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US5069769A
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Electrophoresis pattern reading system of fluorescence type
Assignee: Hitachi Software Engineering Co Ltd
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Filing: 1990-06-06
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Granted: 1991-12-03
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Expires: 2010-06-06
Abstract
Abstract An electrophoresis pattern reading system of fluorescent type is comprised of a detachable migration unit comprising a gel functioning as a base for electrophoresis and a gel-supporting body for supporting the gel; an electrophoresis unit, to which the migration unit is mounted, for performing electrophoresis by applying migrating voltage to the gel to which a sample labeled with a fluorescent substance is added; and a reading unit including an instrumentation subunit for reading an electrophoresis pattern, to which the migration unit is mounted after electrophoresis and which receives fluorescence emitted from the fluorescent substance of the sample on the gel upon application of light to the gel. For example, a plurality of the migration units and the electrophoresis units are provided, and each of the plural migration units are mounted to one reading unit in order after electrophoresis has been performed with the respective electrophoresis units for a long period of time, thereby reading the electrophoresis pattern in a short period of time.
Inventors
- 👤 Hitoshi Fujimiya
- 👤 Shigeo Nakajima
- 👤 Hisanori Nasu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 09:08
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44717; G01N27/44721
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
160 words
An electrophoresis pattern reading system of fluorescence-detection type, useful for analyzing a gel-based sample, the sample being labeled with a fluorescent substance that fluoresces upon application of light thereto, comprising: a detachable migration unit comprising a gel functioning as a base for a sample to be analyzed by electrophoresis and a gel-supporting body for supporting the gel; an electrophoresis unit, to which the migration unit is detachably mounted, for performing electrophoresis by applying migrating voltage to the gel to which the sample labeled with a fluorescent substance is added; and a reading unit physically separate from the electrophoresis unit for reading an electrophoresis pattern, the reading unit including means for detachably mounting the migration unit detached from and apart from the electrophoresis unit after electrophoresis, and said reading unit having means for passing light to the detachably mounted migration unit and for receiving fluorescence emitted from the fluorescent substance of the sample on the gel upon application of the light.
Claim 2
Dependent Claim
160 words
An electrophoresis pattern reading system of fluorescence-detection type, useful for analyzing a gel-based sample, the sample being labeled with a fluorescent substance that fluoresces upon application of light thereto, comprising: a detachable migration unit comprising a gel functioning as a base for a sample to be analyzed by electrophoresis and a gel-supporting body for supporting the gel; an electrophoresis unit, to which the migration unit is detachably mounted, for performing electrophoresis by applying migrating voltage to the gel to which the sample labeled with a fluorescent substance is added; and a reading unit physically separate from the electrophoresis unit for reading an electrophoresis pattern, the reading unit including means for detachably mounting the migration unit detached from and apart from the electrophoresis unit after electrophoresis, and said reading unit having means for passing light to the detachably mounted migration unit and for receiving fluorescence emitted from the fluorescent substance of the sample on the gel upon application of the light.
Claim 3
Dependent Claim
25 words
An electrophoresis pattern reading system of fluorescence-detection type as claimed in claim 1, wherein a plurality of electrophoresis units is provided for each reading unit.
Claim 4
Dependent Claim
74 words
An electrophoresis pattern reading system of fluorescence-detection type as claimed in claim 1, said means for passing light comprising a spot light source for generating light for exciting fluorescence of the fluorescent substance, a scanning means for scanning light from the spot light source in a direction substantially parallel to the direction of electrophoresis by radiation upon the gel, and a reading section having a light-receiving subsection for receiving fluorescence from the fluorescent substance.
Claim 5
Dependent Claim
39 words
An electrophoresis pattern reading system of fluorescence-detection type as claimed in claim 3, wherein the light-receiving subsection comprises a one-dimensional image sensor, wherein the direction in which the light is received is substantially parallel to the direction of electrophoresis.
Claim 6
Dependent Claim
62 words
An electrophoresis pattern reading system of fluorescence-detection type as claimed in claim 3, wherein said means for passing light and said scanning means produces the scanning light of a width in the direction perpendicular to the direction of electrophoresis that is extended on the basis of the reading width of the irradiated area in the direction parallel to the direction of electrophoresis.
Claim 7
Dependent Claim
27 words
An electrophoresis pattern reading system as claimed in claim 3, wherein the scanning means includes a concave lens for modulating the light from the spot light source.
Claim 8
Dependent Claim
41 words
An electrophoresis pattern reading system as claimed in claim 6, wherein the concave lens is arranged to modulate the light from the spot light source to enlarge reading size with respect to a direction substantially perpendicular to the direction of electrophoresis.
Claim 9
Dependent Claim
27 words
An electrophoresis pattern reading system as claimed in claim 3, wherein the scanning means includes a convex lens for modulating the light from the spot light source.
Claim 10
Dependent Claim
23 words
An electrophoresis pattern reading system as claimed in claim 8, wherein the convex lens is disclosed substantially parallel to the direction of electrophoresis.
Claim 11
Dependent Claim
17 words
An electrophoresis pattern reading system as claimed in claim 1, wherein the sample comprises a nucleic acid.
