Sensor with improved drift stability

Claim 2

A sensor for measuring the concentration of an analyte in a medium comprising: a sensing element comprising a sensing composition having an aqueous first phase comprising a pH sensitive indicator component in a buffer solution, wherein said buffer solution comprises less than 100 mM of a bicarbonate ion, wherein the concentration of said pH sensitive indicator component in said sensing composition is effective to provide a signal in response to the concentration of carbon dioxide in a medium to which said sensing composition is exposed, said carbon dioxide being effective to alter the pH of said aqueous phase, and a hydrophobic second phase which is permeable to said analyte and impermeable to ionized hydrogen, wherein said aqueous phase is dispersed within said hydrophobic phase, and wherein said sensing composition is substantially free of partitioning species, other than the analyte of interest, which migrate between said phases in response to a change in pH in said aqueous phase or between the sensing composition and the medium and which migration substantially affects said concentration dependent signal; an excitation assembly positioned and adapted to provide an excitation signal to said sensing element; a detector assembly positioned and adapted to detect an emitted signal from said sensing element, said sensing element being capable of providing said emitted signal in response to being exposed to said excitation signal; and a processor assembly positioned and adapted to analyze said emitted signal in determining the concentration of said analyte in said medium.

Claim 3

A sensor according to claim 1, wherein said sensing composition, immediately after being equilibrated in a first medium having a pCO 2 of 0.25 mmHg, provides a measurement of CO 2 partial pressure which drifts less than 6% over a three hour period when moved from said first medium to a second medium having a pCO 2 of 45.6 mmHg.

Claim 4

A sensor according to claim 1, wherein said pH sensitive indicator component is present in said aqueous phase between a concentration of 2 and 10 mM and is selected from the group consisting of 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein; 5-(and -6)-carboxy-2',7'-dichlorofluorescein; 5-(and -6)-carboxy-4',5'-dimethylfluorescein; carboxy5'(and 6')-carboxy-3,10-dihydroxy-spiro[7H-benzo[c]xanthene-7,1'(3'H)-isobenzofuran]-3'-one; carboxy5'(and 6')-carboxy-9-chloro-3,10-dihydroxy-spiro[7H-benzo[c]xanthene-7,1'(3'H)-isobenzofuran]-3'-one; carboxy5'(and 6')-carboxy-10-dimethylamino-3-hydroxy-spiro[7H-benzo[c]xanthene-7,1'(3'H)-isobenzofuran]-3'-one; carboxy5'(and 6')-carboxy-10-diethylamino-3-hydroxy-spiro[7H-benzo[c]xanthene-7,1'(3'H)-isobenzofuran]-3'-one; carboxy5'(and 6')-carboxy-3-hydroxy-tetrahydroquinolizino[1,9-hi]spiro[7H-benzo[c]xanthene-7,1'(3'H)-isobenzofuran]-3'-one; 4-chloromethyl-7-hydroxycoumarin; 4-[2-chloro-6-(ethylamino)-7-methyl-3-oxo-3H-xanthen-9-yl]-1,3-benzene-dicarboxylic acid; dextran, 2',7'-bis(2-carboxyethyl)-5(and 6)-carboxy-fluorescein, anionic; dextran, 4-[2-chloro-6-(ethylamino)-7-methyl-3-oxo-3H-xanthen-9-yl]-1,3-benzene-dicarboxylic acid, anionic; dextran, 7-hydroxycoumarin, neutral; dextran, b-methylumbelliferone, neutral; dextran, 9-chloro-3,10-dihydroxy-spiro[7H-benzo[c]xanthene-7,1'(3'H)-isobenzofuran]-3'-one, anionic; dextran, 10-dimethylamino-3-hydroxy-spiro[7H-benzo[c]xanthene-7,1'(3'H)-isobenzofuran]-3'-one, anionic; 8-hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt; and 3,10-dihydroxy-spiro[7H-benzo[c]xanthene-7,1'(3'H)-isobenzofuran]-3-one.

