Patent US4434093A successfully refreshed from Google Patents.
US4434093A
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Methods for preparation of HBs Ag free gamma globulins
Assignee: Ortho Clinical Diagnostics Inc
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Filing: 1982-07-26
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Granted: 1984-02-28
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Expires: 2002-07-26
Abstract
Abstract Methods for producing from human serum, human gamma globulin essentially free of HB s Ag and products resulting therefrom. Specifically provided are ion exchange resin/buffer systems capable of effectively removing HB s Ag thought to be closely correlated with viral hepatitis type B infectivity. Additional steps can include ultrafiltration to increase reduction of any virus not removed in the column passage as well as the addition of anti-HB g to substantially eliminate all infectivity.
Inventors
- 👤 Raymond P. Zolton
- 👤 Paul M. Kaplan
- 👤 John V. Padvelskis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 22:20
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/06; C07K16/065; B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/36; B01D15/361; B01D61/00; B01D61/14; B01D61/145; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
135 words
A method for removing substantially all HB s Ag from a gamma globulin containing body fluid comprising the steps of: (a) providing the gamma globulin containing body fluid desired to be purified; (b) applying the body fluid to column means containing an effective amount of resin selected from the group consisting of DEAE-Sephadex and QAE-Sephadex; (c) eluting the body fluid from the column with a buffer selected from the group consisting of approximately 0.02 M phosphate buffer if the resin selected is QAE-Sephadex and approximately 0.04 M Tris buffer if the resin selected is DEAE-Sephadex or QAE-Sephadex, each buffer adjusted to a pH of approximately 7.5; (d) monitoring the column effluent for the presence of protein; (e) collecting, responsive to monitoring, the protein containing effluent whereby substantially HB s Ag free gamma globulin is obtained.
Claim 2
Independent Claim
135 words
A method for removing substantially all HB s Ag from a gamma globulin containing body fluid comprising the steps of: (a) providing the gamma globulin containing body fluid desired to be purified; (b) applying the body fluid to column means containing an effective amount of resin selected from the group consisting of DEAE-Sephadex and QAE-Sephadex; (c) eluting the body fluid from the column with a buffer selected from the group consisting of approximately 0.02 M phosphate buffer if the resin selected is QAE-Sephadex and approximately 0.04 M Tris buffer if the resin selected is DEAE-Sephadex or QAE-Sephadex, each buffer adjusted to a pH of approximately 7.5; (d) monitoring the column effluent for the presence of protein; (e) collecting, responsive to monitoring, the protein containing effluent whereby substantially HB s Ag free gamma globulin is obtained.
Claim 3
Dependent Claim
25 words
The method as provided in claim 1 wherein the effective amount of resin is an amount at least equal to 160 mg/ml of body fluid.
Claim 4
Dependent Claim
20 words
The method as provided in claim 2 wherein the resin is QAE-Sephadex and the buffer is 0.02 M phosphate buffer.
Claim 5
Dependent Claim
21 words
The method as provided in claim 2 wherein the resin is QAE-Sephadex and the buffer is approximately 0.04 M Tris buffer.
Claim 6
Dependent Claim
21 words
The method as provided in claim 2 wherein the resin is DEAE-Sephadex and the buffer is approximately 0.04 M Tris buffer.
Claim 7
Independent Claim
105 words
A method for removing substantially all HB s Ag from a gamma globulin containing body fluid comprising the steps of: (a) providing the gamma globulin contained body fluid desired to be purified; (b) applying the body fluid to a column means containing QAE-Sephadex in an amount at least equal to 160 mg/ml of body fluid; (c) eluting the body fluid from the column with approximately 0.05 M Imidazole buffer adjusted to a pH of approximately 7.5; (d) monitoring the column effluent for the presence of protein; (e) collecting, responsive to monitoring, the protein containing effluent whereby substantially HB s Ag free gamma globulin is obtained.
Claim 8
Independent Claim
105 words
A method for removing substantially all HB s Ag from a gamma globulin containing body fluid comprising the steps of: (a) providing the gamma globulin contained body fluid desired to be purified; (b) applying the body fluid to a column means containing QAE-Sephadex in an amount at least equal to 160 mg/ml of body fluid; (c) eluting the body fluid from the column with approximately 0.05 M Imidazole buffer adjusted to a pH of approximately 7.5; (d) monitoring the column effluent for the presence of protein; (e) collecting, responsive to monitoring, the protein containing effluent whereby substantially HB s Ag free gamma globulin is obtained.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-02-27 | 1987-08-29 | 1988-02-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-02-27 | 1991-08-29 | 1992-02-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-02-27 | 1995-08-29 | 1996-02-28 | $7700.00 | Window Closed / Lapsed |