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US5698397A Expired / Lapsed US ⏱️ 0.0 years remaining

Up-converting reporters for biological and other assays using laser excitation techniques

Assignee: SRI International Inc
•
Filing: 1995-06-07
•
Granted: 1997-12-16
•
Expires: 2015-06-07
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Abstract

Abstract The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits radiation at one or more higher frequencies, providing enhanced signal-to-noise ratio and the essential elimination of background sample autofluorescence. The methods, compositions, and apparatus are suitable for the sensitive detection of multiple analytes and for various clinical and environmental sampling techniques.

Inventors

  • 👤 David A. Zarling
  • 👤 Michel J. Rossi
  • 👤 Norman A. Peppers
  • 👤 James Kane
  • 👤 Gregory W. Faris
  • 👤 Mark J. Dyer
  • 👤 Steve Y. Ng
  • 👤 Luke V. Schneider

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-25 16:01
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/645; B; B82; B82Y; B82Y15/00; C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6818; G01N21/6428; G01N21/6452; G01N21/6454; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N33/536; G01N33/542; G01N33/58; G01N33/582; B01; B01J; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00279; B01J2219/00306; B01J2219/00313; B01J2219/00315; B01J2219/00583; B01J2219/00603; B01J2219/00605; B01J2219/00646; B01J2219/00648; B01J2219/00659; B01J2219/0068; B01J2219/00702; B01J2219/00707; C40; C40B; C40B60/00; C40B60/14; G01N2021/6417; G01N2021/6419

