US6506575B1
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US
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Analytical element and method for the determination of an analyte in a liquid
Assignee: Roche Diagnostics GmbH
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Filing: 2000-09-22
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Granted: 2003-01-14
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Expires: 2020-09-22
Abstract
Abstract The invention concerns an analytical element for determining the amount of an analyte in a liquid containing a sample application zone (1) and a detection zone (2) which are in liquid-transferring contact, the latter containing at least one enzyme and an indicator substance, the enzyme catalysing a reaction in which the analyte or a substance derived from the analyte participates and the indicator substance forming a signal when the analyte is present which signal correlates with the amount of analyte, and containing a liquid-permeable interference-reducing layer (3) in direct contact with the detection zone (2) arranged such that liquid does not reach the detection zone until it has passed through the interference-reducing layer, the interference-reducing layer containing at least one enzyme which participates in a reaction of the analyte to be determined or of a substance derived from the analyte, wherein the analyte or the substance derived from the analyte is converted enzymatically in the interference-reducing layer into end products which cannot contribute to the signal formation by the indicator substance, and a method for the determination of an analyte in a liquid by means of such an analytical element.
Inventors
- 👤 Wolfgang Reinhold Knappe
- 👤 Rudolf Pachl
- 👤 Otto Gaa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 23:07
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
166 words
Analytical element for determining the amount of an analyte in a liquid, the element comprising a sample application zone and a detection zone which are in liquid-transferring contact, the detection zone containing at least one enzyme and an indicator substance, the enzyme catalysing a reaction in which the analyte or a substance derived from the analyte participates and the indicator substance forming a signal when the analyte is present which signal correlates with the amount of analyte, and a liquid-permeable interference-reducing layer in direct contact with the detection zone arranged such that liquid does not reach the detection zone until the liquid has passed through the interference-reducing layer, the interference-reducing layer containing at least one enzyme which participates in a reaction of the analyte to be determined or of a substance derived from the analyte, wherein the analyte or the substance derived from the analyte is converted enzymatically in the interference-reducing layer into end products which cannot contribute to the signal formation by the indicator substance.
Claim 2
Dependent Claim
166 words
Analytical element for determining the amount of an analyte in a liquid, the element comprising a sample application zone and a detection zone which are in liquid-transferring contact, the detection zone containing at least one enzyme and an indicator substance, the enzyme catalysing a reaction in which the analyte or a substance derived from the analyte participates and the indicator substance forming a signal when the analyte is present which signal correlates with the amount of analyte, and a liquid-permeable interference-reducing layer in direct contact with the detection zone arranged such that liquid does not reach the detection zone until the liquid has passed through the interference-reducing layer, the interference-reducing layer containing at least one enzyme which participates in a reaction of the analyte to be determined or of a substance derived from the analyte, wherein the analyte or the substance derived from the analyte is converted enzymatically in the interference-reducing layer into end products which cannot contribute to the signal formation by the indicator substance.
Claim 3
Dependent Claim
20 words
Analytical element as claimed in claim 1 , wherein the interference-reducing layer and detection zone are arranged above one another.
Claim 4
Dependent Claim
31 words
Analytical element as claimed in 2 , wherein the interference-reducing layer and the layer of the detection zone containing enzyme and indicator substance are arranged in direct contact with one another.
Claim 5
Dependent Claim
31 words
Analytical element as claimed in 1 , wherein the interference-reducing layer and the layer of the detection zone containing enzyme and indicator substance are arranged in direct contact with one another.
Claim 6
Dependent Claim
25 words
Analytical element as claimed in claim 1 , wherein a layer is located in the sample application zone that retains particles contained in the liquid.
Claim 7
Dependent Claim
25 words
Analytical element as claimed in claim 2 , wherein a layer is located in the sample application zone that retains particles contained in the liquid.
Claim 8
Dependent Claim
25 words
Analytical element as claimed in claim 4 , wherein a layer is located in the sample application zone that retains particles contained in the liquid.
Claim 9
Dependent Claim
17 words
Analytical element as claimed in 1 , wherein the interference-reducing layer is in the sample application zone.
Claim 10
Dependent Claim
17 words
Analytical element as claimed in 2 , wherein the interference-reducing layer is in the sample application zone.
Claim 11
Dependent Claim
17 words
Analytical element as claimed in 4 , wherein the interference-reducing layer is in the sample application zone.
Claim 12
Dependent Claim
19 words
Analytical element as claimed in claim 1 , wherein the end product formed in the interference-reducing layer is colorless.
Claim 13
Dependent Claim
19 words
Analytical element as claimed in claim 2 , wherein the end product formed in the interference-reducing layer is colorless.
Claim 14
Dependent Claim
19 words
Analytical element as claimed in claim 4 , wherein the end product formed in the interference-reducing layer is colorless.
Claim 15
Dependent Claim
19 words
Analytical element as claimed in claim 5 , wherein the end product formed in the interference-reducing layer is colorless.
Claim 16
Dependent Claim
19 words
Analytical element as claimed in claim 8 , wherein the end product formed in the interference-reducing layer is colorless.
