Ascorbic acid decomposing method

Claim 1

The method for quantitatively measuring a selected component in a sample of body fluid wherein an adverse influence of a reducing substance is prevented, said method consisting essentially of: providing a sample of a body fluid containing a reducing substance, introducing into said sample, copper ions, peroxidase, and at least one compound selected from the group consisting of: 4-aminoantipyrine, 3-methyl-2-benzothiazolinonehydrazone, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid, leucomalachite green, leucocrystal violet, bis(p-diethylaminophenyl)-2-sulfophenylmethane, bis(p-diethylamino-phenyl)-4-sulfopropoxyphenyl methane sodium salt, bis(p-diethylaminophenyl)-3,4-disulfopropoxyphenyl methane disodium salt, phenol compounds, aniline compounds, naphthol compounds, and mixtures thereof; and enzymatically producing hydrogen peroxide in said sample by the action of an enzyme action upon a substrate; and quantifying the thus produced hydrogen peroxide.

Claim 2

The method for quantitatively measuring a selected component in a sample of body fluid wherein an adverse influence of a reducing substance is prevented, said method consisting essentially of: providing a sample of a body fluid containing a reducing substance, introducing into said sample, copper ions, peroxidase, and at least one compound selected from the group consisting of: 4-aminoantipyrine, 3-methyl-2-benzothiazolinonehydrazone, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid, leucomalachite green, leucocrystal violet, bis(p-diethylaminophenyl)-2-sulfophenylmethane, bis(p-diethylamino-phenyl)-4-sulfopropoxyphenyl methane sodium salt, bis(p-diethylaminophenyl)-3,4-disulfopropoxyphenyl methane disodium salt, phenol compounds, aniline compounds, naphthol compounds, and mixtures thereof; and enzymatically producing hydrogen peroxide in said sample by the action of an enzyme action upon a substrate; and quantifying the thus produced hydrogen peroxide.

Claim 5

A method for quantitatively measuring the amount of a selected component in a sample of body fluid containing ascorbic acid, removing the influence of ascorbic acid, said method consisting essentially of the following steps: decomposing the ascorbic acid contained in a sample into products of water and dehydroascorbic acid by adding, concurrently, thereto copper ion, peroxidase and at least one compound selected from the group consisting of: 4-aminoantipyrine, 3-methyl-2-benzothiazolinonehydrazone, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), leucomalachite green, leucocrystal violet, bis(p-diethylaminophenyl)-2-sulfophenyl methane, bis(p-diethylamino-phenyl)-4-sulfopropoxyphenyl methane sodium salt, bis(p-diethyl-aminophenyl)-3,4-disulfopropoxyphenyl methane disodium salt, phenol compounds, aniline compounds, naphthol compounds, and mixtures thereof; and quantifying the amount of said selected component in said sample following an oxidation-reduction reaction of said component.

Claim 6

A method for quantitatively measuring the amount of a selected component in a sample of body fluid containing ascorbic acid, removing the influence of ascorbic acid, said method consisting essentially of the following steps: decomposing the ascorbic acid contained in a sample into products of water and dehydroascorbic acid by adding, concurrently, thereto copper ion, peroxidase and at least one compound selected from the group consisting of: 4-aminoantipyrine, 3-methyl-2-benzothiazolinonehydrazone, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), leucomalachite green, leucocrystal violet, bis(p-diethylaminophenyl)-2-sulfophenyl methane, bis(p-diethylamino-phenyl)-4-sulfopropoxyphenyl methane sodium salt, bis(p-diethyl-aminophenyl)-3,4-disulfopropoxyphenyl methane disodium salt, phenol compounds, aniline compounds, naphthol compounds, and mixtures thereof; and quantifying the amount of said selected component in said sample following an oxidation-reduction reaction of said component.

Claim 7

A method for quantitatively measuring the amount of a selected component in a sample of body fluid containing ascorbic acid, removing the influence of ascorbic acid, said method comprising the following steps: decomposing the ascorbic acid contained in a sample into products of water and dehydroascorbic acid by adding, concurrently, thereto copper ion, peroxidase and at least one compound selected from the group consisting of: 4-aminoantipyrine, 3-methyl-2-benzothiazolinonehydrazone, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), leucomalachite green, leucocrystal violet, bis(p-diethylaminophenyl)-2-sulfophenyl methane, bis(p-diethylamino-phenyl)-4-sulfopropoxyphenyl methane sodium salt, bis(p-diethyl-aminophenyl)-3,4-disulfopropoxyphenyl methane disodium salt, phenol compounds, aniline compounds, naphthol compounds, and mixtures thereof; and quantifying the amount of said selected component in said sample following an oxidation-reduction reaction of said component.

Claim 8

A method for quantitatively measuring the amount of a selected component in a sample of body fluid containing ascorbic acid, removing the influence of ascorbic acid, said method comprising the following steps: decomposing the ascorbic acid contained in a sample into products of water and dehydroascorbic acid by adding, concurrently, thereto copper ion, peroxidase and at least one compound selected from the group consisting of: 4-aminoantipyrine, 3-methyl-2-benzothiazolinonehydrazone, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), leucomalachite green, leucocrystal violet, bis(p-diethylaminophenyl)-2-sulfophenyl methane, bis(p-diethylamino-phenyl)-4-sulfopropoxyphenyl methane sodium salt, bis(p-diethyl-aminophenyl)-3,4-disulfopropoxyphenyl methane disodium salt, phenol compounds, aniline compounds, naphthol compounds, and mixtures thereof; and quantifying the amount of said selected component in said sample following an oxidation-reduction reaction of said component.