US5866349A
Expired / Lapsed
US
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Method for determination of glucose in whole blood and cuvette and photometer for carrying out said method
Assignee: Individual
•
Filing: 1990-04-24
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Granted: 1999-02-02
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Expires: 2010-04-24
Abstract
Abstract A sample of whole blood is contacted with a reagent which by chemical reaction with glucose in the sample brings about a detectable dye concentration change (10) the size of which is determined as a measure of the glucose content of the sample. The sample is initially introduced undiluted in a microcuvette having at least one cavity for receiving the sample. The cavity is internally pretreated with the reagent in dry form, and the chemical reaction takes place in the cavity. Active components of the reagent comprise at least a hemolyzing agent for exposing glucose contained in the blood cells of the sample for allowing total glucose determination, and agents taking part in the chemical reaction and ensuring that the dye concentration change (10) takes place at least in a wavelength range (14) outside the absorption range (12) of the blood hemoglobin. An absorption measurement is performed in said wavelength range directly on the sample in the cuvette. A pretreated disposable cuvette with such a reagent and a photometer are also described.
Inventors
- 👤 Jan Evert Lilja
- 👤 Sven-Erik Lennart Nilsson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 03:06
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; G; G01; G01N; G01N21/00; G01N21/01; G01N21/03; G01N21/17; G01N21/25; G01N21/31; G01N21/314; G01N15/00; G01N15/01; G01N2015/011; G01N2021/0321; G01N2021/0325; G01N2021/0346; G01N2021/3181; G01N21/75; G01N21/77; G01N21/78; G01N2201/00; G01N2201/12; G01N2201/122; G01N2201/1222; G01N2201/126; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/493
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
198 words
A method for determining the glucose content in whole blood, in which a sample of undiluted whole blood is contacted with a dye-containing reagent system which undergoes chemical reaction with the glucose in the whole blood sample, the method consisting essentially of the steps of: introducing the undiluted whole blood sample in a microcuvette having at least one cavity for receiving the sample, said cavity being internally pretreated with the reagent in a dry form and said chemical reaction then taking place in said cavity, the reagent being comprised of a hemolyzing agent and agents used in the glucose dehydrogenase method, said agents being comprised of glucose dehydrogenase and a redox indicator dye, the hemolyzing agent exposing the glucose contained in the blood cells of the whole blood sample permitting a quantitative total glucose determination in a whole blood hemolysate, the agents which participate in the chemical reaction ensure that a dye concentration change takes place in a wavelength range above 650 nm and, performing an absorption measurement at said wavelength range directly on the sample in the cuvette, and further conducting a secondary absorption measurement to compensate for background interference in a wavelength range above 700 nm.
Claim 2
Independent Claim
198 words
A method for determining the glucose content in whole blood, in which a sample of undiluted whole blood is contacted with a dye-containing reagent system which undergoes chemical reaction with the glucose in the whole blood sample, the method consisting essentially of the steps of: introducing the undiluted whole blood sample in a microcuvette having at least one cavity for receiving the sample, said cavity being internally pretreated with the reagent in a dry form and said chemical reaction then taking place in said cavity, the reagent being comprised of a hemolyzing agent and agents used in the glucose dehydrogenase method, said agents being comprised of glucose dehydrogenase and a redox indicator dye, the hemolyzing agent exposing the glucose contained in the blood cells of the whole blood sample permitting a quantitative total glucose determination in a whole blood hemolysate, the agents which participate in the chemical reaction ensure that a dye concentration change takes place in a wavelength range above 650 nm and, performing an absorption measurement at said wavelength range directly on the sample in the cuvette, and further conducting a secondary absorption measurement to compensate for background interference in a wavelength range above 700 nm.
Claim 3
Dependent Claim
15 words
The method as claimed in claim 1, wherein the reagent is further comprised of diaphorase.
Claim 4
Dependent Claim
15 words
The method as claimed in claim 2 wherein the reagent is further comprised of mutarotase.
Claim 5
Dependent Claim
30 words
The method as claimed in claim 1 wherein said chemical reaction is an end-point reaction, said absorption measurement being carried out only when said dye concentration change is substantially terminated.