Claims Analysis

Total Claims: 14
Claim 1
Independent Claim 50 words
A composition comprising an up-converting inorganic phosphor which converts excitation radiation to emission radiation of a shorter wavelength and comprises at least one rare earth element in a host material and a probe selected from the group consisting of: antibodies, avidins, lectins, Staphylococcus aureus Protein A, antigens, polypeptides and polynucleotides.
Claim 2
Dependent Claim 50 words
A composition comprising an up-converting inorganic phosphor which converts excitation radiation to emission radiation of a shorter wavelength and comprises at least one rare earth element in a host material and a probe selected from the group consisting of: antibodies, avidins, lectins, Staphylococcus aureus Protein A, antigens, polypeptides and polynucleotides.
Claim 3
Dependent Claim 23 words
A composition according to claim 1, wherein the up-converting inorganic phosphor comprises ytterbium and an emitter selected from erbium, holmium, thulium, and terbium.
Claim 4
Dependent Claim 19 words
A composition according to claim 1, wherein the probe is attached to an up-converting inorganic phosphor by noncovalent linkage.
Claim 5
Dependent Claim 24 words
A composition according to claim 1, wherein the probe is streptavidin and the up-converting inorganic phosphor is attached to the probe by noncovalent linkage.
Claim 6
Dependent Claim 24 words
A composition of claim 4 further comprising a biotinylated magnetic bead, a streptavidin-coated magnetic bead, an avidin-coated magnetic bead, or an immunoglobulin-coated magnetic bead.
Claim 7
Dependent Claim 27 words
A composition for diagnostic detection of an analyte, comprising a microcrystalline up-convening phosphor covalently bound to antibodies, avidins, lectins, Staphylococcus aureus Protein A. antigens, polypeptides and polynucleotides.
Claim 8
Dependent Claim 27 words
A composition for diagnostic detection of an analyte, comprising a microcrystalline up-convening phosphor covalently bound to antibodies, avidins, lectins, Staphylococcus aureus Protein A. antigens, polypeptides and polynucleotides.
Claim 9
Dependent Claim 97 words
A composition of claim 6, wherein the microcrystalline up-converting phosphor has the formula: Na(Y.sub.x Yb.sub.y Er.sub.z)F.sub.4 : wherein x is 0.7 to 0.9, y is 0.09 to 0.29, and z is 0.05 to 0.01; Na(Y.sub.x Yb.sub.y Ho.sub.z)F.sub.4 : wherein x is 0.7 to 0.9, y is 0.0995 to 0.2995, and z is 0.0005 to 0.001; Na(Y.sub.x Yb.sub.y Tm.sub.z)F.sub.4 : wherein x is 0.7 to 0.9, y is 0.0995 to 0.2995, and z is 0.0005 to 0.001; or (Y.sub.x Yb.sub.y Er.sub.z)O.sub.2 S: wherein x is 0.7 to 0.9, y is 0.05 to 0.12; z is 0.05 to 0.12.
Claim 10
Dependent Claim 163 words
A composition of claim 6, wherein the microcrystalline up-converting phosphor has a formula selected from the group consisting of: (Y 0 .80 Yb 0 .18 Er 0 .02 F 3 ; (Y 0 .87 Yb 0 .13 Tm 0 .001)F 3 ; (Y 0 .80 Yb 0 .198 Ho 0 .002)F 3 ; (Gd 0 .08 Yb 0 .18 Er 0 .02)F 3 ; (Gd 0 .87 Yb 0 .13 Tm 0 .001)F 3 ; (Gd 0 .80 Yb 0 .198 Ho 0 .002)F 3 ; (Y 0 .86 Yb 0 .08 Er 0 .06) 2 O 2 S; (Y 0 .87 Yb 0 .13 Tm 0 .001) 2 O 2 S; (Y 0 .08 Yb 0 .198 Ho 0 .0022)O 2 S; (Gd 0 .86 Yb 0 .08 Er 0 .06) 2 O 2 S; (Gd 0 .87 Yb 0 .13 Tm 0 .001) 2 O 2 S; and (Gd 0 .08 Yb 0 .198 Ho 0 .002) 2 O 2 S.
Claim 11
Dependent Claim 26 words
A composition for diagnostic detection of an analyte, comprising an up-converting phosphor non-covalently bound to antibodies, avidins, lectins, Staphylococcus aureus Protein A, antigens, polypeptides and polynucleotides.
Claim 12
Dependent Claim 26 words
A composition for diagnostic detection of an analyte, comprising an up-converting phosphor non-covalently bound to antibodies, avidins, lectins, Staphylococcus aureus Protein A, antigens, polypeptides and polynucleotides.
Claim 13
Dependent Claim 97 words
A composition of claim 9 wherein the microcrystalline up-converting phosphor has the formula: Na(Y.sub.x Yb.sub.y Er.sub.z)F.sub.4 : wherein x is 0.7 to 0.9, y is 0.09 to 0.29, and z is 0.05 to 0.01; Na(Y.sub.x Yb.sub.y Ho.sub.z)F.sub.4 : wherein x is 0.7 to 0.9, y is 0.0995 to 0.2995, and z is 0.0005 to 0.001; Na(Y.sub.x Yb.sub.y Tm.sub.z)F.sub.4 : wherein x is 0.7 to 0.9, y is 0.0995 to 0.2995, and z is 0.0005 to 0.001; or (Y.sub.x Yb.sub.y Er.sub.z)O.sub.2 S: wherein x is 0.7 to 0.9, y is 0.05 to 0.12; z is 0.05 to 0.12.
Claim 14
Dependent Claim 164 words
A composition of claim 9, wherein the microcrystalline up-converting phosphor has a formula selected from the group consisting of: (Y 0 .80 Yb 0 .18 Er 0 .02)F 3 ; (Y 0 .87 Yb 0 .13 Tm 0 .001)F 3 ; (Y 0 .80 Yb 0 .198 Ho 0 .002)F 3 ; (Gd 0 .80 Yb 0 .18 Er 0 .02)F 3 ; (Gd 0 .87 Yb 0 .13 Tm 0 .001)F 3 ; (Gd 0 .80 Yb 0 .198 Ho 0 .002)F 3 ; (Y 0 .86 Yb 0 .08 Er 0 .06) 2 O 2 S; (Y 0 .87 Yb 0 .13 Tm 0 .001) 2 O 2 S; (Y 0 .80 Yb 0 .198 Ho 0 .002) 2 O 2 S; (Gd 0 .86 Yb 0 .08 Er 0 .06) 2 O 2 S; (Gd 0 .87 Yb 0 .13 Tm 0 .001) 2 O 2 S; and (Gd 0 .08 Yb 0 .198 Ho 0 .002) 2 O 2 S.

References Cited (Backward Citations)

US3599109A Cited
US3634614A Cited
US3593055A Cited
US4206132A Cited
US4032351A Cited
US4100416A Cited
US4492751A Cited
US4228237A Cited
US4837169A Cited
GB2103362A Cited
EP0071859A2 Cited
US4695393A Cited
US4724217A Cited
US4666862A Cited
EP0174744A2 Cited
US4727020A Cited
US5043265A Cited
US4913883A Cited
US5066580A Cited
EP0476556A2 Cited
US5188942A Cited
US5141740A Cited
US5166948A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 2000-12-15 2001-06-16 2001-12-16 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 2004-12-15 2005-06-16 2005-12-16 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2008-12-15 2009-06-16 2009-12-16 $7700.00 Window Closed / Lapsed

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