical device of claim 17 wherein said device is a cuvette.
Claim 27
Dependent Claim
30 words
The analytical device of claim 17 wherein said modification of said hydrophobic surface is by wet chemical modification, surface coatings, gas modification, plasma deposition, plasma modification treatments, and a surfactant.
Claim 28
Dependent Claim
139 words
An analytical device for detecting the presence or an amount of an analyte in a test sample comprising: a housing made of a first hydrophobic material and a second hydrophilic material, said first hydrophobic material consisting of: acrylics, polycarbonates, polystyrenes, silicones, polyurethanes, polyolefins, polytetrafluoroethylenes, polypropylenes, polyethylenes, thermoplastic elastomers, copolymers, acrylnitrylbutadienestyrene, and styreneacrylonitrile; said housing containing an inlet port, said inlet port accessing a track of predetermined width and length within said housing; said first hydrophobic material allowing gaseous exchange in and out of a portion of said track; and said track having at least one surface of said first hydrophobic material modified to create said second hydrophilic material by introducing at least one hydrophilic group onto said first hydrophobic surface, said hydrophilic group consisting of: hydroxyls, carbonyls, carboxylics, aminos, sulfonics, sulfonates, sulfates, pyrroles, acetates, acrylics, carbonates, amidos, and phosphates.
Claim 29
Dependent Claim
139 words
An analytical device for detecting the presence or an amount of an analyte in a test sample comprising: a housing made of a first hydrophobic material and a second hydrophilic material, said first hydrophobic material consisting of: acrylics, polycarbonates, polystyrenes, silicones, polyurethanes, polyolefins, polytetrafluoroethylenes, polypropylenes, polyethylenes, thermoplastic elastomers, copolymers, acrylnitrylbutadienestyrene, and styreneacrylonitrile; said housing containing an inlet port, said inlet port accessing a track of predetermined width and length within said housing; said first hydrophobic material allowing gaseous exchange in and out of a portion of said track; and said track having at least one surface of said first hydrophobic material modified to create said second hydrophilic material by introducing at least one hydrophilic group onto said first hydrophobic surface, said hydrophilic group consisting of: hydroxyls, carbonyls, carboxylics, aminos, sulfonics, sulfonates, sulfates, pyrroles, acetates, acrylics, carbonates, amidos, and phosphates.
Claim 30
Dependent Claim
14 words
The analytical device of claim 24 wherein said track has at least one chamber.
Claim 31
Dependent Claim
17 words
The analytical device of claim 24 wherein said reagent is on the surface of said hydrophilic surface.
Claim 32
Dependent Claim
17 words
The analytical device of claim 24 wherein said reagent is on the hydrophobic surface of said track.
Claim 33
Dependent Claim
20 words
The analytical device of claim 24 wherein said test sample is moved along said hydrophilic surface by a differential pressure.
Claim 34
Dependent Claim
12 words
The analytical device of claim 24 wherein said device is a cuvette.
Claim 35
Dependent Claim
30 words
The analytical device of claim 24 wherein said modification of said first hydrophobic surface is by wet chemical modification, surface coatings, gas modification, plasma deposition, plasma modification treatments, and surfactants.
Claim 36
Dependent Claim
22 words
The analytical device of claim 24 wherein said hydrophilic material is modified by application of an adhesive system to a polymer screen.
Claim 37
Dependent Claim
36 words
The analytical device of claim 24 wherein said hydrophilic material is modified by an adhesive systems consisting of: hot melt adhesives, one part curables, two part curables, solvent based/emulsion adhesives, ultraviolet curables, and water induced curables.
Claim 38
Dependent Claim
23 words
The analytical device of claim 24 wherein said hydrophilic material is modified by application of an adhesive system as one or more islands.
Claim 39
Dependent Claim
22 words
The analytical device of claim 24 wherein said hydrophilic material is modified by application of an adhesive system to a bibulous material.
Claim 40
Dependent Claim
129 words
An analytical device for detecting the presence or an amount of an analyte in a test sample comprising: a housing having a first layer, a core layer, and a second layer, said core layer made of a hydrophobic material containing a track of predetermined width and length, said track having a sidewall defining the boundaries for flow of said test sample, said first and second layers being impermeable to said test sample; at least one of said first or second layers having a hydrophilic surface; and said housing containing an inlet port, said inlet port accessing said track of, at least one of said first layer, second layer, or core layer made of a porous material that will vent gases in and out of a portion of said track.
Claim 41
Dependent Claim
129 words
An analytical device for detecting the presence or an amount of an analyte in a test sample comprising: a housing having a first layer, a core layer, and a second layer, said core layer made of a hydrophobic material containing a track of predetermined width and length, said track having a sidewall defining the boundaries for flow of said test sample, said first and second layers being impermeable to said test sample; at least one of said first or second layers having a hydrophilic surface; and said housing containing an inlet port, said inlet port accessing said track of, at least one of said first layer, second layer, or core layer made of a porous material that will vent gases in and out of a portion of said track.
Claim 42
Dependent Claim
14 words
The analytical device of claim 35 wherein said track has at least one chamber.
Claim 43
Dependent Claim
19 words
The analytical device of claim 35 further comprising a reagent on the surface of said first or second layer.
Claim 44
Dependent Claim
17 words
The analytical device of claim 35 further comprising a reagent on the hydrophobic material of said track.
Claim 45
Dependent Claim
18 words
The analytical device of claim 35 wherein said test sample flows along said hydrophilic surface by differential pressure.
Claim 46
Dependent Claim
12 words
The analytical device of claim 35 wherein said device is a cuvette.
Claim 47
Dependent Claim
27 words
The analytical device of claim 35 wherein said hydrophilic surface is modified by wet chemical modification, surface coatings, gas modification, plasma deposition, plasma modification treatments and surfactants.
Claim 48
Dependent Claim
23 words
The analytical device of claim 35 wherein said core layer is a hydrophilic material which is modified by application of an adhesive system.
Claim 49
Dependent Claim
30 words
The adhesive system of claim 42 selected from the group consisting of: hot melt adhesives, one part curables, two part curables, solvent based/emulsion adhesives, ultraviolet curables, and water induced curables.
Claim 50
Dependent Claim
17 words
The analytical device of claim 42 wherein said adhesive system is applied as one or more islands.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-12-25 | 1999-06-26 | 1999-12-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-12-25 | 2003-06-26 | 2003-12-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-12-25 | 2007-06-26 | 2007-12-26 | $7700.00 | Window Closed / Lapsed |