US4746647A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Purifying protein or peptide recombinant DNA products by electroseparation
Assignee: Individual
•
Filing: 1985-05-24
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Granted: 1988-05-24
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Expires: 2005-05-24
Abstract
Abstract A method of purifying a contaminated product (protein/peptide) produced by rDNA technique, from hydrophobic contaminants which are charged and originate from the microbial cloning host employed, and the product thus purified are disclosed. The contaminated product is subjected to electroseparation, such as electrodialysis, to remove the contaminants. A charge-providing pretreatment is resorted to when the contaminants themselves are not charged enough to enable them to be directly separated upon electroseparation. In addition, a method of checking the purity of a product (protein/peptide) produced by rDNA technique is disclosed. Moreover, a method of checking the efficiency of the purification process used for purifying a product is disclosed.
Inventors
- 👤 Stefan Svenson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 23:19
CPC Classifications
C; C12; C12N; C12N15/00; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; B; B01; B01D; B01D61/00; B01D61/42; B01D61/44; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/62; C12P; C12P21/00; C12P21/02; B01D2311/00; B01D2311/04
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
80 words
A method of purifying a protein/peptide product produced by rDNA techniques using a microbial cloning host, to remove charged hydrophobic contaminants which originate from the microbial cloning host and which form epitopes and fusion epitopes with the product, which comprises subjecting the product containing the epitopes and fusion epitopes to electroseparation under conditions which separate the hydrophobic contaminant from the product to yield a purified product which will not give rise to immune reactions upon administration to mammals, including man.
Claim 2
Independent Claim
80 words
A method of purifying a protein/peptide product produced by rDNA techniques using a microbial cloning host, to remove charged hydrophobic contaminants which originate from the microbial cloning host and which form epitopes and fusion epitopes with the product, which comprises subjecting the product containing the epitopes and fusion epitopes to electroseparation under conditions which separate the hydrophobic contaminant from the product to yield a purified product which will not give rise to immune reactions upon administration to mammals, including man.
Claim 3
Dependent Claim
25 words
A method as claimed in claim 1, wherein the hydrophobic contaminants are given at least a part of their charge by a charge providing pretreatment.
Claim 4
Dependent Claim
29 words
A method as claimed in claim 2, wherein the pretreatment comprises a mild alkaline treatment, dissociation by means of dissociating agent, treatment with kaotropic ions, or with chelate-forming substances.
Claim 5
Dependent Claim
14 words
A method as claimed in claim 1, wherein the electroseparation is conducted by electrodialysis.
Claim 6
Dependent Claim
13 words
A method as claimed in claim 1, wherein the contaminated product is insulin.
Claim 7
Dependent Claim
18 words
A method as claimed in claim 1, wherein the hydrophobic contaminants consist of lipopolysaccarides originating from Escherichia coli.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-05-24 | 1991-11-23 | 1992-05-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-05-24 | 1995-11-23 | 1996-05-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-05-24 | 1999-11-23 | 2000-05-24 | $7700.00 | Window Closed / Lapsed |