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Patent US4789702A successfully refreshed from Google Patents.
US4789702A
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Feline leukemia virus vaccine
Assignee: Novartis Vaccines and Diagnostics Inc
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Filing: 1986-06-12
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Granted: 1988-12-06
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Expires: 2006-06-12
Abstract
Abstract Polypeptides that are synthesized or expressed directly in microorganisms, include an amino acid sequence that is homologous to at least a portion of gp70 envelope protein of FELV, and are immunogens that elicit or prime a humoral response in cats and immunize cats against FeLV infection. The microbially produced polypeptides are exemplified by a group of E. coli-expressed fusion proteins that comprise a portion of the trp leader peptide and a portion of the trp E protein fused to various portions of a subgroup B gp70 envelope protein. The synthesized polypeptides are exemplified by polypeptides that are homologous to segments of the hydrophilic domains of the gp70 protein.
Inventors
- 👤 Jack H. Nunberg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 22:06
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/62; A; A61; A61K; A61K39/00; A61K39/12; A61K39/21; C07; C07K; C07K14/00; C07K14/005; A61K2039/545; A61K2039/55; A61K2039/552; A61K2039/555; A61K2039/55511; A61K2039/55566; A61K2039/57; A61K2039/60; A61K2039/6031; A61K2039/6081; A61K38/00; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/40; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/13011; C12N2740/13022; C12N2740/13034; Y; Y10; Y10S; Y10S530/00; Y10S530/806; Y10S530/81; Y10S530/812; Y10S530/82; Y10S930/00; Y10S930/01; Y10S930/30
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
71 words
A microbially produced polypeptide comprising an amino acid sequence that is homologous to at least a portion of the amino acid sequence of gp85 envelope protein of feline leukemia virus subgroup B that occurs in the 210-250 region and the 415-450 region of the FeLV gp85 protein, and is an immunogen that primes or elicits a humoral response in cats and is useful for immunizing cats against feline leukemia virus infection.
Claim 2
Dependent Claim
71 words
A microbially produced polypeptide comprising an amino acid sequence that is homologous to at least a portion of the amino acid sequence of gp85 envelope protein of feline leukemia virus subgroup B that occurs in the 210-250 region and the 415-450 region of the FeLV gp85 protein, and is an immunogen that primes or elicits a humoral response in cats and is useful for immunizing cats against feline leukemia virus infection.
Claim 3
Dependent Claim
20 words
The polypeptide of claim 1 wherein the gp70 envelope protein is from feline leukemia virus strain GA or strain ST.
Claim 4
Dependent Claim
41 words
The polypeptide of claim 1 wherein the amino acid sequence is homologous to at least a substantial portion of at least one of the hydrophilic domains of the gp85 protein that occurs in the 210-250 region and in the 415-450 region.
Claim 5
Dependent Claim
75 words
The polypeptide of claim 1 wherein the portion of the amino acid sequence of gp85 envelope protein is (a) met gly pro asn leu val pro asp gln lys pro pro ser; (b) asp gln lys pro pro ser arg gln ser gln ile glu; (c) pro glu tyr val tyr thr his phe asp lys thr val arg leu; or (d) asp lys thr val arg leu arg arg glu pro ile ser leu.
Claim 6
Dependent Claim
15 words
The polypeptide of claim 4 wherein the amino acid sequence is repeated in the polypeptide.
Claim 7
Dependent Claim
78 words
An E. coli produced fusion protein comprising: (a) a trpLE', fusion peptide sequence and (b) an amino acid seqeunce that is homologous to at least a portion of the amino acid sequence of gp85 envelope protein of feline leukemia virus subgroup B that occurs in the 210-250 region and the 415-450 region of the FeLV gp85 protein, said fusion protein being an immunogen that primes or elicits a humoral response in cats and is useful for immunizing cats.
Claim 8
Dependent Claim
78 words
An E. coli produced fusion protein comprising: (a) a trpLE', fusion peptide sequence and (b) an amino acid seqeunce that is homologous to at least a portion of the amino acid sequence of gp85 envelope protein of feline leukemia virus subgroup B that occurs in the 210-250 region and the 415-450 region of the FeLV gp85 protein, said fusion protein being an immunogen that primes or elicits a humoral response in cats and is useful for immunizing cats.
Claim 9
Dependent Claim
21 words
The fusion protein of claim 6 wherein the gp85 envelope protein is from feline leukemia virus strain GA or strain ST.
Claim 10
Dependent Claim
40 words
The fusion protein of claim 6 wherein the amino acid sequence is homologous to a substantial portion of at least one of the hydrophilic domains of the gp85 protein that occurs in the 210-250 region and in the 415-450 region.
Claim 11
Dependent Claim
76 words
The fusion protein of claim 6 wherein the portion of the amino acid sequence of gp85 envelope protein is (a) met gly pro asn leu val leu pro asp gln lys pro pro ser; (b) asp gln lys pro ser arg gln ser gln ile glu; (c) pro glu tyr val tyr thr his phe asp lys thr val arg leu; or (d) asp lys thr val arg leu arg arg glu pro ile ser leu.
Claim 12
Dependent Claim
21 words
The fusion protein of claim 6 wherein the protein is the product of expression of the plasmid ptGAΔAvaI, ptGAΔApaI, or pt9:3-19.
Claim 13
Dependent Claim
56 words
A conjugate of: (a) a polypeptide having an amino acid sequence that is homologous to at least a substantial portion of at least one of the hydrophilic domains of the gp85 envelope protein of feline leukemia virus subgroup B that occurs in the 210-250 region and in the 415-450 region coupled to (b) a carrier protein.
Claim 14
Dependent Claim
56 words
A conjugate of: (a) a polypeptide having an amino acid sequence that is homologous to at least a substantial portion of at least one of the hydrophilic domains of the gp85 envelope protein of feline leukemia virus subgroup B that occurs in the 210-250 region and in the 415-450 region coupled to (b) a carrier protein.
Claim 15
Dependent Claim
92 words
The conjugate of claim 11 wherein the polypeptide comprises the amino acid sequence (a) H - cys asp lys thr val arg leu arg arg glu pro ile ser leu - OH; (b) H - cys pro glu tyr val tyr thr his phe asp lys thr val arg leu - OH; (c) H - cys met gly pro asn leu val leu pro asp gln lys pro pro ser - OH; or (d) H - asp gln lys pro pro ser arg glu ser gln ile glu cys ser - OH.
Claim 16
Dependent Claim
84 words
A polypeptide of the formula (a) H - cys asp lys thr val arg leu arg arg glu pro ile ser leu - OH; (b) H - cys pro glu tyr val tyr thr his phe asp lys thr val arg leu - OH; (c) H - cys met gly pro asn leu val leu pro asp gln lys pro pro ser - OH; or (d) H - asp gln lys pro pro ser arg glu ser gln ile glu cys ser - OH.
Claim 17
Dependent Claim
84 words
A polypeptide of the formula (a) H - cys asp lys thr val arg leu arg arg glu pro ile ser leu - OH; (b) H - cys pro glu tyr val tyr thr his phe asp lys thr val arg leu - OH; (c) H - cys met gly pro asn leu val leu pro asp gln lys pro pro ser - OH; or (d) H - asp gln lys pro pro ser arg glu ser gln ile glu cys ser - OH.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-12-06 | 1992-06-06 | 1992-12-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-12-06 | 1996-06-06 | 1996-12-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-12-06 | 2000-06-06 | 2000-12-06 | $7700.00 | Window Closed / Lapsed |