Claim 5

A sensor according to claim 1, wherein said aqueous phase further comprises a emulsification enhancement agent selected from the group consisting of poly(ethylene oxide), poly(ethylene glycol), poly(acrylamide), poly(dimethylacrylamide), poly(vinylmethyl acetomide), poly(styrene sulfonate), poly(vinylalcohol), poly(vinylpyrollidone), poly(hydroxyethylacrylate), poly(hydroxymethylacrylate), poly(acrylic acid), poly(vinyl methyl ether), dextran, hydroxyethylcellulose, hydroxypropyl cellulose, carboxymethylcellulose, methylcellulose, hydroxypropyl(methylcellulose), hydroxybutyl(methylcellulose), xanthan gum, guar gum, alginic acid, and carageenen, wherein said agent is present in an concentration between 1 and 50 weight percent based on the total aqueous phase components, and wherein said sensor comprises less than 3 mM of acidic or basic partitioning species, based on the volume of said aqueous phase, throughout the operating range of the sensor.

Claim 6

A sensor according to claim 1, wherein said sensor comprises less than 1 mM of an acidic partitioning species, based on the volume of said aqueous phase, throughout the operating range of the sensor.

Claim 7

A sensor according to claim 1, wherein said sensor comprises less than 1 mM of a basic partitioning species, based on the volume of said aqueous phase, throughout the operating range of the sensor.

Claim 8

A sensor for measuring the concentration of an analyte in a medium comprising: a sensing element comprising a sensing composition having an aqueous first phase comprising a pH sensitive indicator component, wherein the concentration of said pH sensitive indicator component in said sensing composition is effective to provide a signal in response to the concentration of carbon dioxide in a medium to which said sensing composition is exposed, said carbon dioxide being effective to alter the pH of said aqueous phase, and a hydrophobic second phase which is permeable to said analyte and impermeable to ionized hydrogen, wherein said aqueous phase is dispersed within said hydrophobic phase, and wherein said sensing composition comprises less than 1 mM of acidic or basic partitioning species, other than the analyte of interest, which migrate between said phases in response to a change in pH in said aqueous phase or between the sensing composition and the medium and which migration substantially affects said concentration dependent signal; an excitation assembly positioned and adapted to provide an excitation signal to said sensing element; a detector assembly positioned and adapted to detect an emitted signal from said sensing element, said sensing element being capable of providing said emitted signal in response to being exposed to said excitation signal; and a processor assembly positioned and adapted to analyze said emitted signal in determining the concentration of said analyte in said medium.

Claim 9

A sensor for measuring the concentration of an analyte in a medium comprising: a sensing element comprising a sensing composition having an aqueous first phase comprising a pH sensitive indicator component, wherein the concentration of said pH sensitive indicator component in said sensing composition is effective to provide a signal in response to the concentration of carbon dioxide in a medium to which said sensing composition is exposed, said carbon dioxide being effective to alter the pH of said aqueous phase, and a hydrophobic second phase which is permeable to said analyte and impermeable to ionized hydrogen, wherein said aqueous phase is dispersed within said hydrophobic phase, and wherein said sensing composition comprises less than 1 mM of acidic or basic partitioning species, other than the analyte of interest, which migrate between said phases in response to a change in pH in said aqueous phase or between the sensing composition and the medium and which migration substantially affects said concentration dependent signal; an excitation assembly positioned and adapted to provide an excitation signal to said sensing element; a detector assembly positioned and adapted to detect an emitted signal from said sensing element, said sensing element being capable of providing said emitted signal in response to being exposed to said excitation signal; and a processor assembly positioned and adapted to analyze said emitted signal in determining the concentration of said analyte in said medium.

Claim 10

A sensor according to claim 7, wherein said aqueous phase comprises a buffer solution equivalent in composition to a solution prepared with up to 100 mM of a bicarbonate salt, and wherein said sensing composition, immediately after being equilibrated in a first medium having a pCO 2 of 0.25 mmHg, provides a measurement of CO 2 partial pressure which drifts less than 3% over a three hour period when moved from said first medium to a second medium having a pCO 2 of 45.6 mmHg.

