US4694074A
Expired / Lapsed
US
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Process for the purification of HBsAg
Assignee: Tanabe Pharma Corp
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Filing: 1985-10-28
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Granted: 1987-09-15
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Expires: 2005-10-28
Abstract
Abstract A process for the purification of HBsAg is disclosed, which comprises adsorbing specifically on a carrier, in the presence of an inorganic salt in an amount of 5 to 25 W/V %, an HBsAg obtained by gene engineering.
Inventors
- 👤 Yahiro Uemura
- 👤 Takao Ohmura
- 👤 Akimasa Ohmizu
- 👤 Akinori Sumi
- 👤 Wataru Ohtani
- 👤 Yoshitaka Sakanishi
- 👤 Hiroshi Morise
- 👤 Hirofumi Arimura
- 👤 Tadakazu Suyama
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 23:19
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/04; C07K14/00; C07K14/005; A; A61; A61K; A61K39/00; A61K39/12; A61K39/29; A61P; A61P1/00; A61P1/16; C12; C12N; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122; Y; Y10; Y10S; Y10S530/00; Y10S530/806; Y10S530/808
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
129 words
A process for the purification of HBsAg obtained by DNA recombination, which comprises adding an inorganic salt to a crude HBsAg solution, said HBsAg having been obtained by DNA recombination, in a concentration so that the resultant HBsAg solution has an inorganic salt concentration of five to twenty-five W/V percent, bringing the resultant solution into contact with a fixing carrier having hydrophobic groups selected from the group consisting of a phenylalanine residue, an alkyl group containing two to eight carbon atoms and a phenyl group, said carrier having been equilibrated with an inorganic salt solution of the same concentration as the resultant HBsAg solution, to adsorb thereon HBsAg with a solution of the inorganic salt to wash away contaminating free proteins and eluting the HBsAg with a buffer solution.
Claim 2
Dependent Claim
129 words
A process for the purification of HBsAg obtained by DNA recombination, which comprises adding an inorganic salt to a crude HBsAg solution, said HBsAg having been obtained by DNA recombination, in a concentration so that the resultant HBsAg solution has an inorganic salt concentration of five to twenty-five W/V percent, bringing the resultant solution into contact with a fixing carrier having hydrophobic groups selected from the group consisting of a phenylalanine residue, an alkyl group containing two to eight carbon atoms and a phenyl group, said carrier having been equilibrated with an inorganic salt solution of the same concentration as the resultant HBsAg solution, to adsorb thereon HBsAg with a solution of the inorganic salt to wash away contaminating free proteins and eluting the HBsAg with a buffer solution.
Claim 3
Dependent Claim
34 words
The process as claimed in claim 1, wherein the hydrophobic group is selected from the group consisting of a phenylalanine residue, an alkyl group containing 2 to 4 carbon atoms and a phenyl group.
Claim 4
Dependent Claim
43 words
The process as claimed in claim 1, wherein the inorganic salt is consisting of at least one anion selected from the group consisting of sulfate, phosphate and chloride, and at least one cation selected from the group consisting of ammonium, sodium and potassium.
Claim 5
Dependent Claim
22 words
The process as claimed in claim 3, wherein the inorganic salt is ammonium sulfate, sodium sulfate, sodium chloride, or a phosphate compound.
Claim 6
Dependent Claim
28 words
The process as claimed in claim 1, wherein the fixing carrier is selected from the group consisting of an amino acid polymer, cellulose, agarose, dextran and polyacrylic amide.
Claim 7
Dependent Claim
34 words
The process as claimed in claim 1, wherein the buffer solution for elution contains 5 to 20 V/V% of ethanol and has a pH of 6 to 8 or is a phosphate buffer solution.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1990-09-14 | 1991-03-16 | 1991-09-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-09-14 | 1995-03-16 | 1995-09-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-09-14 | 1999-03-16 | 1999-09-15 | $7700.00 | Window Closed / Lapsed |