Patent US5545895A successfully refreshed from Google Patents.
US5545895A
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Method of standardizing data obtained through mass spectrometry
Assignee: Dow Chemical Co
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Filing: 1995-03-20
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Granted: 1996-08-13
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Expires: 2015-03-20
Abstract
Abstract A method of correcting for instrumental changes that influence the spectral response of a mass spectrometer over time. The method entails determining and applying a transfer function which equalizes instrument bias between mass spectrometers and within the same mass spectrometer over time. A transfer function is specific to a particular mass spectrometer, and is based on the relationship between a reference spectrum of a standard as stored in a library and a spectrum of the standard obtained on the particular mass spectrometer of interest. This method facilitates and simplifies recalibration, provides enhanced library searching capabilities for mass spectrometry, and may permit concentration determination from mass spectral data without the need to recalibrate an instrument with multiple standards.
Inventors
- 👤 Larry G. Wright
- 👤 Mark A. LaPack
- 👤 Wayne W. Blaser
- 👤 Kenneth R. Beebe
- 👤 Mary A. Leugers
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 18:25
CPC Classifications
H; H01; H01J; H01J49/00; H01J49/02; G; G01; G01N; G01N21/00; G01N21/17; G01N21/25; G01N21/27; G01N21/274; G01J; G01J3/00; G01J3/28
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
128 words
A method of determining relative instrument bias from mass spectrometric data, said method comprising the steps of: (a) acquiring a mass spectrometric reference spectrum over a spectral region of interest wherein the reference spectrum is representative of the mass spectrum of a standard, the reference spectrum being referred to as an unbiased reference spectrum, said unbiased reference spectrum being stored and retrievable; (b) acquiring a mass spectrometric working spectrum of an equivalent standard over a corresponding spectral region on a working mass spectrometer, said working spectrum being referred to as a biased working spectrum; and (c) deriving a transfer function from the unbiased reference spectrum and the biased working spectrum, the transfer function comprising an expression which mathematically equates the biased working spectrum to the unbiased reference spectrum.
Claim 2
Independent Claim
128 words
A method of determining relative instrument bias from mass spectrometric data, said method comprising the steps of: (a) acquiring a mass spectrometric reference spectrum over a spectral region of interest wherein the reference spectrum is representative of the mass spectrum of a standard, the reference spectrum being referred to as an unbiased reference spectrum, said unbiased reference spectrum being stored and retrievable; (b) acquiring a mass spectrometric working spectrum of an equivalent standard over a corresponding spectral region on a working mass spectrometer, said working spectrum being referred to as a biased working spectrum; and (c) deriving a transfer function from the unbiased reference spectrum and the biased working spectrum, the transfer function comprising an expression which mathematically equates the biased working spectrum to the unbiased reference spectrum.
Claim 3
Dependent Claim
15 words
The method of claim 1 wherein the transfer function is updated after a time interval.
Claim 4
Dependent Claim
19 words
The method of claim 1 wherein the working spectrum and the reference spectrum are collected on separate mass spectrometers.
Claim 5
Dependent Claim
20 words
The method of claim 1 wherein the working spectrum and the reference spectrum are collected on the working mass spectrometer.
Claim 6
Dependent Claim
105 words
The method of claim 1 wherein an unbiased reference spectrum of a second standard is stored by the steps of: (i) during the same time interval as step (b), acquiring a spectrum of the second standard on the working mass spectrometer over the corresponding spectral region, said spectrum of the second standard being referred to as a biased spectrum of the second standard; (ii) applying the transfer function of step (c) to the biased spectrum of the second standard to obtain an unbiased reference spectrum of the second standard; and (iii) storing at least a portion of the unbiased reference spectrum of the second standard.
Claim 7
Dependent Claim
63 words
The method of claim 1 comprising the further steps of: (i) during the same time interval as step (b), acquiring at least one biased sample spectrum of at least one sample on the working mass spectrometer over a corresponding spectral region; and (ii) applying the transfer function of step (c) to each said sample spectrum to obtain at least one unbiased sample spectrum.
Claim 8
Dependent Claim
29 words
The method of claim 6 wherein the unbiased reference spectrum has peaks at at least every mass-to-charge ratio as that of the sample spectrum in the corresponding spectral region.
Claim 9
Dependent Claim
32 words
The method of claim 1 wherein the standard comprises at least one component such that the standard gives a mass spectrum having peaks at mass-to-charge ratios distributed throughout the corresponding spectral region.
Claim 10
Dependent Claim
26 words
The method of claim 6 wherein said standard comprises a known compound of a known concentration such that the amount of said known concentration is retrievable.
Claim 11
Dependent Claim
56 words
The method of claim 9 further comprising the step of determining the concentration of a single component of interest in a sample by determining the fractional contribution of the unbiased sample spectrum in a spectral region of interest to a corresponding spectral region of the unbiased reference spectrum for a standard containing the component of interest.
