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US4337242A
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US
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Vaccine stabilizer containing L-glutamic acid and L-arginine
Assignee: Merck and Co Inc
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Filing: 1981-03-10
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Granted: 1982-06-29
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Expires: 2001-03-10
Abstract
Abstract An improved stabilized liquid live viral vaccine contains a live virus, partially hydrolyzed gelatin, a monosaccharide or disaccharide, a cell culture medium, L-glutamic acid, L-arginine and sufficient physiologically acceptable acidic buffer to maintain the pH at from about 6.0 to about 6.5.
Inventors
- 👤 Henry Z. Markus
- 👤 William J. McAleer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 09:01
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K47/00; A61K47/06; A61K47/16; A61K47/18; A61K47/183; A61K47/26; A61K47/30; A61K47/42; A61K9/00; A61K9/0012; A61K9/0019; A61K2039/51; A61K2039/525; C; C12; C12N; C12N2760/00; C12N2760/00011; C12N2760/18011; C12N2760/18411; C12N2760/18434; Y; Y02; Y02A; Y02A50/00; Y02A50/30
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
102 words
A liquid vaccine comprising an inactivated or attenuated virus and a stabilizer consisting essentially of on a parts by weight basis from about 1.5 to about 2.1 parts partially hydrolyzed gelatin having a molecular weight of about 3,000, from about 7.0 to about 13.0 parts of a monosaccharide or disaccharide from about 0.4 to about 0.6 parts of an in vitro cell culture medium, from about 0.35 to about 0.7 part L-glutamic acid, from about 0.75 to about 1.3 parts L-arginine, and an amount of a physiologically acceptable acidic buffer effective to maintain the pH to from about 6.0 to about 6.5.
Claim 2
Dependent Claim
102 words
A liquid vaccine comprising an inactivated or attenuated virus and a stabilizer consisting essentially of on a parts by weight basis from about 1.5 to about 2.1 parts partially hydrolyzed gelatin having a molecular weight of about 3,000, from about 7.0 to about 13.0 parts of a monosaccharide or disaccharide from about 0.4 to about 0.6 parts of an in vitro cell culture medium, from about 0.35 to about 0.7 part L-glutamic acid, from about 0.75 to about 1.3 parts L-arginine, and an amount of a physiologically acceptable acidic buffer effective to maintain the pH to from about 6.0 to about 6.5.
Claim 3
Dependent Claim
11 words
A vaccine according to claim 1 wherein the disaccharide is sucrose.
Claim 4
Dependent Claim
12 words
A vaccine according to claim 1 wherein the buffer is phosphate buffer.
Claim 5
Dependent Claim
27 words
A vaccine according to claim 1 wherein the virus is measles, mumps, rubella, varicella, polio, hepatitis, herpes simplex type 1, herpes simplex type 2, or combinations thereof.
Claim 6
Dependent Claim
80 words
A vaccine according to claim 1 wherein the stabilizer consists essentially of on a parts by weight basis of about 1.8 parts partially hydrolyzed gelatin having a molecular weight of about 3,000, about 10.0 parts of a monosaccharide or disaccharide, about 0.5 part of an in vitro cell culture medium, about 0.5 part L-glutamic acid, about 1.0 part L-arginine, and an amount of a physiologically acceptable acidic buffer effective to adjust the pH to from about 6.0 to about 6.5.
Claim 7
Dependent Claim
11 words
A vaccine according to claim 5 wherein the disaccharide is sucrose.
References Cited (Backward Citations)
JPS4810523B1
Cited
FR2076787A5
Cited
US3783098A
Cited
DE2405675A1
Cited
US4147772A
Cited
US4273762A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-06-28 | 1985-12-28 | 1986-06-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-06-28 | 1989-12-28 | 1990-06-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-06-28 | 1993-12-28 | 1994-06-29 | $7700.00 | Window Closed / Lapsed |