Patent US5720862A successfully refreshed from Google Patents.
US5720862A
Expired / Lapsed
US
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Sensor and production method of and measurement method using the same
Assignee: Arkray Inc
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Filing: 1996-04-05
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Granted: 1998-02-24
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Expires: 2016-04-05
Abstract
Abstract There is provided a sensor for the measurement of a content of a material in liquid which material is oxidized with an oxidase enzyme in which sensor a reagent layer is formed on an electrode system composed of a measuring electrode and a counter electrode both of which are formed on an insulating substrate, the reagent layer is composed of a hydrophilic polymer layer comprising a hydrophilic polymer and a reactive layer comprising the oxidase enzyme and an electron carrier, and the reagent layer further comprises a phosphate.
Inventors
- 👤 Katsumi Hamamoto
- 👤 Hisashi Okuda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 13:57
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; C12Q1/005; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
86 words
A sensor for the measurement of a content of a material in liquid which material is oxidized with an oxidase enzyme in which sensor a reagent layer is formed on an electrode system composed of a measuring electrode and a counter electrode both of which are formed on an insulating substrate, and the reagent layer is composed of a hydrophilic polymer layer comprising a hydrophilic polymer and a reactive layer comprising the oxidase enzyme and an electron carrier wherein the reactive layer further comprises a phosphate.
Claim 2
Dependent Claim
86 words
A sensor for the measurement of a content of a material in liquid which material is oxidized with an oxidase enzyme in which sensor a reagent layer is formed on an electrode system composed of a measuring electrode and a counter electrode both of which are formed on an insulating substrate, and the reagent layer is composed of a hydrophilic polymer layer comprising a hydrophilic polymer and a reactive layer comprising the oxidase enzyme and an electron carrier wherein the reactive layer further comprises a phosphate.
Claim 3
Dependent Claim
30 words
The sensor according to claim 1 wherein the oxidase enzyme is lactic acid oxidase and the sensor is used for the measurement of a lactic acid concentration in the liquid.
Claim 4
Dependent Claim
18 words
The sensor according to claim 1 wherein the hydrophilic polymer is purified to at least 99.9% by weight.
Claim 5
Dependent Claim
105 words
A sensor for the measurement of a content of a material in liquid which material is oxidized with an oxidase enzyme in which sensor a reagent layer is formed on an electrode system composed of a measuring electrode and a counter electrode both of which are formed on an insulating substrate, and the reagent layer is composed of a hydrophilic polymer layer comprising a hydrophilic polymer and a reactive layer comprising the oxidase enzyme and an electron carrier wherein the reactive layer further comprises a phosphate, wherein at least one of the hydrophilic polymer layer and the reactive layer further comprises an alkylene oxide polymer.
Claim 6
Dependent Claim
105 words
A sensor for the measurement of a content of a material in liquid which material is oxidized with an oxidase enzyme in which sensor a reagent layer is formed on an electrode system composed of a measuring electrode and a counter electrode both of which are formed on an insulating substrate, and the reagent layer is composed of a hydrophilic polymer layer comprising a hydrophilic polymer and a reactive layer comprising the oxidase enzyme and an electron carrier wherein the reactive layer further comprises a phosphate, wherein at least one of the hydrophilic polymer layer and the reactive layer further comprises an alkylene oxide polymer.
Claim 7
Independent Claim
106 words
A method for the production of a sensor comprising the steps of: forming an electrode system composed of a measuring electrode and a counter electrode on an insulating substrate; coating at least a portion of the electrode system with a hydrophilic polymer layer by applying a solution containing a hydrophilic polymer to the electrode system followed by drying the solution; and coating at least a portion of the hydrophilic polymer layer with a reactive layer by applying a solution containing a composition for the reactive layer comprising an oxidase enzyme, an electron carrier and a phosphate to the hydrophilic polymer layer followed by drying the solution.
Claim 8
Independent Claim
106 words
A method for the production of a sensor comprising the steps of: forming an electrode system composed of a measuring electrode and a counter electrode on an insulating substrate; coating at least a portion of the electrode system with a hydrophilic polymer layer by applying a solution containing a hydrophilic polymer to the electrode system followed by drying the solution; and coating at least a portion of the hydrophilic polymer layer with a reactive layer by applying a solution containing a composition for the reactive layer comprising an oxidase enzyme, an electron carrier and a phosphate to the hydrophilic polymer layer followed by drying the solution.
Claim 9
Dependent Claim
36 words
The method for the production of the sensor according to claim 5 wherein the oxidase enzyme is lactic acid oxidase and the sensor is used for the measurement of a lactic acid concentration in the liquid.
Claim 10
Dependent Claim
24 words
The method for the production of the sensor according to claim 5 wherein the hydrophilic polymer is purified to at least 99.9% by weight.