Claim 11

A sensor for measuring the concentration of an analyte in a medium comprising: a sensing element comprising a sensing composition having an aqueous first phase comprising between an effective amount and 20 mM of a pH sensitive indicator component in a buffer solution, wherein said buffer solution has a buffering capacity equivalent to a solution prepared with between 20 and 100 mM of a bicarbonate salt and up to 50 mM of a 50:50 mixture of a monobasic and dibasic phosphate salt, wherein said pH sensitive indicator component is effective to provide a signal in response to the concentration of carbon dioxide in a medium to which said sensing composition is exposed, said carbon dioxide being effective to alter the pH of said aqueous phase, and a hydrophobic second phase which is permeable to said analyte and impermeable to ionized hydrogen, wherein said aqueous phase is dispersed within said hydrophobic phase, wherein said sensing composition is substantially free of acidic partitioning species, other than the analyte of interest, which migrate between said phases in response to a change in pH in said aqueous phase or between the sensing composition and the medium and which migration substantially affects said concentration dependent signal, wherein said sensing composition, immediately after being equilibrated in a first medium having a pCO 2 of 0.25 mmHg, provides a measurement of CO 2 partial pressure which drifts less than 12% over a three hour period when moved from said first medium to a second medium having a pCO 2 of 45.6 mmHg, and wherein said sensing composition has a specific sensitivity bwtween 0.2 and 0.5; an excitation assembly positioned and adapted to provide an excitation signal to said sensing element; a detector assembly positioned and adapted to detect an emitted signal from said sensing element, said sensing element being capable of providing said emitted signal in response to being exposed to said excitation signal; and a processor assembly positioned and adapted to analyze said emitted signal in determining the concentration of said analyte in said medium.

Claim 12

A sensor for measuring the concentration of an analyte in a medium comprising: a sensing element comprising a sensing composition having an aqueous first phase comprising between an effective amount and 20 mM of a pH sensitive indicator component in a buffer solution, wherein said buffer solution has a buffering capacity equivalent to a solution prepared with between 20 and 100 mM of a bicarbonate salt and up to 50 mM of a 50:50 mixture of a monobasic and dibasic phosphate salt, wherein said pH sensitive indicator component is effective to provide a signal in response to the concentration of carbon dioxide in a medium to which said sensing composition is exposed, said carbon dioxide being effective to alter the pH of said aqueous phase, and a hydrophobic second phase which is permeable to said analyte and impermeable to ionized hydrogen, wherein said aqueous phase is dispersed within said hydrophobic phase, wherein said sensing composition is substantially free of acidic partitioning species, other than the analyte of interest, which migrate between said phases in response to a change in pH in said aqueous phase or between the sensing composition and the medium and which migration substantially affects said concentration dependent signal, wherein said sensing composition, immediately after being equilibrated in a first medium having a pCO 2 of 0.25 mmHg, provides a measurement of CO 2 partial pressure which drifts less than 12% over a three hour period when moved from said first medium to a second medium having a pCO 2 of 45.6 mmHg, and wherein said sensing composition has a specific sensitivity bwtween 0.2 and 0.5; an excitation assembly positioned and adapted to provide an excitation signal to said sensing element; a detector assembly positioned and adapted to detect an emitted signal from said sensing element, said sensing element being capable of providing said emitted signal in response to being exposed to said excitation signal; and a processor assembly positioned and adapted to analyze said emitted signal in determining the concentration of said analyte in said medium.

Claim 13

A sensor according to claim 9, wherein said buffer solution is equivalent in composition to a solution prepared with between 40 and 80 mM of a bicarbonate salt.

Claim 14

A sensor according to claim 9, wherein said sensor comprises less than 1 mM of an acidic partitioning species and less than 3 mM of a basic partitioning species, based on the volume of said aqueous phase, throughout the operating range of the sensor, and wherein said analyte is carbon dioxide, said indicator component is a salt hydroxypyrene trisulfonic acid, and said hydrophobic phase comprises silicone.