Claim 17
Dependent Claim
30 words
Analytical element as claimed in claim 1 , wherein the layer of the detection zone containing enzyme contains the necessary coenzyme if the enzyme of the detection layer is coenzyme-dependent.
Claim 18
Dependent Claim
30 words
Analytical element as claimed in claim 2 , wherein the layer of the detection zone containing enzyme contains the necessary coenzyme if the enzyme of the detection layer is coenzyme-dependent.
Claim 19
Dependent Claim
30 words
Analytical element as claimed in claim 4 , wherein the layer of the detection zone containing enzyme contains the necessary coenzyme if the enzyme of the detection layer is coenzyme-dependent.
Claim 20
Dependent Claim
30 words
Analytical element as claimed in claim 5 , wherein the layer of the detection zone containing enzyme contains the necessary coenzyme if the enzyme of the detection layer is coenzyme-dependent.
Claim 21
Dependent Claim
30 words
Analytical element as claimed in claim 8 , wherein the layer of the detection zone containing enzyme contains the necessary coenzyme if the enzyme of the detection layer is coenzyme-dependent.
Claim 22
Dependent Claim
30 words
Analytical element as claimed in claim 11 , wherein the layer of the detection zone containing enzyme contains the necessary coenzyme if the enzyme of the detection layer is coenzyme-dependent.
Claim 23
Independent Claim
228 words
A method for the determination of an analyte in a liquid, the method comprising the steps of: providing a multilayer analytical element comprising a sample application zone, a detection zone in liquid-transferring contact with the sample application zone, the detection zone containing at least one enzyme and an indicator substance, and a liquid-permeable interference-reducing layer in direct contact with the detection zone arranged such that liquid does not reach the detection zone until the liquid has passed through the interference-reducing layer, the interference-reducing layer containing at least one enzyme which participates in a reaction of the analyte to be determined or of a substance derived from the analyte, applying the liquid to the sample application zone in an undosed manner so that the liquid passes through the interference-reducing layer into the detection zone and fills the detection zone where the enzyme catalyzes a reaction in which the analyte or a substance derived from the analyte participates and forms a signal when the analyte is present which signal correlates with the amount of analyte present in the liquid and excess liquid remains in the interference-reducing layer is exposed to the at least one enzyme which enzymatically converts the analyte or the substance derived from the analyte into end products which cannot contribute to the signal formation by the indicator substance, and determining the amount of analyte in the liquid.
Claim 24
Independent Claim
228 words
A method for the determination of an analyte in a liquid, the method comprising the steps of: providing a multilayer analytical element comprising a sample application zone, a detection zone in liquid-transferring contact with the sample application zone, the detection zone containing at least one enzyme and an indicator substance, and a liquid-permeable interference-reducing layer in direct contact with the detection zone arranged such that liquid does not reach the detection zone until the liquid has passed through the interference-reducing layer, the interference-reducing layer containing at least one enzyme which participates in a reaction of the analyte to be determined or of a substance derived from the analyte, applying the liquid to the sample application zone in an undosed manner so that the liquid passes through the interference-reducing layer into the detection zone and fills the detection zone where the enzyme catalyzes a reaction in which the analyte or a substance derived from the analyte participates and forms a signal when the analyte is present which signal correlates with the amount of analyte present in the liquid and excess liquid remains in the interference-reducing layer is exposed to the at least one enzyme which enzymatically converts the analyte or the substance derived from the analyte into end products which cannot contribute to the signal formation by the indicator substance, and determining the amount of analyte in the liquid.
Claim 25
Dependent Claim
25 words
The method of claim 22 wherein the applying step includes filling the detection zone with liquid so that excess liquid remains in the application zone.
Claim 26
Dependent Claim
31 words
Method as claimed in claim 22 , wherein the liquid to be examined at least partially passes through the interference-reducing layer before the enzyme of the interference-reducing layer begins to act.
Claim 27
Dependent Claim
26 words
Analytical element as claimed in claim 1 , wherein the signal generated by the indicator substance in the presence of the analyte is a color change.
Claim 28
Dependent Claim
20 words
Analytical element as claimed in claim 25 , wherein the interference-reducing layer and detection zone are arranged above one another.
Claim 29
Dependent Claim
32 words
Analytical element as claimed in claim 25 , wherein the interference-reducing layer and the layer of the detection zone containing enzyme and indicator substance are arranged in direct contact with one another.
Claim 30
Dependent Claim
18 words
Analytical element as claimed in claim 25 , wherein the interference-reducing layer is in the sample application zone.
Claim 31
Dependent Claim
19 words
Analytical element as claimed in claim 25 , wherein the end product formed in the interference-reducing layer is colorless.
Claim 32
Dependent Claim
30 words
Analytical element as claimed in claim 25 , wherein the layer of the detection zone containing enzyme contains the necessary coenzyme if the enzyme of the detection layer is coenzyme-dependent.
Claim 33
Dependent Claim
25 words
Analytical element as claimed in claim 25 , wherein a layer is located in the sample application zone that retains particles contained in the liquid.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2006-01-13 | 2006-07-15 | 2007-01-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2010-01-13 | 2010-07-15 | 2011-01-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2014-01-13 | 2014-07-15 | 2015-01-14 | $7700.00 | Window Closed / Lapsed |