US5606045A
Expired / Lapsed
US
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Nucleic acid probes and methods
Assignee: Diatron Corp
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Filing: 1993-04-21
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Granted: 1997-02-25
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Expires: 2013-04-21
Abstract
Abstract The present invention is directed to compositions comprising an oligonucleotide linked to a detectably labeled marker component comprising a fluorophore moiety which comprises a substantially planar, multidentate macrocyclic ligand coordinated to a central atom capable of coordinating with two axial ligands and two polyoxyhydrocarbyl moieties which are attached as axial ligands to the central atom. The present invention is also directed to nucleic acid hybridization and amplification methods employing such compositions.
Inventors
- 👤 Walter B. Dandliker
- 👤 Robert F. Devlin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 00:19
CPC Classifications
A; A61; A61K; A61K49/00; A61K49/001; A61K49/0013; A61K49/0017; A61K49/0019; A61K49/0021; A61K41/00; A61K41/0057; A61K41/0071; A61K47/00; A61K47/50; A61K47/51; A61K47/54; A61K47/549; A61K47/56; A61K47/59; A61K47/60; A61K49/0015; A61K49/0036; A61K49/005; A61K49/0054; C; C07; C07D; C07D487/00; C07D487/22; C07J; C07J43/00; C07J43/003; C07J51/00; C09; C09B; C09B47/00; C09B47/04; C09B47/045; C09B47/06; C09B47/073; C09B47/08; C09B47/24; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N33/58; G01N33/582; G01N2333/00; G01N2333/005; G01N2333/08; G01N2333/18; G01N2333/19
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
75 words
A composition-comprising an oligonucleotide linked to a detectably labeled marker component which comprises a fluorophore moiety which is linked to two solubilizing polyoxyhydrocarbyl moieties, one located on either side of the fluorophore moiety, wherein said solubilizing polyoxyhydrocarbyl moieties are selected from a polyether, a polyol, or a water soluble polymer, and wherein said fluorophore moiety is a porphyrin derivative or an azaporphyrin derivative wherein one or more bridging carbon atoms has been replaced by nitrogen.
Claim 2
Dependent Claim
75 words
A composition-comprising an oligonucleotide linked to a detectably labeled marker component which comprises a fluorophore moiety which is linked to two solubilizing polyoxyhydrocarbyl moieties, one located on either side of the fluorophore moiety, wherein said solubilizing polyoxyhydrocarbyl moieties are selected from a polyether, a polyol, or a water soluble polymer, and wherein said fluorophore moiety is a porphyrin derivative or an azaporphyrin derivative wherein one or more bridging carbon atoms has been replaced by nitrogen.
Claim 3
Dependent Claim
22 words
The composition of claim 1 wherein said luminescent substantially planar molecular structure comprises a macrocyclic multidentate ligand coordinated to a central atom.
Claim 4
Dependent Claim
29 words
The composition of claim 1 wherein one of said two solubilizing polyoxyhydrocarbyl moieties is linked on either side of the plane of the macrocyclic ligand to the central atom.
Claim 5
Dependent Claim
20 words
The composition of claim 1 wherein said two solubilizing polyoxyhydrocarbyl moieties comprise axial ligands which coordinate to the central atom.
Claim 6
Dependent Claim
16 words
The composition of claim 4 wherein said central atom is capable of forming octahedral coordination complexes.
Claim 7
Dependent Claim
15 words
The composition of claim 2 wherein said macrocyclic multidentate ligand has a conjugated π-electron system.
Claim 8
Dependent Claim
14 words
The composition of claim 6 wherein said macrocyclic multidentate ligand comprises a nitrogen-containing macrocycle.
Claim 9
Dependent Claim
22 words
The composition of claim 7 wherein said central atom is selected from the group consisting of silicon, germanium, phosphorus, tin and zinc.
Claim 10
Dependent Claim
26 words
The composition of claim 7 wherein said macrocycle comprises a porphyrin derivative wherein from 1 to 4 of the bridging carbon atoms is replaced by nitrogen.
Claim 11
Dependent Claim
19 words
The composition of claim 4 further comprising a solubilizing polyoxyhydrocarbyl moiety comprised of polyethylene glycol or polyethylene glycol derivatives.
Claim 12
Dependent Claim
20 words
The composition of claim 10 wherein each said polyoxyhydrocarbyl moieties has a molecular weight of about 200 to about 20,000.
