US6015715A
Expired / Lapsed
US
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Method of manufacturing a sensitive single-layer system for measuring the concentration of analytes, and a system produced by this method
Assignee: Individual
•
Filing: 1996-05-24
•
Granted: 2000-01-18
•
Expires: 2016-05-24
Abstract
Abstract Method of manufacturing a sensitive single-layer system for optically detecting a concentration of an analyte, such as, for example, oxygen, the analyte being capable of investigation in liquid or gaseous aggregate condition or in a dissolved condition, by adsorption, luminescence or luminescence quenching of an indicator sensitive to the analyte, which is preferably permanently immobilized in the optical beam path. The sensitive single-layer system achieves a reproducible and extremely short response behavior. Indicators are adsorbed on a filler material and thereafter a mixture is produced with a matrix material permeable to the analyte to be detected. Then, under the action of pressure, the mixture is extruded onto a substrate, the layer thickness being formed in dependence on the applied pressure used. The applied sensitive layer is polymerized, polycondensed or hardened and the layer is also homogenized by swelling in a fluorescence indicator solution.
Inventors
- 👤 Uwe Kirschner
- 👤 Matthias Lau
- 👤 Birgit Hannemann
- 👤 Adriana Tamachkiarowa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 16:19
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/75; G01N21/77; G01N21/78; G01N21/783
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
86 words
Method of manufacturing a single-layer system for measuring concentration of analytes, comprising: adsorbing fluorescence indicator on filler material forming fluorescence indicator adsorbed filler material; mixing the fluorescence indicator adsorbed filler material with a permeable matrix material forming a mixture; applying the mixture under a pressure of 12 to 20×10 4 Pa onto a substrate to obtain a sensitive layer having a thickness and shape; polymerizing, polycondensing or hardening the matrix material; and homogenizing the layer by swelling with a solvent capable of dissolving the fluorescence indicator.
Claim 2
Dependent Claim
86 words
Method of manufacturing a single-layer system for measuring concentration of analytes, comprising: adsorbing fluorescence indicator on filler material forming fluorescence indicator adsorbed filler material; mixing the fluorescence indicator adsorbed filler material with a permeable matrix material forming a mixture; applying the mixture under a pressure of 12 to 20×10 4 Pa onto a substrate to obtain a sensitive layer having a thickness and shape; polymerizing, polycondensing or hardening the matrix material; and homogenizing the layer by swelling with a solvent capable of dissolving the fluorescence indicator.
Claim 3
Dependent Claim
27 words
The method according to claim 1, wherein the applying comprises extruding the mixture into a mold to obtain the sensitive layer on a position on the substrate.
Claim 4
Dependent Claim
12 words
The method according to claim 1, wherein the permeable material comprises polydimethylsiloxane.
Claim 5
Dependent Claim
12 words
The method according to claim 1, wherein the solvent comprises methylene chloride.
Claim 6
Dependent Claim
23 words
The method according to claim 1, wherein the filler material comprises member selected from the group consisting of silica gel and porous glass.
Claim 7
Dependent Claim
16 words
The method according to claim 1, wherein the applying under pressure comprises compressing in a mold.
Claim 8
Dependent Claim
13 words
The method according to claim 1, wherein the matrix material comprises a polysiloxane.
Claim 9
Dependent Claim
13 words
The method according to claim 1, wherein the substrate comprises a reflective substrate.
Claim 10
Dependent Claim
13 words
The method according to claim 1, wherein the substrate comprises a reflective substrate.
Claim 11
Dependent Claim
37 words
The method according to claim 1, wherein the substrate comprises a member selected from the group consisting of an optical fiber, a bundle of optical fibers, a plate, a lens, a prism and an integrated optical structure.
Claim 12
Dependent Claim
11 words
Sensitive-single layer system produced by the method according to claim 1.
Claim 13
Dependent Claim
19 words
The method according to claim 1, wherein the applying under pressure comprises utilizing a pressure of 15×10 4 Pa.
Claim 14
Dependent Claim
11 words
Sensitive-single layer system produced by the method according to claim 12.
Claim 15
Dependent Claim
89 words
The method according to claim 1, wherein the permeable material comprises a polysiloxane; the solvent comprises a solution containing the fluorescence indicator in a molar concentration of 10 -1 to 10 -6 ; the layer includes an overall concentration of fluorescence indicator in the matrix of 10 -1 to 10 -6 mol/l; the filler material comprises a member selected from the group consisting of silica gel and porous glass; and the filler material is contained in the matrix material with a weight proportion of 5 to 65% by weight.
