US5849174A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Electrodes and their use in analysis
Assignee: Medisense Inc
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Filing: 1995-08-01
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Granted: 1998-12-15
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Expires: 2015-08-01
Abstract
Abstract A method for indicating the concentration of a substance in solution includes passing an alternating voltage between a first electrode structure having coated thereon a polymer and a second separate counter electrode in the solution. The polymer is in one of an oxidized and a reduced state, between which states its conductivity varies. Changes in the conductivity of the polymer coating are measured, the measurement being representative of the state of the polymer and thereby of the concentration of the substance in the solution. Electrodes for use in this method are also described.
Inventors
- 👤 Gurdial Singh Sanghera
- 👤 Philip Nigel Bartlett
- 👤 Peter Robert Birkin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 11:04
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
106 words
A method for indicating the concentration of a substance in solution comprising passing an alternating voltage between a first electrode structure having coated thereon a polymer, the polymer being in one of an oxidised and a reduced state between which states its conductivity varies, and a second separate counter electrode in said solution; and measuring the change in conductivity of the polymer coating resulting from a chance in the state of the polymer due to a transfer of charge between the substance and the polymer, such measurement being representative of the state of the polymer and thereby of the concentration of said substance in the solution.
Claim 2
Independent Claim
106 words
A method for indicating the concentration of a substance in solution comprising passing an alternating voltage between a first electrode structure having coated thereon a polymer, the polymer being in one of an oxidised and a reduced state between which states its conductivity varies, and a second separate counter electrode in said solution; and measuring the change in conductivity of the polymer coating resulting from a chance in the state of the polymer due to a transfer of charge between the substance and the polymer, such measurement being representative of the state of the polymer and thereby of the concentration of said substance in the solution.
Claim 3
Dependent Claim
32 words
A method according to claim 1 wherein a first measurement is made in the absence of the substance in solution to provide a background reading of any interfering substances in said solution.
Claim 4
Dependent Claim
34 words
A method according to claim 1 or 2 wherein a substrate for an enzyme is immobilised on the polymer coating and the method is for indicating the concentration of the enzyme in the solution.
Claim 5
Dependent Claim
32 words
A method according to claim 1 wherein an enzyme is immobilised on the polymer coating, and the method is for indicating the concentration of a substrate for said enzyme in the solution.
Claim 6
Dependent Claim
30 words
A method according to claim 4 wherein said first electrode comprises a mediator associated with the polymer or enzyme coating, for transfer of charge between the substrate and the polymer.
Claim 7
Dependent Claim
33 words
A method according to claim 4 or claim 5 wherein a first measurement is made in the absence of said enzyme to provide a background reading of any interfering substances in said solution.
Claim 8
Dependent Claim
11 words
A method according to claim 1 wherein the polymer comprises poly(aniline).
Claim 9
Dependent Claim
21 words
A method according to claim 1 wherein the polymer film has a thickness in the range of 0.1 to 1 μm.
Claim 10
Dependent Claim
55 words
A method according to claim 1 wherein the first electrode structure comprises a plurality of electrodes each using a discrete polymer coating thereon, and an alternating voltage is passed through each electrode separately to measure the state of the conductivity of each, polymer coating and thereby the concentration of a plurality of substances in solution.
Claim 11
Dependent Claim
42 words
An electrode structure forming a single electrode in a circuit comprising an elongate electrode having an exposed face over which a polymer is coated, the polymer being in one of an oxidised and a reduced state between which states its conductivity varies.
Claim 12
Dependent Claim
42 words
An electrode structure forming a single electrode in a circuit comprising an elongate electrode having an exposed face over which a polymer is coated, the polymer being in one of an oxidised and a reduced state between which states its conductivity varies.
Claim 13
Dependent Claim
22 words
An electrode structure according to claim 10 wherein the polymer film is covered by a composition in which an enzyme is immobilised.
Claim 14
Dependent Claim
52 words
An electrode according to claim 10 wherein said electrode comprises a carbon microband electrode having one or more carbon electrodes inter-layered with insulating dielectric layers printed on a substrate, each carbon electrode having an exposed end of carbon, the polymer being coated on the exposed ends of carbon of said microband electrode.
Claim 15
Dependent Claim
21 words
An electrode according to claim 10 wherein said polymer film has a thickness in the range of 0.1 to 1 μm.
Claim 16
Dependent Claim
21 words
An electrode structure according to claim 10 and comprising a plurality of electrodes each having coated thereon a discrete polymer film.
Claim 17
Dependent Claim
19 words
An electrode structure according to claim 14 wherein an enzyme is overcoated onto one of the discrete polymer films.
Claim 18
Dependent Claim
63 words
An electrode structure forming a single electrode in a circuit comprising a carbon microband electrode having one or more carbon electrodes inter-layered with insulating dielectric layers printed on a substrate, each carbon electrode having an exposed end of carbon over which a polymer is coated, the polymer being in one of an oxidised and a reduced state between which states its conductivity varies.
Claim 19
Dependent Claim
63 words
An electrode structure forming a single electrode in a circuit comprising a carbon microband electrode having one or more carbon electrodes inter-layered with insulating dielectric layers printed on a substrate, each carbon electrode having an exposed end of carbon over which a polymer is coated, the polymer being in one of an oxidised and a reduced state between which states its conductivity varies.
Claim 20
Dependent Claim
21 words
An electrode according to claim 16 wherein said polymer film has a thickness in the range of 0.1 to 1 μm.
Claim 21
Dependent Claim
21 words
An electrode structure according to claim 16 and comprising a plurality of electrodes each having coated thereon a discrete polymer film.
Claim 22
Dependent Claim
19 words
An electrode structure according to claim 16 wherein an enzyme is overcoated onto one of the discrete polymer films.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-12-14 | 2002-06-15 | 2002-12-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-12-14 | 2006-06-15 | 2006-12-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-12-14 | 2010-06-15 | 2010-12-15 | $7700.00 | Window Closed / Lapsed |