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US4335205A
Expired / Lapsed
US
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Low protein degradation product basal medium for identification of non-fermentative gram-negative bacilli and other microorganisms
Assignee: Individual
•
Filing: 1979-04-06
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Granted: 1982-06-15
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Expires: 1999-04-06
Abstract
Abstract A single basal growth medium for receiving various substrates for the purpose of rapid identification of any species of non-fermentative Gram-negative bacilli (NFB), wherein the medium is low in organic nitrogen but is supplemented with inorganic nitrogen from an ammonium ion source to enhance NFB growth. The medium also serves to identify members of the family Enterobacteriaceae, cytochrome oxidase positive fermenters, Gram-positive bacilli, Gram-positive cocci, and anaerobes.
Inventors
- 👤 James R. Greenwood
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 20:51
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/045; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/874; Y10S435/875
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
120 words
A single low organic nitrogen basal growth medium for the relatively rapid identification of non-fermentative Gram-negative bacilli (NFB) and other microorganisms including the family Enterobacteriaceae, and cytochrome oxidase positive fermenters, comprising: about 0.5 to about 1.5 gm. of an ammonium ion source, about 0.1 to about 0.3 gm. of a potassium salt, about 0.1 to about 0.3 gm. of a magnesium salt, about 0.2 to about 1.0 gm. of yeast extract, about 0.2 to about 1.0 gm. of an organic nitrogen source, 0.0 to about 60.0 gm. of agar, about 0.002 to about 0.060 gm. of an indicator selected from the group consisting of brom cresol purpose, brom thymol blue, and phenol red, and water sufficient to make 1000 ml.
Claim 2
Dependent Claim
120 words
A single low organic nitrogen basal growth medium for the relatively rapid identification of non-fermentative Gram-negative bacilli (NFB) and other microorganisms including the family Enterobacteriaceae, and cytochrome oxidase positive fermenters, comprising: about 0.5 to about 1.5 gm. of an ammonium ion source, about 0.1 to about 0.3 gm. of a potassium salt, about 0.1 to about 0.3 gm. of a magnesium salt, about 0.2 to about 1.0 gm. of yeast extract, about 0.2 to about 1.0 gm. of an organic nitrogen source, 0.0 to about 60.0 gm. of agar, about 0.002 to about 0.060 gm. of an indicator selected from the group consisting of brom cresol purpose, brom thymol blue, and phenol red, and water sufficient to make 1000 ml.
Claim 3
Dependent Claim
16 words
The invention according to claim 1 in which: said ammonium ion source is an ammonium salt.
Claim 4
Dependent Claim
31 words
The invention according to claim 1 in which: the ammonium ion source is one selected from the group consisting of ammonium chloride, ammonium sulfate, ammonium hypophosphate, ammonium hypophosphite, and ammonium orthophosphate.
Claim 5
Dependent Claim
17 words
The invention according to claim 1 in which: the organic nitrogen source is a protein degradation product.
Claim 6
Dependent Claim
30 words
The invention according to claim 1 in which: the organic nitrogen source is one selected from the group consisting of protein hydrolysate, trypticase, casamino acids, Poly peptone, and pancrearic digest.
Claim 7
Dependent Claim
15 words
The invention according to claim 1 in which: the organic nitrogen source is a peptone.
Claim 8
Dependent Claim
19 words
The invention according to claim 1 in which: said medium has a pH range of about 6.2 to 7.8.
Claim 9
Dependent Claim
55 words
The invention according to claim 1 including: a 2 to 25 gm. substrate alkalinized in said medium, said substrate being one selected from the group consisting of salts of dicarboxylic acids, hydroxyacids, sugar derivatives, ring amino acids, amides, allantoin, asparagine, and gelatin, the pH range being between 7 and 7.8, and 0.1% by weight dextrose.
Claim 10
Dependent Claim
41 words
The invention according to claim 1 including: a 2 to 25 gm. substrate acidified in said medium, said substrate being one selected from the group consisting of polyalcohols, disaccharides, pentoses, heptoses, and hexoses, the pH range being between 6.2 and 7.
Claim 11
Dependent Claim
35 words
The invention according to claim 1 including: a 2 to 25 gm. gelain used as a substrate in said medium to be hydrolyzed, and said hydrolosis being detected by an alkaline shift in said medium.
Claim 12
Dependent Claim
102 words
A kit for the relatively rapid identification of non-fermentative Gram-negative bacilli (NFB) and other microorganisms including the family Enterobacteriaceae, and cytochrome oxidase positive fermenter, comprising: a multiple compartmented tray, each compartment containing a single low organic nitrogen basal growth medium including, about 0.05 to about 0.15 by weight % of an ammonium ion source, about 0.01 to about 0.03 by weight % of a potassium salt, about 0.01 to about 0.03 by weight % of a magnesium salt, about 0.02 to about 0.10 by weight % of yeast extract, about 0.02 to about 0.10 by weight % of an organic nitrogen source,
Claim 13
Dependent Claim
102 words
A kit for the relatively rapid identification of non-fermentative Gram-negative bacilli (NFB) and other microorganisms including the family Enterobacteriaceae, and cytochrome oxidase positive fermenter, comprising: a multiple compartmented tray, each compartment containing a single low organic nitrogen basal growth medium including, about 0.05 to about 0.15 by weight % of an ammonium ion source, about 0.01 to about 0.03 by weight % of a potassium salt, about 0.01 to about 0.03 by weight % of a magnesium salt, about 0.02 to about 0.10 by weight % of yeast extract, about 0.02 to about 0.10 by weight % of an organic nitrogen source,
Claim 14
Dependent Claim
39 words
00 to about 6.0 by weight % of agar, a selected substrate for each compartment capable of being either acidified or alkalinized, and in each compartment the selected indicator for the selected substrate capable of showing a positive reaction.
Claim 15
Dependent Claim
39 words
00 to about 6.0 by weight % of agar, a selected substrate for each compartment capable of being either acidified or alkalinized, and in each compartment the selected indicator for the selected substrate capable of showing a positive reaction.
References Cited (Backward Citations)
US3399115A
Cited
US4048016A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-06-14 | 1985-12-14 | 1986-06-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-06-14 | 1989-12-14 | 1990-06-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-06-14 | 1993-12-14 | 1994-06-15 | $7700.00 | Window Closed / Lapsed |