Claim 15

A sensor for measuring the concentration of an analyte in a medium comprising: a sensing element comprising a sensing composition having an aqueous first phase comprising between an effective amount and 20 mM of a pH sensitive indicator component in a buffer solution, wherein said buffer solution has a buffering capacity equivalent to a solution prepared with at least 20 mM of a bicarbonate salt and up to 50 mM of a 50:50 mixture of a monobasic and dibasic phosphate salt, wherein said pH sensitive indicator component is effective to provide a signal in response to the concentration of carbon dioxide in a medium to which said sensing composition is exposed, said carbon dioxide being effective to alter the pH of said aqueous phase, and a hydrophobic second phase which is permeable to said analyte and impermeable to ionized hydrogen, wherein said aqueous phase is dispersed within said hydrophobic phase, wherein said sensing composition is substantially free of acidic partitioning species, other than the analyte of interest, which migrate between said phases in response to a change in pH in said aqueous phase or between the sensing composition and the medium and which migration substantially affects said concentration dependent signal, wherein said sensing composition, immediately after being equilibrated in a first medium having a pCO 2 of 0.25 mmHg, provides a measurement of CO 2 partial pressure which drifts less than 12% over a three hour period when moved from said first medium to a second medium having a pCO 2 of 45.6 mmHg, wherein said sensing composition has a specific sensitivity between 0.2 and 0.5, and wherein said aqueous phase does not include the combination of hydroxypyrene trisulfonic acid and polyvinylpyrolidone; an excitation assembly positioned and adapted to provide an excitation signal to said sensing element; a detector assembly positioned and adapted to detect an emitted signal from said sensing element, said sensing element being capable of providing said emitted signal in response to being exposed to said excitation signal; and a processor assembly positioned and adapted to analyze said emitted signal in determining the concentration of said analyte in said medium.

Claim 16

A sensor for measuring the concentration of an analyte in a medium comprising: a sensing element comprising a sensing composition having an aqueous first phase comprising between an effective amount and 20 mM of a pH sensitive indicator component in a buffer solution, wherein said buffer solution has a buffering capacity equivalent to a solution prepared with at least 20 mM of a bicarbonate salt and up to 50 mM of a 50:50 mixture of a monobasic and dibasic phosphate salt, wherein said pH sensitive indicator component is effective to provide a signal in response to the concentration of carbon dioxide in a medium to which said sensing composition is exposed, said carbon dioxide being effective to alter the pH of said aqueous phase, and a hydrophobic second phase which is permeable to said analyte and impermeable to ionized hydrogen, wherein said aqueous phase is dispersed within said hydrophobic phase, wherein said sensing composition is substantially free of acidic partitioning species, other than the analyte of interest, which migrate between said phases in response to a change in pH in said aqueous phase or between the sensing composition and the medium and which migration substantially affects said concentration dependent signal, wherein said sensing composition, immediately after being equilibrated in a first medium having a pCO 2 of 0.25 mmHg, provides a measurement of CO 2 partial pressure which drifts less than 12% over a three hour period when moved from said first medium to a second medium having a pCO 2 of 45.6 mmHg, wherein said sensing composition has a specific sensitivity between 0.2 and 0.5, and wherein said aqueous phase does not include the combination of hydroxypyrene trisulfonic acid and polyvinylpyrolidone; an excitation assembly positioned and adapted to provide an excitation signal to said sensing element; a detector assembly positioned and adapted to detect an emitted signal from said sensing element, said sensing element being capable of providing said emitted signal in response to being exposed to said excitation signal; and a processor assembly positioned and adapted to analyze said emitted signal in determining the concentration of said analyte in said medium.

Claim 17

A sensor according to claim 12, wherein said sensing composition, immediately after being equilibrated in a first medium having a pCO 2 of 0.25 mmHg, provides a measurement of CO 2 partial pressure which drifts less than 3% over a three hour period when moved from said first medium to a second medium having a pCO 2 of 45.6 mmHg, and wherein said buffer solution is equivalent in composition to a solution prepared with at least 50 mM of a bicarbonate salt.

Claim 18

A sensor according to claim 12, wherein said sensor comprises less than 1 mM of an acidic partitioning species and less than 3 mM of a basic partitioning species, based on the volume of said aqueous phase, throughout the operating range of the sensor, and wherein said analyte is carbon dioxide, said indicator component is a salt of hydroxypyrene trisulfonic acid, and said hydrophobic phase comprises silicone.

