Patent US4861867A successfully refreshed from Google Patents.
US4861867A
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Purified hemoglobin solutions and method for making same
Assignee: Baxter International Inc
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Filing: 1989-01-23
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Granted: 1989-08-29
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Expires: 2009-01-23
Abstract
Abstract Hemoglobin solutions are purified by heating in the presence of a reducing agent or other deoxygenating conditions such that nonhemoglobin proteins are selectively precipitated and/or virus is inactivated while minimizing hemoglobin loss.
Inventors
- 👤 Timothy N. Estep
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 09:48
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/795; C07K14/805; A; A61; A61L; A61L2/00; A61L2/02; A61L2/04; A61L2103/00; A61L2103/05
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
51 words
The method of precipitating nonhemoglobin proteins from a hemoglobin solution without substantially precipitating or deactivating said hemoglobin which comprises heating substantially cell-free hemoglobin solution at a temperature of 45 degrees to 85 degrees C., while maintaining said hemoglobin in substantially its deoxyhemoglobin form, whereby heat precipitate nonhemoglobin proteins present are precipitated.
Claim 2
Independent Claim
51 words
The method of precipitating nonhemoglobin proteins from a hemoglobin solution without substantially precipitating or deactivating said hemoglobin which comprises heating substantially cell-free hemoglobin solution at a temperature of 45 degrees to 85 degrees C., while maintaining said hemoglobin in substantially its deoxyhemoglobin form, whereby heat precipitate nonhemoglobin proteins present are precipitated.
Claim 3
Dependent Claim
16 words
The method of claim 1 in which said temperature is 60 degrees to 75 degrees C.
Claim 4
Dependent Claim
41 words
The method of claim 2 in which said hemoglobin is maintained in substantially its deoxyhemoglobin form by the presence of a chemical reducing agent under conditions such as the nonhemoglobin protein selectively precipitates but the hemoglobin is not substantially biologically inactivated.
Claim 5
Dependent Claim
19 words
The method of claim 3 in which said reducing agent has a reducing potential which is greater than ascorbate.
Claim 6
Dependent Claim
18 words
The method of claim 1 in which said reducing agent is a reduced dye, sulfoxy, or sulfhydryl compound.
Claim 7
Dependent Claim
17 words
the method of claim 5 wherein the agent is dithionite, bisulphite, metabisulphite, sulphite, reduced glutathione or dithiothreitol.
Claim 8
Dependent Claim
15 words
The method of claim 1 in which said time of heating is about 1-15 hours.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-08-28 | 1993-02-27 | 1993-08-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-08-28 | 1997-02-27 | 1997-08-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-08-28 | 2001-02-27 | 2001-08-29 | $7700.00 | Window Closed / Lapsed |