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USRE29498E
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Process for the enzymatic determination of glucose with a glucose-oxydazed/peroxidazed enzyme system
Assignee: Istituto Sieroterapico e Vaccinogeno Toscano Sclavo SpA
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Filing: 1976-03-02
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Granted: 1977-12-20
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Expires: 1996-03-02
Abstract
Abstract Process for enzymatic determination of glucose with a glucose-oxidase-peroxidase enzyme system involving the use of a reduced acceptor which is oxidized. As reduced acceptor in the enzymatic reaction catalyzed by peroxidase, a ferrocyanide salt is oxidized to ferricyanide; then a further reducing system is introduced, which system is oxidized by the ferricyanide, with the formation of a colored quinone compound, the reaction between ferricyanide and the reducing system taking place spontaneously.
Inventors
- 👤 Franco Meiattini
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 17:00
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; C12Q2326/00; C12Q2326/50; C12Q2326/90; C12Q2326/96
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
107 words
A process for the enzymatic determination of the amount of glucose present in body fluids which comprises the steps: i. adding to a sample of a body fluid an enzymatic system containing glucose-oxidase and peroxidase, to which has been added sodium or potassium ferrocyanide, ii. incubating the sample and enzyme system at room temperature whereby the ferrocyanide is converted to ferricyanide equivalent to the amount of glucose present in the sample, and iii. reacting the ferricyanide with a reducing system which forms a colored product on oxidation, the color being a measure of the ferricyanide present, said reducing system consisting of a phenolic compound and an aminoantipyrine.
Claim 2
Dependent Claim
107 words
A process for the enzymatic determination of the amount of glucose present in body fluids which comprises the steps: i. adding to a sample of a body fluid an enzymatic system containing glucose-oxidase and peroxidase, to which has been added sodium or potassium ferrocyanide, ii. incubating the sample and enzyme system at room temperature whereby the ferrocyanide is converted to ferricyanide equivalent to the amount of glucose present in the sample, and iii. reacting the ferricyanide with a reducing system which forms a colored product on oxidation, the color being a measure of the ferricyanide present, said reducing system consisting of a phenolic compound and an aminoantipyrine.
Claim 3
Dependent Claim
26 words
A process according to claim 1 wherein the phenolic compound is selected from the group consisting of phenol, salicylamide, barbituric acid, and esters of hydroxybenzoic acid.
Claim 4
Dependent Claim
12 words
A process according to claim 2 wherein the phenolic compound is phenol.
Claim 5
Dependent Claim
11 words
A process according to claim 3 wherein the aminoantipyrine is 4-aminoantipyrine.
Claim 6
Dependent Claim
42 words
A composition for use in the determination of glucose in body fluids by the method of claim 1, which comprises i. an enzymatic system consisting of glucose-oxidase and peroxidase, ii. sodium or potassium ferrocyanide, iii. a phenolic compound, and iv. an aminoantipyrine.
Claim 7
Dependent Claim
19 words
A composition according to claim 5 wherein said composition is buffered at a pH between about 5.5 and 8.
Claim 8
Dependent Claim
43 words
A composition according to claim 5 which is in two parts, the first part comprising i. an enzymatic system consisting of glucose-oxidase and peroxidase, and ii. sodium or potassium ferrocyanide, and the second part comprising iii. a phenolic compound and iv. an aminoantipyrine.
Claim 9
Dependent Claim
41 words
A composition according to claim 5 which is in two parts, the first part comprising i. an enzymatic system consisting of glucose-oxidase and peroxidase, ii. sodium or potassium ferrocyanide, and iii. a phenolic compound, and the second part comprising an aminoantipyrine.
Claim 10
Dependent Claim
23 words
A composition according to claim 8 wherein the part containing the enzyme system is buffered at a pH between about 5.5 to 8.
Claim 11
Dependent Claim
12 words
A composition according to claim 6 wherein the phenolic compound is phenol.
Claim 12
Dependent Claim
11 words
A composition according to claim 10 wherein the aminoantipyrine is 4-aminoantipyrine.
Claim 13
Dependent Claim
12 words
A composition according to claim 9 wherein the phenolic compound is phenol.
Claim 14
Dependent Claim
12 words
A composition according to claim 12 wherein the aminoantipyrine compound is 4-aminoantipyrine.
Claim 15
Dependent Claim
153 words
A composition according to claim 7 wherein the part containing the enzyme system is buffered at a pH between about 5.5 to 8. .Iadd. 15. A process for the enzymatic determination of the amount of glucose in an undeproteinized body fluid which consists in adding to said undeproteinized body fluid an aqueous solution of an enzymatic system consisting of glucose-oxidase and peroxidase, an aminoantipyrine and a phenolic compound which is a member of the group consisting of salicylamide, barbituric acid, para- and meta-hydroxy-benzoate and esters thereof, and thereafter measuring color change photometrically. .Iaddend. .Iadd. 16. A composition for use in the enzymatic determination of the amount of glucose in a body fluid, consisting essentially of a glucose-oxidase and peroxidase enzyme system, a phenolic compound selected from the group consisting of salicylamide, barbituric acid, para- and meta-hydroxybenzoate and esters thereof, and an aminoantipyrine. .Iaddend..Iadd. 17. A dry composition as claimed in claim 16. .Iaddend.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-12-19 | 1981-06-20 | 1981-12-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-12-19 | 1985-06-20 | 1985-12-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-12-19 | 1989-06-20 | 1989-12-20 | $7700.00 | Window Closed / Lapsed |