Claim 12
Independent Claim
154 words
A method of manipulating mass spectrometric data to determine relative instrument bias, said method comprising the steps of: (a) acquiring a set of mass spectrometric reference spectra over a spectral region of interest from a group of standards wherein the set of reference spectra is representative of the mass spectra of said group of standards, each spectrum in the set of reference spectra being referred to as an unbiased reference spectrum, said unbiased reference spectra being stored and retrievable; (b) acquiring a mass spectrometric working spectrum of an equivalent standard over a corresponding spectral region on a working mass spectrometer, said working spectrum being referred to as a biased working spectrum, said equivalent standard being selected from said group of standards; and (c) deriving a transfer function from the unbiased reference spectrum and the biased working spectrum, the transfer function comprising an expression which mathematically equates the working spectrum to the unbiased reference spectrum.
Claim 13
Independent Claim
154 words
A method of manipulating mass spectrometric data to determine relative instrument bias, said method comprising the steps of: (a) acquiring a set of mass spectrometric reference spectra over a spectral region of interest from a group of standards wherein the set of reference spectra is representative of the mass spectra of said group of standards, each spectrum in the set of reference spectra being referred to as an unbiased reference spectrum, said unbiased reference spectra being stored and retrievable; (b) acquiring a mass spectrometric working spectrum of an equivalent standard over a corresponding spectral region on a working mass spectrometer, said working spectrum being referred to as a biased working spectrum, said equivalent standard being selected from said group of standards; and (c) deriving a transfer function from the unbiased reference spectrum and the biased working spectrum, the transfer function comprising an expression which mathematically equates the working spectrum to the unbiased reference spectrum.
Claim 14
Dependent Claim
15 words
The method of claim 11 wherein the transfer function is updated after a time interval.
Claim 15
Dependent Claim
19 words
The method of claim 11 wherein the working spectrum and the reference spectrum are collected on separate mass spectrometers.
Claim 16
Dependent Claim
20 words
The method of claim 11 wherein the working spectrum and the reference spectrum are collected on the working mass spectrometer.
Claim 17
Dependent Claim
106 words
The method of claim 11 wherein an unbiased reference spectrum of an additional standard may be stored by the steps of: (i) during the same time interval as step (b), acquiring a spectrum of the additional standard on the working mass spectrometer over the corresponding spectral region, said spectrum of the additional standard being referred to as a biased spectrum of the additional standard; (ii) applying the transfer function of step (c) to the biased spectrum of the additional standard to obtain an unbiased reference spectrum of the additional standard; and (iii) storing at least a portion of the unbiased reference spectrum of the additional standard.
Claim 18
Dependent Claim
64 words
The method of claim 11 further comprising the steps of: (i) during the same time interval as step (b), acquiring at least one biased sample spectrum of at least one sample on the working mass spectrometer over a corresponding spectral region; and (ii) applying the transfer function of step (c) to each said biased sample spectrum to obtain at least one unbiased sample spectrum.
Claim 19
Dependent Claim
29 words
The method of claim 16 wherein the unbiased reference spectrum has peaks at at least every mass-to-charge ratio as that of the sample spectrum in the corresponding spectral region.
Claim 20
Dependent Claim
32 words
The method of claim 11 wherein the standard comprises at least one component such that the standard gives a mass spectrum having peaks at mass-to-charge ratios distributed throughout the corresponding spectral region.
Claim 21
Dependent Claim
71 words
The method of claim 16 further comprising the step of determining the concentration of a single component of interest of a sample by relating at least a portion of the unbiased sample spectrum with a corresponding portion of the unbiased reference spectrum for a standard containing the component of interest, wherein said standard comprises a known compound of a known concentration such that the amount of said known concentration is retrievable.
Claim 22
Dependent Claim
80 words
The method of claim 16 further comprising the step of determining the concentration of multiple components of interest by: (a) determining the contribution to said sample spectrum of each said component of interest from the unbiased reference spectra of said components of interest, said stored reference spectra being of known compounds of known concentrations; and (b) relating the contribution of each component of interest to the known concentration of the unbiased reference spectrum corresponding to each said component of interest.
Claim 23
Dependent Claim
37 words
The method of claim 16 further comprising the step of searching the stored unbiased reference spectra for a match with the unbiased sample spectrum to identify the composition of the sample giving rise to said sample spectrum.
Claim 24
Dependent Claim
66 words
The method of claim 21 further comprising the step of determining the concentration of at least one component of interest in said sample by relating information from at least a portion of the unbiased sample spectrum with information from at least a portion of the unbiased reference spectrum for a standard containing the component of interest, said standard being a known compound of a known concentration.