Claim 11
Dependent Claim
31 words
The method for the production of the sensor according to claim 5 wherein the solution containing a composition for the reactive layer includes 0.01 to 1.00% by weight of the phosphate.
Claim 12
Independent Claim
132 words
A method for the production of a sensor comprising the steps of: forming an electrode system composed of a measuring electrode and a counter electrode on an insulating substrate; coating at least a portion of the electrode system with a hydrophilic polymer layer by applying a solution containing a hydrophilic polymer to the electrode system followed by drying the solution; and coating at least a portion of the hydrophilic polymer layer with a reactive layer by applying a solution containing a composition for the reactive layer comprising an oxidase enzyme, an electron carrier and a phosphate to the hydrophilic polymer layer followed by drying the solution, wherein at least one of the solution containing the hydrophilic polymer and the solution containing a composition for the reactive layer includes an alkylene oxide polymer.
Claim 13
Independent Claim
132 words
A method for the production of a sensor comprising the steps of: forming an electrode system composed of a measuring electrode and a counter electrode on an insulating substrate; coating at least a portion of the electrode system with a hydrophilic polymer layer by applying a solution containing a hydrophilic polymer to the electrode system followed by drying the solution; and coating at least a portion of the hydrophilic polymer layer with a reactive layer by applying a solution containing a composition for the reactive layer comprising an oxidase enzyme, an electron carrier and a phosphate to the hydrophilic polymer layer followed by drying the solution, wherein at least one of the solution containing the hydrophilic polymer and the solution containing a composition for the reactive layer includes an alkylene oxide polymer.
Claim 14
Dependent Claim
33 words
The method for the production of the sensor according to claim 9 wherein a concentration of the alkylene oxide polymer in the solution is in the range of 0.01 to 1.00% by weight.
Claim 15
Independent Claim
101 words
A method for measuring the concentration of lactic acid in a sample comprising: contacting a sample with a sensor in which a reagent layer is formed on an electrode system composed of a measuring electrode and a counter electrode both of which are formed on an insulating substrate, the reagent layer is composed of a hydrophilic polymer layer comprising a hydrophilic polymer and a reactive layer comprising lactic acid oxidase and an electron carrier, and the reactive layer further comprises a phosphate; obtaining a current value; and converting the current value into a concentration of the lactic acid in the sample.
Claim 16
Independent Claim
101 words
A method for measuring the concentration of lactic acid in a sample comprising: contacting a sample with a sensor in which a reagent layer is formed on an electrode system composed of a measuring electrode and a counter electrode both of which are formed on an insulating substrate, the reagent layer is composed of a hydrophilic polymer layer comprising a hydrophilic polymer and a reactive layer comprising lactic acid oxidase and an electron carrier, and the reactive layer further comprises a phosphate; obtaining a current value; and converting the current value into a concentration of the lactic acid in the sample.
Claim 17
Dependent Claim
90 words
A sensor for the measurement of a content of a material in liquid which material is oxidized with an oxidase enzyme in which sensor a reagent layer is formed on an electrode system composed of a measuring electrode and a counter electrode both of which are formed on an insulating substrate, and the reagent layer is composed of a hydrophilic polymer layer comprising a hydrophilic polymer and a reactive layer comprising the oxidase enzyme and an electron carrier wherein the hydrophilic polymer is purified to at least 99.9% by weight.
Claim 18
Dependent Claim
90 words
A sensor for the measurement of a content of a material in liquid which material is oxidized with an oxidase enzyme in which sensor a reagent layer is formed on an electrode system composed of a measuring electrode and a counter electrode both of which are formed on an insulating substrate, and the reagent layer is composed of a hydrophilic polymer layer comprising a hydrophilic polymer and a reactive layer comprising the oxidase enzyme and an electron carrier wherein the hydrophilic polymer is purified to at least 99.9% by weight.
Claim 19
Dependent Claim
97 words
A sensor for the measurement of a content of a material in liquid which material is oxidized with an oxidase enzyme in which sensor a reagent layer is formed on an electrode system composed of a measuring electrode and a counter electrode both of which are formed on an insulating substrate, and the reagent layer is composed of a hydrophilic polymer layer comprising a hydrophilic polymer and a reactive layer comprising the oxidase enzyme and an electron carrier wherein at least one of the hydrophilic polymer layer and the reactive layer further comprises an alkylene oxide polymer.
Claim 20
Dependent Claim
97 words
A sensor for the measurement of a content of a material in liquid which material is oxidized with an oxidase enzyme in which sensor a reagent layer is formed on an electrode system composed of a measuring electrode and a counter electrode both of which are formed on an insulating substrate, and the reagent layer is composed of a hydrophilic polymer layer comprising a hydrophilic polymer and a reactive layer comprising the oxidase enzyme and an electron carrier wherein at least one of the hydrophilic polymer layer and the reactive layer further comprises an alkylene oxide polymer.
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