Claim 6
Dependent Claim
191 words
A disposable cuvette for carrying out a determination of the glucose content of undiluted whole blood where a sample of undiluted whole blood is contacted with a dye-containing reagent system undergoes chemical reaction with the glucose in the undiluted whole blood sample, the cuvette being comprised of at least one cavity for receiving the undiluted whole blood sample, said cavity being internally pretreated with a reagent in dry form and said chemical reaction occurring in said cavity after introduction of the sample in undiluted form, the reagent being comprised of a hemolyzing agent and agents used in the glucose dehydrogenase method, said agents being comprised of glucose dehydrogenase and a redox indicator dye, the hemolyzing agent exposing the glucose contained in the blood cells of the whole blood sample permitting a quantitative total glucose determination in a whole blood hemolysate, the agents which participate in the chemical reaction ensure that the dye concentration change takes place in a wavelength range above 650 nm and that the cuvette is at least partly transparent for permitting an absorption measurement directly on the sample in the cavity of the cuvette in said wavelength range.
Claim 7
Dependent Claim
191 words
A disposable cuvette for carrying out a determination of the glucose content of undiluted whole blood where a sample of undiluted whole blood is contacted with a dye-containing reagent system undergoes chemical reaction with the glucose in the undiluted whole blood sample, the cuvette being comprised of at least one cavity for receiving the undiluted whole blood sample, said cavity being internally pretreated with a reagent in dry form and said chemical reaction occurring in said cavity after introduction of the sample in undiluted form, the reagent being comprised of a hemolyzing agent and agents used in the glucose dehydrogenase method, said agents being comprised of glucose dehydrogenase and a redox indicator dye, the hemolyzing agent exposing the glucose contained in the blood cells of the whole blood sample permitting a quantitative total glucose determination in a whole blood hemolysate, the agents which participate in the chemical reaction ensure that the dye concentration change takes place in a wavelength range above 650 nm and that the cuvette is at least partly transparent for permitting an absorption measurement directly on the sample in the cavity of the cuvette in said wavelength range.
Claim 8
Independent Claim
257 words
A method for determining the glucose content in undiluted whole blood, in which a sample of undiluted whole blood is contacted with a reagent which by chemical reaction with glucose in the undiluted whole blood sample brings about a dye concentration change which is detectable in the sample, whereby the size of the dye concentration change is used to determine the measure of the glucose content, the method consisting essentially of the steps of: introducing a sample of undiluted whole blood in a microcuvette having at least one cavity for receiving the sample, said cavity being internally pretreated with a reagent in dry form and said chemical reaction with the glucose in the sample taking place in said cavity, the reagent being comprised of a hemolyzing agent for exposing glucose contained in the blood cells of the sample for allowing a quantitative total glucose determination in a whole blood hemolysate, glucose dehydrogenase, NAD or analog thereof, diaphorase or analog thereof, and a dye, the dye undergoing a concentration change upon said chemical reaction with the reagent corresponding to the blood glucose content of the sample, determining by transmission photometry the concentration change of the dye at a wavelength that is in an absorption range above 650 nm., the dye being selected so that the dye concentration change takes place at least in a wavelength range above 650 nm. which is outside the absorption range of the blood hemoglobin, and further conducting a secondary absorption measurement to compensate for background interference in a wavelength range above 700 nm.
Claim 9
Independent Claim
257 words
A method for determining the glucose content in undiluted whole blood, in which a sample of undiluted whole blood is contacted with a reagent which by chemical reaction with glucose in the undiluted whole blood sample brings about a dye concentration change which is detectable in the sample, whereby the size of the dye concentration change is used to determine the measure of the glucose content, the method consisting essentially of the steps of: introducing a sample of undiluted whole blood in a microcuvette having at least one cavity for receiving the sample, said cavity being internally pretreated with a reagent in dry form and said chemical reaction with the glucose in the sample taking place in said cavity, the reagent being comprised of a hemolyzing agent for exposing glucose contained in the blood cells of the sample for allowing a quantitative total glucose determination in a whole blood hemolysate, glucose dehydrogenase, NAD or analog thereof, diaphorase or analog thereof, and a dye, the dye undergoing a concentration change upon said chemical reaction with the reagent corresponding to the blood glucose content of the sample, determining by transmission photometry the concentration change of the dye at a wavelength that is in an absorption range above 650 nm., the dye being selected so that the dye concentration change takes place at least in a wavelength range above 650 nm. which is outside the absorption range of the blood hemoglobin, and further conducting a secondary absorption measurement to compensate for background interference in a wavelength range above 700 nm.
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