Claim 25
Independent Claim
169 words
A method of standardizing mass spectrometric data, said method comprising the steps of: (a) acquiring a set of mass spectrometric reference spectra from a group of standards wherein the set of reference spectra is representative of the mass spectra of said standards, each spectrum in the set of reference spectra being referred to as an unbiased reference spectrum, said unbiased reference spectra being stored and retrievable; (b) acquiring a mass spectrometric working spectrum of an equivalent standard on a working mass spectrometer, and acquiring a biased sample spectrum of a sample on the working mass spectrometer, said working spectrum being referred to as a biased working spectrum, said equivalent standard being selected from said group of standards; (c) deriving a transfer function from the unbiased reference spectrum and the biased working spectrum, the transfer function comprising an expression which mathematically equates the working spectrum to the unbiased reference spectrum; and (d) applying the transfer function of step (c) to the biased sample spectrum to obtain an unbiased sample spectrum.
Claim 26
Independent Claim
169 words
A method of standardizing mass spectrometric data, said method comprising the steps of: (a) acquiring a set of mass spectrometric reference spectra from a group of standards wherein the set of reference spectra is representative of the mass spectra of said standards, each spectrum in the set of reference spectra being referred to as an unbiased reference spectrum, said unbiased reference spectra being stored and retrievable; (b) acquiring a mass spectrometric working spectrum of an equivalent standard on a working mass spectrometer, and acquiring a biased sample spectrum of a sample on the working mass spectrometer, said working spectrum being referred to as a biased working spectrum, said equivalent standard being selected from said group of standards; (c) deriving a transfer function from the unbiased reference spectrum and the biased working spectrum, the transfer function comprising an expression which mathematically equates the working spectrum to the unbiased reference spectrum; and (d) applying the transfer function of step (c) to the biased sample spectrum to obtain an unbiased sample spectrum.
Claim 27
Dependent Claim
84 words
The method of claim 23 further comprising determining the concentration of a component of interest in the sample by: (i) searching the stored unbiased reference spectra for a match with the unbiased sample spectrum to identify the composition of the sample giving rise to said sample spectrum; and (ii) determining the concentration of the sample by relating the unbiased sample spectrum with the unbiased reference spectrum for a standard containing the component of interest, said standard being a known compound of a known concentration.
Claim 28
Dependent Claim
137 words
An improved method of determining a response factor for a quantitative model to determine composition of a sample by using a spectrum of a mixture of components wherein the components are of known concentration and a spectrum of each pure component that comprises the mixture, wherein the improvement comprises: a) normalizing the spectrum of the mixture and the spectra of the pure components to the largest spectral response in each respective spectrum; b) determining a predicted concentration of each component in the mixture from the spectrum of the mixture and the spectra of each pure component in the mixture using the quantitative model; and c) determining a response factor for each pure component in the mixture by ratioing the predicted concentration of each component in the mixture to the known concentration of each component in the mixture.
Claim 29
Dependent Claim
137 words
An improved method of determining a response factor for a quantitative model to determine composition of a sample by using a spectrum of a mixture of components wherein the components are of known concentration and a spectrum of each pure component that comprises the mixture, wherein the improvement comprises: a) normalizing the spectrum of the mixture and the spectra of the pure components to the largest spectral response in each respective spectrum; b) determining a predicted concentration of each component in the mixture from the spectrum of the mixture and the spectra of each pure component in the mixture using the quantitative model; and c) determining a response factor for each pure component in the mixture by ratioing the predicted concentration of each component in the mixture to the known concentration of each component in the mixture.
Claim 30
Dependent Claim
48 words
The method of claim 25 further comprising the step of normalizing each response factor for each pure component in the mixture to the response factor of one of the pure components in the mixture in order to determine a relative response factor for each component in the mixture.
Claim 31
Dependent Claim
32 words
The method of claim 25 further comprising the step of using the response factors to determine the concentration of components in an unknown mixture comprised of a combination of the pure components.
Claim 32
Dependent Claim
33 words
The method of claim 26 further comprising the step of using the relative response factors to determine the concentration of components in an unknown mixture comprised of a combination of the pure components.
Claim 33
Dependent Claim
22 words
The method of claim 1 wherein the transfer function is derived from the unbiased reference spectrum and a single biased working spectrum.
Claim 34
Dependent Claim
22 words
The method of claim 11 wherein the transfer function is derived from the unbiased reference spectrum and a single biased working spectrum.
Claim 35
Dependent Claim
22 words
The method of claim 23 wherein the transfer function is derived from the unbiased reference spectrum and a single biased working spectrum.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-08-13 | 2000-02-12 | 2000-08-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-08-13 | 2004-02-12 | 2004-08-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-08-13 | 2008-02-12 | 2008-08-13 | $7700.00 | Window Closed / Lapsed |