Patent US5726565A successfully refreshed from Google Patents.
US5726565A
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Coulometric analysis method and a device therefor
Assignee: Nakano Vinegar Co Ltd
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Filing: 1995-08-04
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Granted: 1998-03-10
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Expires: 2015-08-04
Abstract
Abstract The present invention provides a rapid coulometric analysis for the quantitative determination of a sample substance wherein the coulometric analysis has high reproducibility without any application of voltage, etc., from an outside source, as well as a galvanic cell and a device for use in said coulometric analysis. According to the present invention, a wide variety of components can be analyzed by selection of electroactive substance introduced into the galvanic cell. The present invention enables a simple and easy analysis for food components such as glutamic acid, ascorbic acid, etc., water quality such as COD, etc.
Inventors
- 👤 Shunichi Uchiyama
- 👤 Takeshi Sato
- 👤 Hirofumi Akano
- 👤 Yoshiya Kawamura
- 👤 Masahiro Fukaya
- 👤 Hiroaki Ebisuya
- 👤 Ko Furukawa
- 👤 Kazuyo Kajino
- 👤 Sumio Akita
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 20:08
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/42; G01N27/423; G01N33/00; G01N33/18; G01N33/1806
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
122 words
A method for coulometric analysis for quantitative determination of a sample substance, comprising the steps of: providing a first electrode with a first substance having quinol formed from a reaction between the sample substance, which is a substrate of a quinone-dependent dehydrogenase, and quinone in the presence of quinone-dependent dehydrogenase, wherein the first substance is electroactive; providing a second electrode with a second substance, wherein the second substance is electroactive; providing an ion-permeable membrane between the first and second electrodes; measuring an electrical property generated by a potential difference from at least one reaction between the first and second substances without application of an external power source; and determining the quantity of said sample substance from the measure of the electrical property.
Claim 2
Independent Claim
122 words
A method for coulometric analysis for quantitative determination of a sample substance, comprising the steps of: providing a first electrode with a first substance having quinol formed from a reaction between the sample substance, which is a substrate of a quinone-dependent dehydrogenase, and quinone in the presence of quinone-dependent dehydrogenase, wherein the first substance is electroactive; providing a second electrode with a second substance, wherein the second substance is electroactive; providing an ion-permeable membrane between the first and second electrodes; measuring an electrical property generated by a potential difference from at least one reaction between the first and second substances without application of an external power source; and determining the quantity of said sample substance from the measure of the electrical property.
Claim 3
Dependent Claim
20 words
The method according to claim 1, wherein the sample substance is ethyl alcohol and the quinone-dependent dehydrogenase is alcohol dehydrogenase.
Claim 4
Dependent Claim
19 words
The method according to claim 1, wherein the sample substance is glucose and the quinone-dependent dehydrogenase is glucose dehydrogenase.
Claim 5
Dependent Claim
19 words
The method according to claim 1, wherein the sample substance is fructose and the quinone-dependent dehydrogenase is fructose dehydrogenase.
Claim 6
Dependent Claim
40 words
The method according to claim 1, wherein the sample substance consists of sucrose, glucose and fructose, and total sugar content of the sample substance is quantitatively determined by using quinone-dependent glucose dehydrogenase and fructose dehydrogenase in the presence of invertase.
Claim 7
Dependent Claim
18 words
The method according to claim 1, wherein the quinone is ubiquinone derivative represented by the following formula: ##STR7##
Claim 8
Independent Claim
116 words
A method for coulometric analysis for quantitative determination of a sample substance, comprising the steps of: providing a first electrode with a first substance having quinol formed from a reaction between the sample substance, which is a substrate of a quinone-dependent dehydrogenase, and quinone in the presence of quinone-dependent dehydrogenase, wherein the first substance is electroactive; providing a second electrode with a second substance, wherein the second substance is electroactive; providing an ion-permeable membrane between the first and second electrodes; measuring an electrical property generated by a potential difference from at least one self-driven reaction between the first and second substances; and determining the quantity of said sample substance from the measure of the electrical property.