Claim 16
Dependent Claim
11 words
Sensitive-single layer system produced by the method according to claim 14.
Claim 17
Dependent Claim
14 words
The method according to claim 1, wherein the fluorescence indicator comprises a ruthenium-diamine complex.
Claim 18
Dependent Claim
11 words
Sensitive-single layer system produced by the method according to claim 16.
Claim 19
Dependent Claim
27 words
The method according to claim 1, wherein the solvent comprises a solution containing the fluorescence indicator in a molar concentration of 10 -1 to 10 -6 .
Claim 20
Dependent Claim
27 words
The method according to claim 18, wherein the solvent comprises a solution containing the fluorescence indicator in a molar concentration of 10 -3 to 10 -4 .
Claim 21
Dependent Claim
14 words
The method according to claim 18, wherein the solution comprises a methylene chloride solution.
Claim 22
Dependent Claim
11 words
Sensitive-single layer system produced by the method according to claim 18.
Claim 23
Dependent Claim
26 words
The method according to claim 1, wherein the layer includes an overall concentration of fluorescence indicator in the matrix of 10 -1 to 10 -6 mol/l.
Claim 24
Dependent Claim
26 words
The method according to claim 22, wherein the layer includes an overall concentration of fluorescence indicator in the matrix of 10 -2 to 10 -4 mol/l.
Claim 25
Dependent Claim
11 words
Sensitive-single layer system produced by the method according to claim 22.
Claim 26
Dependent Claim
26 words
The method according to claim 1, wherein the filler material is contained in the matrix material with a weight proportion of 5 to 65% by weight.
Claim 27
Dependent Claim
26 words
The method according to claim 25, wherein the filler material is contained in the matrix material with a weight proportion of 20 to 30% by weight.
Claim 28
Dependent Claim
13 words
The method according to claim 25, wherein said filler material comprises silica gel.
Claim 29
Dependent Claim
13 words
The method according to claim 25, wherein said filler material comprised porous glass.
Claim 30
Dependent Claim
92 words
Method of manufacturing a single-layer system for measuring concentration of analytes, comprising: adsorbing fluorescence indicator on filler material forming fluorescence indicator adsorbed filler material; mixing the fluorescence indicator adsorbed filler material with a permeable matrix material forming a mixture; applying the mixture under pressure onto a substrate to obtain a sensitive layer having a thickness and shape; polymerizing, polycondensing or hardening the matrix material; homogenizing the layer by swelling with a solvent capable of dissolving the fluorescence indicator; and wherein the substrate comprises a lens, and said lens comprises a GRIN lens.
Claim 31
Dependent Claim
92 words
Method of manufacturing a single-layer system for measuring concentration of analytes, comprising: adsorbing fluorescence indicator on filler material forming fluorescence indicator adsorbed filler material; mixing the fluorescence indicator adsorbed filler material with a permeable matrix material forming a mixture; applying the mixture under pressure onto a substrate to obtain a sensitive layer having a thickness and shape; polymerizing, polycondensing or hardening the matrix material; homogenizing the layer by swelling with a solvent capable of dissolving the fluorescence indicator; and wherein the substrate comprises a lens, and said lens comprises a GRIN lens.
Claim 32
Dependent Claim
82 words
Method of manufacturing a single-layer system for measuring concentration of analytes, comprising: adsorbing indicator on filler material forming indicator adsorbed filler material; mixing the indicator adsorbed filler material with a permeable matrix material forming a mixture; applying the mixture under a pressure of 12 to 20×10 4 Pa onto a substrate to obtain a sensitive layer having a thickness and shape; polymerizing, polycondensing or hardening the matrix material; and homogenizing the layer by swelling with a solvent capable of dissolving the indicator.
Claim 33
Dependent Claim
82 words
Method of manufacturing a single-layer system for measuring concentration of analytes, comprising: adsorbing indicator on filler material forming indicator adsorbed filler material; mixing the indicator adsorbed filler material with a permeable matrix material forming a mixture; applying the mixture under a pressure of 12 to 20×10 4 Pa onto a substrate to obtain a sensitive layer having a thickness and shape; polymerizing, polycondensing or hardening the matrix material; and homogenizing the layer by swelling with a solvent capable of dissolving the indicator.
Claim 34
Dependent Claim
11 words
Sensitive-single layer system produced by the method according to claim 30.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-01-17 | 2003-07-19 | 2004-01-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-01-17 | 2007-07-19 | 2008-01-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-01-17 | 2011-07-19 | 2012-01-18 | $7700.00 | Window Closed / Lapsed |