Claim 13
Dependent Claim
12 words
The composition of claim 9 wherein said macrocycle comprises a tetrabenzotriazaporphyrin derivative.
Claim 14
Dependent Claim
19 words
The composition of claim 12 wherein said macrocycle is selected from the group consisting of tetrabenzotriazaporphyrin, 27-phenyltetrabenzotriazaporphyrin, and 27-(p-methylphenyl)tetrabenzotriazaporphyrin.
Claim 15
Dependent Claim
11 words
The composition of claim 8 wherein the central atom is silicon.
Claim 16
Dependent Claim
28 words
The composition of claim 9 wherein said macrocyclic ligand has a low degree of symmetry so as to enhance the polarization of emission parallel to polarization of absorption.
Claim 17
Dependent Claim
13 words
The composition of claim 15 wherein said central atom is silicon or germanium.
Claim 18
Dependent Claim
17 words
The composition of claim 16 wherein said macrocyclic ligand has a lower symmetry than D 4h .
Claim 19
Dependent Claim
16 words
The composition of claim 17 wherein said macrocyclic ligand has at least one fused aromatic ring.
Claim 20
Dependent Claim
26 words
The composition of claim 17 wherein said macrocyclic ligand comprises a porphyrin derivative wherein 1 to 3 of the bridging carbon atoms is replaced by nitrogen.
Claim 21
Dependent Claim
13 words
The composition of claim 18 wherein said macrocyclic ligand comprises a phthalocyanine derivative.
Claim 22
Dependent Claim
12 words
The composition of claim 18 wherein said macrocycle comprises a tetrabenzotriazaporphyrin derivative.
Claim 23
Dependent Claim
13 words
The composition of claim 1 wherein said fluorophore moiety comprises a tetrabenzotriazaporphyrin derivative.
Claim 24
Dependent Claim
13 words
The composition of claim 1 wherein said fluorophore moiety comprises a phthalocyanine derivative.
Claim 25
Dependent Claim
27 words
The composition of claim 1 wherein said detectably labeled marker component has a decay time in the range of from about 1 nanosecond to about 50 nanoseconds.
Claim 26
Dependent Claim
22 words
The composition of claim 24 wherein said decay time is in the range of from about 5 nanoseconds to about 20 nanoseconds.
Claim 27
Dependent Claim
25 words
The composition of claim 1, 20 or 23 wherein said detectably labeled marker component comprises the caged dicarboxy silicon phthalocyanine dye shown in FIG. 1.
Claim 28
Dependent Claim
17 words
The composition of claim 1 wherein said oligonucleotide having a length of between 5 to 50 bases.
Claim 29
Dependent Claim
14 words
The composition of claim 1 wherein said linkage comprises (CH 2 ) 6 O.
Claim 30
Dependent Claim
14 words
The composition of claim 1 wherein said linkage comprises (CH 2 ) 2 NH.
Claim 31
Dependent Claim
10 words
The composition of claim 1 wherein said composition is thermostable.
Claim 32
Dependent Claim
10 words
The composition of claim 26 wherein said composition is thermostable.
Claim 33
Dependent Claim
20 words
The composition of claim 30 or 31 wherein said composition is stable after exposure to 90° C. for 1 hour.
Claim 34
Dependent Claim
20 words
The composition of claim 30 or 31 wherein said composition is stable after exposure to 100° C. for 10 minutes.
Claim 35
Dependent Claim
71 words
A composition comprising an oligonucleotide linked to a detectably labeled marker component which comprises a fluorophore moiety coupled to two solubilizing polyoxyhydrocarbyl moieties, one located on either side of the fluorophore moiety, wherein said fluorophore moiety comprises a macrocyclic multidentate ligand coordinated to a central atom; and wherein one of said two solubilizing polyoxyhydrocarbyl moieties is linked on either side of the plane of the macrocyclic ligand to the central atom.
Claim 36
Dependent Claim
71 words
A composition comprising an oligonucleotide linked to a detectably labeled marker component which comprises a fluorophore moiety coupled to two solubilizing polyoxyhydrocarbyl moieties, one located on either side of the fluorophore moiety, wherein said fluorophore moiety comprises a macrocyclic multidentate ligand coordinated to a central atom; and wherein one of said two solubilizing polyoxyhydrocarbyl moieties is linked on either side of the plane of the macrocyclic ligand to the central atom.
References Cited (Backward Citations)
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