Claim 19

A sensor for measuring the concentration of an analyte in a medium comprising: a sensing element comprising a sensing composition having an aqueous first phase comprising a pH sensitive indicator component in a buffer solution, wherein said buffer solution has a buffering capacity equivalent to a solution prepared with at least one of a bicarbonate salt and a 50:50 mixture of a monobasic phosphate salt and a dibasic phosphate salt, wherein the sum of the molar concentration of the indicator component and one-half of the molar concentration of the mixture of phosphate salts divided by the molar concentration of the bicarbonate salt is at least 0.66, wherein said pH sensitive indicator component is effective to provide a signal in response to the concentration of carbon dioxide in a medium to which said sensing composition is exposed, said carbon dioxide being effective to alter the pH of said aqueous phase, and a hydrophobic second phase which is permeable to said analyte and impermeable to ionized hydrogen, wherein said aqueous phase is dispersed within said hydrophobic phase, wherein said sensing composition is substantially free of basic partitioning species, other than the analyte of interest, which migrate between said phases in response to a change in pH in said aqueous phase or between the sensing composition and the medium and which migration substantially affects said concentration dependent signal, wherein said sensing composition, immediately after being equilibrated in a first medium having a pCO 2 of 0.25 mmHg, provides a measurement of CO 2 partial pressure which drifts less than 12% over a three hour period when moved from said first medium to a second medium having a pCO 2 of 45.6 mmHg, and wherein said sensing composition has a specific sensitivity between 0.2 and 0.5; an excitation assembly positioned and adapted to provide an excitation signal to said sensing element; a detector assembly positioned and adapted to detect an emitted signal from said sensing element, said sensing element being capable of providing said emitted signal in response to being exposed to said excitation signal; and a processor assembly positioned and adapted to analyze said emitted signal in determining the concentration of said analyte in said medium.

Claim 20

A sensor for measuring the concentration of an analyte in a medium comprising: a sensing element comprising a sensing composition having an aqueous first phase comprising a pH sensitive indicator component in a buffer solution, wherein said buffer solution has a buffering capacity equivalent to a solution prepared with at least one of a bicarbonate salt and a 50:50 mixture of a monobasic phosphate salt and a dibasic phosphate salt, wherein the sum of the molar concentration of the indicator component and one-half of the molar concentration of the mixture of phosphate salts divided by the molar concentration of the bicarbonate salt is at least 0.66, wherein said pH sensitive indicator component is effective to provide a signal in response to the concentration of carbon dioxide in a medium to which said sensing composition is exposed, said carbon dioxide being effective to alter the pH of said aqueous phase, and a hydrophobic second phase which is permeable to said analyte and impermeable to ionized hydrogen, wherein said aqueous phase is dispersed within said hydrophobic phase, wherein said sensing composition is substantially free of basic partitioning species, other than the analyte of interest, which migrate between said phases in response to a change in pH in said aqueous phase or between the sensing composition and the medium and which migration substantially affects said concentration dependent signal, wherein said sensing composition, immediately after being equilibrated in a first medium having a pCO 2 of 0.25 mmHg, provides a measurement of CO 2 partial pressure which drifts less than 12% over a three hour period when moved from said first medium to a second medium having a pCO 2 of 45.6 mmHg, and wherein said sensing composition has a specific sensitivity between 0.2 and 0.5; an excitation assembly positioned and adapted to provide an excitation signal to said sensing element; a detector assembly positioned and adapted to detect an emitted signal from said sensing element, said sensing element being capable of providing said emitted signal in response to being exposed to said excitation signal; and a processor assembly positioned and adapted to analyze said emitted signal in determining the concentration of said analyte in said medium.

Claim 21

A sensor according to claim 15, wherein said sensor comprises less than 3 mM of an acidic partitioning species and less than 1 mM of a basic partitioning species, based on the volume of said aqueous phase, throughout the operating range of the sensor, and wherein said sensing composition, immediately after being equilibrated in a first medium having a pCO 2 of 0.25 mmHg, provides a measurement of CO 2 partial pressure which drifts less than 6% over a three hour period when moved from said first medium to a second medium having a pCO 2 of 45.6 mmHg.

Claim 22

A sensor according to claim 15, wherein said analyte is carbon dioxide, said indicator component is a salt of hydroxypyrene trisulfonic acid, and said hydrophobic phase comprises silicone, and wherein said sensor comprises less than 1 mM of an acidic partitioning species and less than 0.5 mM of a basic partitioning species, based on the volume of said aqueous phase, throughout the operating range of the sensor.

