US5520788A
Expired / Lapsed
US
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Support layer for enzyme electrode laminated membranes
Assignee: YSI Inc
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Filing: 1995-01-17
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Granted: 1996-05-28
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Expires: 2015-01-17
Abstract
Abstract Improved Newman type enzyme containing laminated membranes are disclosed for use in conjunction with polarographic cells to measure analyte concentration in solutions. The laminated membranes comprise a semipermeable outer, support layer including super large pores of greater than about 200 Å in diameter. The outer layer preferably comprises pore sizes of about 380-750 Å in diameter and has a percentage porosity of about 0.005-0.2%.
Inventors
- 👤 Jay M. Johnson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 07:34
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
69 words
Laminated membrane for use in a polarographic cell assembly for assay of an analyte in solution, said laminated membrane comprising: an outer layer having pore sizes of greater than about 200 Angstrom units in diameter and less than about 750 Angstrom units in diameter therein and having a porosity of from about 0.005-0.2%; an inner layer, and an enzyme layer disposed between said outer layer and said inner layer.
Claim 2
Dependent Claim
69 words
Laminated membrane for use in a polarographic cell assembly for assay of an analyte in solution, said laminated membrane comprising: an outer layer having pore sizes of greater than about 200 Angstrom units in diameter and less than about 750 Angstrom units in diameter therein and having a porosity of from about 0.005-0.2%; an inner layer, and an enzyme layer disposed between said outer layer and said inner layer.
Claim 3
Dependent Claim
65 words
Laminated membrane for use in a polarographic cell assembly for assay of an analyte in solution, said laminated membrane comprising: an outer layer having pore sizes on the order of about 380 to 750 Angstrom units in diameter therein and having a porosity of from about 0.005 to 2%; an inner layer, and an enzyme layer disposed between said outer layer and said inner layer.
Claim 4
Dependent Claim
65 words
Laminated membrane for use in a polarographic cell assembly for assay of an analyte in solution, said laminated membrane comprising: an outer layer having pore sizes on the order of about 380 to 750 Angstrom units in diameter therein and having a porosity of from about 0.005 to 2%; an inner layer, and an enzyme layer disposed between said outer layer and said inner layer.
Claim 5
Dependent Claim
25 words
Membrane as recited in claim 2, wherein said outer layer has pore sizes of about 450 Angstrom units and wherein said porosity is about 0.010%.
Claim 6
Dependent Claim
25 words
Membrane as recited in claim 2 wherein said outer layer has pore sizes of about 692 Angstrom units and wherein said porosity is about 0.0225%.
Claim 7
Dependent Claim
25 words
Membrane as recited in claim 2 wherein said outer layer has pore sizes of about 382 Angstrom units and wherein said porosity is about 0.036%.
Claim 8
Dependent Claim
112 words
Laminated membrane for use in a polarographic cell assembly for assay of an analyte selected from the group consisting of glucose and lactate in solution, said assembly comprising a reference electrode and a sensor electrode, said laminated membrane positioned proximate said sensor electrode and comprising: an outer layer of about 5 to 7 microns thick and having pore sizes of greater than about 200 Angstrom units in diameter and less than about 750 Angstroms in diameter and a porosity of from about 0.005-2%; an inner layer of about 2 microns or less in thickness disposed adjacent said sensor electrode, and an enzyme layer disposed between said outer layer and said inner layer.
Claim 9
Dependent Claim
112 words
Laminated membrane for use in a polarographic cell assembly for assay of an analyte selected from the group consisting of glucose and lactate in solution, said assembly comprising a reference electrode and a sensor electrode, said laminated membrane positioned proximate said sensor electrode and comprising: an outer layer of about 5 to 7 microns thick and having pore sizes of greater than about 200 Angstrom units in diameter and less than about 750 Angstroms in diameter and a porosity of from about 0.005-2%; an inner layer of about 2 microns or less in thickness disposed adjacent said sensor electrode, and an enzyme layer disposed between said outer layer and said inner layer.
Claim 10
Dependent Claim
25 words
Membrane as recited in claim 6 wherein said outer layer has pore sizes of about 250 Angstrom units and wherein said porosity is about 0.015%.
Claim 11
Dependent Claim
126 words
Laminated membrane for use in a polarographic cell assembly for assay of an analyte selected from the group consisting of glucose and lactate in solution, said assembly comprising a reference electrode and a sensor electrode, said laminated membrane positioned proximate said sensor electrode and comprising: an outer layer of about 5 to 7 microns thick and having pore sizes of about 380 to 750 Angstrom units in diameter and a porosity of from about 0.005 to 0.2%, said outer layer being composed of a member selected from the group consisting of polycarbonate, regenerated cellulose and polyurethane polymers; an inner layer of about 2 microns or less in thickness disposed adjacent said sensor electrode, and an enzyme layer disposed between said outer layer and said inner layer.
Claim 12
Dependent Claim
126 words
Laminated membrane for use in a polarographic cell assembly for assay of an analyte selected from the group consisting of glucose and lactate in solution, said assembly comprising a reference electrode and a sensor electrode, said laminated membrane positioned proximate said sensor electrode and comprising: an outer layer of about 5 to 7 microns thick and having pore sizes of about 380 to 750 Angstrom units in diameter and a porosity of from about 0.005 to 0.2%, said outer layer being composed of a member selected from the group consisting of polycarbonate, regenerated cellulose and polyurethane polymers; an inner layer of about 2 microns or less in thickness disposed adjacent said sensor electrode, and an enzyme layer disposed between said outer layer and said inner layer.
Claim 13
Dependent Claim
27 words
Membrane as recited in claim 8 wherein said outer layer has pore sizes of about 450 Angstrom units in diameter and wherein said porosity is about 0.010%.
Claim 14
Dependent Claim
27 words
Membrane as recited in claim 8 wherein said outer layer has pore sizes of about 692 Angstrom units in diameter and wherein said porosity is about 0.0225%.
Claim 15
Dependent Claim
27 words
Membrane as recited in claim 8 wherein said outer layer has pore sizes of about 382 Angstrom units in diameter and wherein said porosity is about 0,036%.
Claim 16
Dependent Claim
54 words
Laminated membrane for use in a polarographic cell assembly for assay of an analyte in solution, said laminated membrane comprising: an outer layer comprising a semi-permeable film having pore sizes of about 380-750 Angstrom units in diameter therein; an inner layer; and an enzyme layer disposed between said outer layer and said inner layer.
Claim 17
Dependent Claim
54 words
Laminated membrane for use in a polarographic cell assembly for assay of an analyte in solution, said laminated membrane comprising: an outer layer comprising a semi-permeable film having pore sizes of about 380-750 Angstrom units in diameter therein; an inner layer; and an enzyme layer disposed between said outer layer and said inner layer.
Claim 18
Dependent Claim
21 words
Laminated membrane as recited in claim 12 wherein said outer layer has pore sizes of about 450 Angstrom units in diameter.
Claim 19
Dependent Claim
21 words
Laminated membrane as recited in claim 12 wherein said outer layer has pore sizes of about 692 Angstrom units in diameter.
Claim 20
Dependent Claim
21 words
Laminated membrane as recited in claim 12 wherein said outer layer has pore sizes of about 382 Angstrom units in diameter.
Claim 21
Dependent Claim
19 words
Laminated membrane as recited in claim 12 wherein said outer layer has a porosity percentage of between about 0.005-0.2%.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-05-28 | 1999-11-27 | 2000-05-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-05-28 | 2003-11-27 | 2004-05-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-05-28 | 2007-11-27 | 2008-05-28 | $7700.00 | Window Closed / Lapsed |