US6143293A
Expired / Lapsed
US
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Assembled scaffolds for three dimensional cell culturing and tissue generation
Assignee: LifeCell Corp
•
Filing: 1998-03-26
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Granted: 2000-11-07
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Expires: 2018-03-26
Abstract
Abstract A three-dimensional scaffold for tissue generation. Mechanical fasteners allow layered and volumetric scaffold sections, which may be pre-seeded with cells and/or growth factors, to be assembled into a heterogeneous generated tissue for implantation.
Inventors
- 👤 Lee E. Weiss
- 👤 Jay Wynn Calvert
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 10:25
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61L; A61L27/00; A61L27/14; A61L27/18; A61L27/36; A61L27/3604; A61L27/3625; A61L27/38; C; C12; C12N; C12N5/00; C12N5/0062; A61F; A61F2/00; A61F2/0059; A61F2/02; A61F2/022; A61L2430/00; A61L2430/22
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
34 words
A three dimensional multilayer scaffold for supporting cell growth comprising: a first layer for supporting cell growth; and a second layer for supporting cell growth, said first and second layers being mechanically fastened together.
Claim 2
Dependent Claim
34 words
A three dimensional multilayer scaffold for supporting cell growth comprising: a first layer for supporting cell growth; and a second layer for supporting cell growth, said first and second layers being mechanically fastened together.
Claim 3
Dependent Claim
37 words
The three dimensional multilayer scaffold of claim 1, wherein said first and second layers are mechanically fastened together by a mechanical fastener selected from the group consisting of screws, pins, sutures, staples, wires, string and combinations thereof.
Claim 4
Dependent Claim
14 words
The three dimensional multilayer scaffold of claim 2, wherein said mechanical fastener is bioabsorbable.
Claim 5
Dependent Claim
15 words
The three dimensional multilayer scaffold of claim 1, wherein said first layer includes a channel.
Claim 6
Dependent Claim
46 words
The three dimensional multilayer scaffold of claim 1, wherein each of said layers includes an alignment indentation on one surface thereof and an alignment protrusion on an opposing surface thereof, the alignment indentations shaped to mate with the alignment protrusions for facile alignment of said layers.
Claim 7
Dependent Claim
38 words
The three dimensional multilayer scaffold of claim 1, wherein said first layer is seeded with a component selected from the group consisting of living cells, bone marrow cells, osteoblasts, mesenchymal stem cells, satellite cells, fibroblasts, and growth factors.
Claim 8
Dependent Claim
38 words
The three dimensional multilayer scaffold of claim 6, wherein said second layer is seeded with a component selected from the group consisting of living cells, bone marrow cells, osteoblasts, mesenchymal stem cells, satellite cells, fibroblasts, and growth factors.
Claim 9
Dependent Claim
24 words
The three dimensional multilayer scaffold of claim 1, wherein said first and second layers are subdivided into zones of support for a pre-selected component.
Claim 10
Dependent Claim
28 words
The three dimensional multilayer scaffold of claim 8, wherein said zones of support for a pre-selected component define three dimensional heterogeneous regions in the three dimensional multilayer scaffold.
Claim 11
Dependent Claim
15 words
The three dimensional multilayer scaffold of claim 1, further including a blood vessel embedded therein.
Claim 12
Dependent Claim
20 words
The three dimensional multilayer scaffold of claim 10, further including a barbed halo positioned around said blood vessel's outer surface.
Claim 13
Independent Claim
87 words
A method of forming a three dimensional multilayer scaffold comprising: seeding a first prefabricated element with a component selected from the group consisting of living cells, bone marrow cells, osteoblasts, mesenchymal stem cells, satellite cells, fibroblasts, and growth factors; seeding a second prefabricated element with a component selected from the group consisting of living cells, bone marrow cells, osteoblasts, mesenchymal stem cells, satellite cells, fibroblasts, and growth factors; and connecting said first and second prefabricated elements together in a manner that ensures viability of said cellular components.
Claim 14
Independent Claim
87 words
A method of forming a three dimensional multilayer scaffold comprising: seeding a first prefabricated element with a component selected from the group consisting of living cells, bone marrow cells, osteoblasts, mesenchymal stem cells, satellite cells, fibroblasts, and growth factors; seeding a second prefabricated element with a component selected from the group consisting of living cells, bone marrow cells, osteoblasts, mesenchymal stem cells, satellite cells, fibroblasts, and growth factors; and connecting said first and second prefabricated elements together in a manner that ensures viability of said cellular components.
Claim 15
Dependent Claim
16 words
The method of claim 12, wherein a mechanical fastener connects said first and second prefabricated elements.
Claim 16
Independent Claim
56 words
A method of treating a patient comprising: constructing a three dimensional scaffold to support cell growth, said three dimensional scaffold including a first layer for supporting cell growth, and a second layer for supporting cell growth, said first and second layers being mechanically connected to each other; and implanting said three dimensional scaffold in the patient.
Claim 17
Independent Claim
56 words
A method of treating a patient comprising: constructing a three dimensional scaffold to support cell growth, said three dimensional scaffold including a first layer for supporting cell growth, and a second layer for supporting cell growth, said first and second layers being mechanically connected to each other; and implanting said three dimensional scaffold in the patient.
Claim 18
Dependent Claim
34 words
The method of claim 14, wherein said first layer is pre-seeded with a cellular component selected from the group consisting of bone marrow cells, osteoblasts, mesenchymal stem cells, satellite cells, fibroblasts, and growth factors.
Claim 19
Dependent Claim
34 words
The method of claim 15, wherein said second layer is pre-seeded with a cellular component selected from the group consisting of bone marrow cells, osteoblasts, mesenchymal stem cells, satellite cells, fibroblasts, and growth factors.
Claim 20
Dependent Claim
25 words
The method of claim 14, wherein said three dimensional scaffold further includes a plurality of other layers mechanically connected to said first and second layers.
Claim 21
Dependent Claim
18 words
The method of claim 14, further including the step of embedding a vessel in said three dimensional scaffold.
Claim 22
Dependent Claim
17 words
The method of claim 18, further including the step of connecting the vessel to an existing vessel.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-11-07 | 2004-05-08 | 2004-11-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-11-07 | 2008-05-08 | 2008-11-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-11-07 | 2012-05-08 | 2012-11-07 | $7700.00 | Window Closed / Lapsed |