Claim 9
Independent Claim
116 words
A method for coulometric analysis for quantitative determination of a sample substance, comprising the steps of: providing a first electrode with a first substance having quinol formed from a reaction between the sample substance, which is a substrate of a quinone-dependent dehydrogenase, and quinone in the presence of quinone-dependent dehydrogenase, wherein the first substance is electroactive; providing a second electrode with a second substance, wherein the second substance is electroactive; providing an ion-permeable membrane between the first and second electrodes; measuring an electrical property generated by a potential difference from at least one self-driven reaction between the first and second substances; and determining the quantity of said sample substance from the measure of the electrical property.
Claim 10
Dependent Claim
20 words
The method according to claim 7, wherein the sample substance is ethyl alcohol and the quinone-dependent dehydrogenase is alcohol dehydrogenase.
Claim 11
Dependent Claim
19 words
The method according to claim 7, wherein the sample substance is glucose and the quinone-dependent dehydrogenase is glucose dehydrogenase.
Claim 12
Dependent Claim
19 words
The method according to claim 7, wherein the sample substance is fructose and the quinone-dependent dehydrogenase is fructose dehydrogenase.
Claim 13
Dependent Claim
40 words
The method according to claim 7, wherein the sample substance consists of sucrose, glucose and fructose, and total sugar content of the sample substance is quantitatively determined by using quinone-dependent glucose dehydrogenase and fructose dehydrogenase in the presence of invertase.
Claim 14
Dependent Claim
18 words
The method according to claim 7, wherein the quinone is ubiquinone derivative represented by the following formula: ##STR8##
Claim 15
Independent Claim
121 words
An apparatus for coulometric analysis for quantitative determination of a sample substance, comprising: a first electrode with a first substance having quinol formed from a reaction between the sample substance, which is a substrate of a quinone-dependent dehydrogenase, and quinone in the presence of quinone-dependent dehydrogenase, wherein the first substance is electroactive; a second electrode with a second substance, wherein the second substance is electroactive; an ion-permeable membrane situated between the first and second electrodes; means for measuring an electrical property generated by a potential difference from at least one reaction between the first and second substances without application of an external power source; and means for determining the quantity of the sample substance from the measure of the electrical property.
Claim 16
Independent Claim
121 words
An apparatus for coulometric analysis for quantitative determination of a sample substance, comprising: a first electrode with a first substance having quinol formed from a reaction between the sample substance, which is a substrate of a quinone-dependent dehydrogenase, and quinone in the presence of quinone-dependent dehydrogenase, wherein the first substance is electroactive; a second electrode with a second substance, wherein the second substance is electroactive; an ion-permeable membrane situated between the first and second electrodes; means for measuring an electrical property generated by a potential difference from at least one reaction between the first and second substances without application of an external power source; and means for determining the quantity of the sample substance from the measure of the electrical property.
Claim 17
Dependent Claim
20 words
The apparatus according to claim 13, wherein the sample substance is ethyl alcohol and the quinone-dependent dehydrogenase is alcohol dehydrogenase.
Claim 18
Dependent Claim
19 words
The apparatus according to claim 13, wherein the sample substance is glucose and the quinone-dependent dehydrogenase is glucose dehydrogenase.
Claim 19
Dependent Claim
19 words
The apparatus according to claim 13, wherein the sample substance is fructose and the quinone-dependent dehydrogenase is fructose dehydrogenase.
Claim 20
Dependent Claim
40 words
The apparatus according to claim 13, wherein the sample substance consists of sucrose, glucose and fructose, and total sugar content of the sample substance is quantitatively determined by using quinone-dependent glucose dehydrogenase and fructose dehydrogenase in the presence of invertase.
Claim 21
Dependent Claim
18 words
The apparatus according to claim 13, wherein the quinone is ubiquinone derivative represented by the following formula: ##STR9##
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-03-09 | 2001-09-08 | 2002-03-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-03-09 | 2005-09-08 | 2006-03-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-03-09 | 2009-09-08 | 2010-03-10 | $7700.00 | Window Closed / Lapsed |