US6147203A
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Recombinant disulfide-stabilized polypeptide fragments having binding specificity
Assignee: United States Department of Health and Human Services
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Filing: 1998-01-05
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Granted: 2000-11-14
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Expires: 2018-01-05
Abstract
Abstract The present invention relates to disulfide-stabilized recombinant polypeptide molecules which have the binding ability and specificity for another peptide, such as the variable region of an antibody molecule. Methods of producing these molecules and nucleic acid sequences encoding these molecules are also described. In particular, the invention discloses Fv antibody fragments stabilized by a disulfide bond connecting the VH and VL regions of the Fv fragment. The alpha and beta chains of T cell receptors may be similarly stabilized by means described in the invention.
Inventors
- 👤 Ira H. Pastan
- 👤 Byungkook Lee
- 👤 Sun-Hee Jung
- 👤 Ulrich Brinkmann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 05:52
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/705; C07K14/70503; C07K14/7051; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/68; A61K47/6801; A61K47/6803; A61K47/6811; A61K47/6817; A61K47/6829; A61K47/6835; A61P; A61P35/00; C07K16/00; A61K38/00; C07K2319/00; C07K2319/33; C07K2319/55; Y; Y10; Y10S; Y10S530/00; Y10S530/866; Y10S530/867
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
50 words
A nucleic acid that encodes a light chain variable region (V L ) of an antibody wherein the V L contains a cysteine at position 42, 43, 44, 45, 46, 98, 99, 100, or 101, such positions being determined in accordance with the numbering scheme published by Kabat and Wu.
Claim 2
Dependent Claim
50 words
A nucleic acid that encodes a light chain variable region (V L ) of an antibody wherein the V L contains a cysteine at position 42, 43, 44, 45, 46, 98, 99, 100, or 101, such positions being determined in accordance with the numbering scheme published by Kabat and Wu.
Claim 3
Dependent Claim
18 words
A nucleic acid of claim 1, which encodes a cysteine at position 100 of the V L .
Claim 4
Dependent Claim
18 words
A nucleic acid of claim 1, which encodes a cysteine at position 43 of the V L .
Claim 5
Dependent Claim
50 words
A nucleic acid that encodes a heavy chain variable region (V H ) of an antibody wherein the V H contains a cysteine at position 43, 44, 45, 46, 47, 103, 104, 105 or 106, such positions being determined in accordance with the numbering scheme published by Kabat and Wu.
Claim 6
Dependent Claim
50 words
A nucleic acid that encodes a heavy chain variable region (V H ) of an antibody wherein the V H contains a cysteine at position 43, 44, 45, 46, 47, 103, 104, 105 or 106, such positions being determined in accordance with the numbering scheme published by Kabat and Wu.
Claim 7
Dependent Claim
18 words
A nucleic acid of claim 4, which encodes a cysteine at position 4 of the V H .
Claim 8
Dependent Claim
18 words
A nucleic acid of claim 4, which encodes a cysteine at position 105 of the V H .
Claim 9
Dependent Claim
19 words
The nucleic acid of claim 1 which further includes a nucleic acid that encodes a toxin or pharmaceutical agent.
Claim 10
Dependent Claim
22 words
The nucleic acid sequence of claim 7, wherein the toxin or pharmaceutical agent is connected to the antibody by a peptide linker.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-11-14 | 2004-05-15 | 2004-11-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-11-14 | 2008-05-15 | 2008-11-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-11-14 | 2012-05-15 | 2012-11-14 | $7700.00 | Window Closed / Lapsed |