Claim 23

A sensor according to claims 1, 9, or 15, wherein said sensor is of such a size as to be capable of being intravenously introduced into the body of a patient.

Claim 24

A sensor according to claims 7, 9, or 15, wherein said sensor further comprises a cassette comprising a flow through housing.

Claim 25

A sensor for measuring the concentration of an analyte in a medium comprising: a sensing element comprising a sensing composition having an aqueous first phase comprising a pH sensitive indicator component in a buffer solution, wherein said buffer solution has a buffering capacity equivalent to a solution prepared with at least 15 mM of a bicarbonate salt and up to 50 mM of a 50:50 mixture of a monobasic and dibasic phosphate salt, wherein the ratio of the sum of the molar concentration of the indicator component and one-half of the molar concentration of the mixture of phosphate salts divided by the molar concentration of the bicarbonate salt is at least 0.66, wherein said pH sensitive indicator component is effective to provide a signal in response to the concentration of carbon dioxide in a medium to which said sensing composition is exposed, said carbon dioxide being effective to alter the pH of said aqueous phase, and a hydrophobic second phase which is permeable to said analyte and impermeable to ionized hydrogen, wherein said aqueous phase is dispersed within said hydrophobic phase, wherein said sensing composition, immediately after being equilibrated in a first medium having a pCO 2 of 0.25 mmHg, provides a measurement of CO 2 partial pressure which drifts less than 12% over a three hour period when moved from said first medium to a second medium having a pCO 2 of 45.6 mmHg, and wherein said sensing composition has a specific sensitivity between 0.2 and 0.5; an excitation assembly positioned and adapted to provide an excitation signal to said sensing element; a detector assembly positioned and adapted to detect an emitted signal from said sensing element, said sensing element being capable of providing said emitted signal in response to being exposed to said excitation signal; and a processor assembly positioned and adapted to analyze said emitted signal in determining the concentration of said analyte in said medium.

Claim 26

A sensor for measuring the concentration of an analyte in a medium comprising: a sensing element comprising a sensing composition having an aqueous first phase comprising a pH sensitive indicator component in a buffer solution, wherein said buffer solution has a buffering capacity equivalent to a solution prepared with at least 15 mM of a bicarbonate salt and up to 50 mM of a 50:50 mixture of a monobasic and dibasic phosphate salt, wherein the ratio of the sum of the molar concentration of the indicator component and one-half of the molar concentration of the mixture of phosphate salts divided by the molar concentration of the bicarbonate salt is at least 0.66, wherein said pH sensitive indicator component is effective to provide a signal in response to the concentration of carbon dioxide in a medium to which said sensing composition is exposed, said carbon dioxide being effective to alter the pH of said aqueous phase, and a hydrophobic second phase which is permeable to said analyte and impermeable to ionized hydrogen, wherein said aqueous phase is dispersed within said hydrophobic phase, wherein said sensing composition, immediately after being equilibrated in a first medium having a pCO 2 of 0.25 mmHg, provides a measurement of CO 2 partial pressure which drifts less than 12% over a three hour period when moved from said first medium to a second medium having a pCO 2 of 45.6 mmHg, and wherein said sensing composition has a specific sensitivity between 0.2 and 0.5; an excitation assembly positioned and adapted to provide an excitation signal to said sensing element; a detector assembly positioned and adapted to detect an emitted signal from said sensing element, said sensing element being capable of providing said emitted signal in response to being exposed to said excitation signal; and a processor assembly positioned and adapted to analyze said emitted signal in determining the concentration of said analyte in said medium.

Claim 27

A sensor according to claim 20, wherein said buffer solution ratio is at least 1, and wherein said sensing composition, immediately after being equilibrated in a first medium having a pCO 2 of 0.25 mmHg, provides a measurement of CO 2 partial pressure which drifts less than 6% over a three hour period when moved from said first medium to a second medium having a pCO 2 of 45.6 mmHg.

Claim 28

A sensor according to claims 1, 12, or 20, wherein said sensor has a response time less than 1 minute.

Claim 29

A sensor according to claims 7, 12, or 20, wherein said sensor is maintained in an air-equilibrated buffer prior to temporal physiological sampling.

Claim 30

A sensor according to claim 12, 15, or 20, wherein said sensor further comprises an optical fiber.