US4946778A
Expired / Lapsed
US
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Single polypeptide chain binding molecules
Assignee: Genex Corp
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Filing: 1989-01-19
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Granted: 1990-08-07
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Expires: 2009-01-19
Abstract
Abstract The invention pertains to a single polypeptide chain binding molecule which has binding specificity and affinity substantially similar to the binding specificity and affinity of the light and heavy chain aggregate variable region of an antibody, to genetic sequences coding therefor, and to recombinant DNA methods of producing such molecule and uses for such molecule.
Inventors
- 👤 Robert C. Ladner
- 👤 Robert E. Bird
- 👤 Karl Hardman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 18:23
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/46; C07K16/468; C07K16/18; C07K16/28; C07K16/30; C07K2319/00; C07K2319/01; C07K2319/036; Y; Y10; Y10S; Y10S530/00; Y10S530/861; Y10S530/866; Y10S530/867
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
80 words
A genetic sequence which codes for a single chain polypeptide having binding affinity for an antigen, said polypeptide comprising: (a) a first polypeptide comprising the binding portion of the light chain variable region of an antibody; (b) a second polypeptide comprising the binding portion of the heavy chain variable region of an antibody; and (c) at least one peptide linker linking said first and second polypeptides (a) and (b) into a single chain polypeptide having binding affinity for said antigen.
Claim 2
Dependent Claim
80 words
A genetic sequence which codes for a single chain polypeptide having binding affinity for an antigen, said polypeptide comprising: (a) a first polypeptide comprising the binding portion of the light chain variable region of an antibody; (b) a second polypeptide comprising the binding portion of the heavy chain variable region of an antibody; and (c) at least one peptide linker linking said first and second polypeptides (a) and (b) into a single chain polypeptide having binding affinity for said antigen.
Claim 3
Dependent Claim
16 words
The genetic sequence of claim 1 wherein said peptide linker (c) is not from an antibody.
Claim 4
Dependent Claim
26 words
The sequence of claim 1 wherein said single chain polypeptide comprises one linker linking said first and second polypeptides (a) and (b) into said single chain.
Claim 5
Dependent Claim
44 words
The sequence of claim 3 wherein said single chain polypeptide comprises in sequence: (i) an N-terminal polypeptide from the light chain variable region of an antibody; (ii) a peptide linker; and (iii) a C-terminal polypeptide from the heavy chain variable region of an antibody.
Claim 6
Dependent Claim
44 words
The sequence of claim 3 wherein said single chain polypeptide comprises in sequence: (i) an N-terminal polypeptide from the heavy chain variable region of an antibody; (ii) a peptide linker; and (iii) a C-terminal polypeptide from the light chain variable region of an antibody.
Claim 7
Dependent Claim
24 words
The sequence of claim 1 wherein said single chain polypeptide comprises two peptide linkers linking said first and second polypeptides into said single chain.
Claim 8
Dependent Claim
60 words
The sequence of claim 6 wherein said single chain polypeptide comprises, in sequence: (i) an N-terminal polypeptide from the light chain variable region of an antibody; (ii) a peptide linker; (iii) a polypeptide from the heavy chain variable region of an antibody; (iv) a peptide linker; and (v) a C-terminal polypeptide from the light chain variable region of an antibody.
Claim 9
Dependent Claim
23 words
The sequence of claim 4, 5 or 7 wherein said single chain polypeptide comprises, prior to said N-terminal region (i), a methionine residue.
Claim 10
Dependent Claim
21 words
A replicable cloning or expression vehicle comprising the sequence of any of claims 1, 2, 3, 4, 5, 6, or 7.
Claim 11
Dependent Claim
9 words
The vehicle of claim 9 which is a plasmid.
Claim 12
Dependent Claim
10 words
A host cell transformed with the vehicle of claim 9.
Claim 13
Dependent Claim
23 words
The host cell of claim 11 which is a bacterial cell, a yeast cell or other fungal cell, or a mammalian cell line.
Claim 14
Dependent Claim
13 words
The host cell of claim 12 which is E. coli or Bacillus subtilis.
Claim 15
Independent Claim
111 words
A method of producing a single chain polypeptide having binding affinity for an antigen, said polypeptide comprising: (a) a first polypeptide comprising the binding portion of the light chain variable region of an antibody; (b) a second polypeptide comprising the binding portion of the heavy chain variable region of an antibody; and (c) a peptide linker linking said first and second polypeptides (a) and (b) into said single chain polypeptide, said method comprising: (i) providing a genetic sequence coding for said single chain polypeptide; (ii) transforming a host cell with said sequence; (iii) expressing said sequence in said host; (iv) recovering said single chain polypeptide having binding affinity for an antigen.
Claim 16
Independent Claim
111 words
A method of producing a single chain polypeptide having binding affinity for an antigen, said polypeptide comprising: (a) a first polypeptide comprising the binding portion of the light chain variable region of an antibody; (b) a second polypeptide comprising the binding portion of the heavy chain variable region of an antibody; and (c) a peptide linker linking said first and second polypeptides (a) and (b) into said single chain polypeptide, said method comprising: (i) providing a genetic sequence coding for said single chain polypeptide; (ii) transforming a host cell with said sequence; (iii) expressing said sequence in said host; (iv) recovering said single chain polypeptide having binding affinity for an antigen.
Claim 17
Dependent Claim
20 words
The method of claim 14 which further comprises purifying said recovered single chain polypeptide having binding affinity for an antigen.
Claim 18
Dependent Claim
23 words
The method of claim 14 wherein said host cell is a bacterial cell, yeast or other fungal cell or a mammalian cell line.
Claim 19
Dependent Claim
15 words
The method of claim 16 wherein said host cell is E. coli or Bacillus subtilis.
Claim 20
Dependent Claim
33 words
The genetic sequence of claim 1 wherein said first polypeptide (a) comprises substantially all of the light chain variable region, and said polypeptide (b) comprises substantially all of the heavy chain variable region.
Claim 21
Dependent Claim
32 words
The method of claim 14 wherein said first polypeptide (a) comprises substantially all of the light chain variable region, and said polypeptide (b) comprises substantially all of the heavy chain variable region.
Claim 22
Dependent Claim
59 words
The method of claim 14 which comprises prior to said step (i), the following: (v) operably linking a genetic sequence coding for said first polypeptide (a) to a genetic sequence coding for said first polypeptide (b) to a genetic sequence coding for said peptide linker (c); so as to provide said genetic sequence coding for said single chain polypeptide.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-08-06 | 1994-02-05 | 1994-08-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-08-06 | 1998-02-05 | 1998-08-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-08-06 | 2002-02-05 | 2002-08-07 | $7700.00 | Window Closed / Lapsed |