Claim 12
Dependent Claim
74 words
An electrophoresis pattern reading system of fluorescence-detection type as claimed in claim 2, said means for passing light comprising a spot light source for generating light for exciting fluorescence of the fluorescent substance, a scanning means for scanning light from the spot light source in a direction substantially parallel to the direction of electrophoresis by radiation upon the gel, and a reading section having a light-receiving subsection for receiving fluorescence from the fluorescent substance.
Claim 13
Dependent Claim
39 words
An electrophoresis pattern reading system of fluorescence-detection type as claimed in claim 11, wherein the light-receiving subsection comprises a one-dimensional image sensor, wherein the direction in which the light is received is substantially parallel to the direction of electrophoresis.
Claim 14
Dependent Claim
62 words
An electrophoresis pattern reading system of fluorescence-detection type as claimed in claim 11, wherein said means for passing light and said scanning means produces the scanning light of a width in the direction perpendicular to the direction of electrophoresis that is extended on the basis of the reading width of the irradiated area in the direction parallel to the direction of electrophoresis.
Claim 15
Dependent Claim
27 words
An electrophoresis pattern reading system as claimed in claim 11, wherein the scanning means includes a concave lens for modulating the light from the spot light source.
Claim 16
Dependent Claim
41 words
An electrophoresis pattern reading system as claimed in claim 14, wherein the concave lens is arranged to modulate the light from the spot light source to enlarge reading size with respect to a direction substantially perpendicular to the direction of electrophoresis.
Claim 17
Dependent Claim
27 words
An electrophoresis pattern reading system as claimed in claim 11, wherein the scanning means includes a convex lens for modulating the light from the spot light source.
Claim 18
Dependent Claim
23 words
An electrophoresis pattern reading system as claimed in claim 16, wherein the convex lens is disclosed substantially parallel to the direction of electrophoresis.
Claim 19
Dependent Claim
17 words
An electrophoresis pattern reading system as claimed in claim 2, wherein the sample comprises a nucleic acid.
Claim 20
Independent Claim
96 words
A method for reading an electrophoresis pattern of fluorescence-detection type, comprising: the step of pouring a sample into a gel in a migration unit, the step of mounting the migration unit in an electrophoresis unit, thereafter the step of performing electrophoresis of the sample, the step of thereafter removing the migration unit with the electrophoresed sample from the electrophoresis unit, the step of mounting the removed migration unit with the electrophoresed sample in a reading unit, and the step of reading the electrophoresis pattern of the electrophoresed sample in the migration unit by the reading unit.
Claim 21
Independent Claim
96 words
A method for reading an electrophoresis pattern of fluorescence-detection type, comprising: the step of pouring a sample into a gel in a migration unit, the step of mounting the migration unit in an electrophoresis unit, thereafter the step of performing electrophoresis of the sample, the step of thereafter removing the migration unit with the electrophoresed sample from the electrophoresis unit, the step of mounting the removed migration unit with the electrophoresed sample in a reading unit, and the step of reading the electrophoresis pattern of the electrophoresed sample in the migration unit by the reading unit.
Claim 22
Dependent Claim
64 words
A method for reading an electrophoresis pattern of fluorescent type as claimed in claim 19, further comprising the step of providing a plurality of the migration units and a plurality of the electrophoresis units, wherein the step of mounting the migration unit in the reading unit is carried out serially for each of the plurality of the migration units in a common reading unit.
Claim 23
Dependent Claim
75 words
A method for reading an electrophoresis pattern of fluorescence-detection type as claimed in claim 19, wherein a direction substantially parallel to a direction of electrophoresis is set as a first direction and a direction perpendicular to the direction of electrophoresis is set as a second direction, and the step of reading the electrophoresis pattern includes the step of defining a pixel size having a longer dimension in the first direction than in the second direction.
Claim 24
Dependent Claim
52 words
A method for reading an electrophoresis pattern of fluorescent type as claimed in claim 21, wherein the step of reading the electrophoresis pattern includes the step of obtaining data of a pattern of distribution of the fluorescent substance, and thinning out the data by at least one pixel in the second direction.
Claim 25
Dependent Claim
24 words
A method for reading an electrophoresis pattern as claimed in claim 19, wherein said step of pouring uses the sample comprises a nucleic acid.
Claim 26
Independent Claim
129 words
A method for reading an electrophoresis pattern of fluorescence-detection type, comprising: (a) pouring a plurality of samples one each into a like plurality of gels one each into a like plurality of migration units; (b) mounting each migration unit one each in a like plurality of electrophoresis units; (c) thereafter performing electrophoresis of each sample; (d) thereafter removing one migration unit with its electrophoresed sample from a first one of the plurality of electrophoresis units; (e) mounting the removed migration unit with its electrophoresed sample in a reading unit; (f) reading the electrophoresis pattern of the electrophoresis sample in the migration unit by the reading unit; and (g) repeating the steps (d) through (f) for each remaining electrophoresed sample, and using the same reading unit for each reading step.
Claim 27
Independent Claim
129 words
A method for reading an electrophoresis pattern of fluorescence-detection type, comprising: (a) pouring a plurality of samples one each into a like plurality of gels one each into a like plurality of migration units; (b) mounting each migration unit one each in a like plurality of electrophoresis units; (c) thereafter performing electrophoresis of each sample; (d) thereafter removing one migration unit with its electrophoresed sample from a first one of the plurality of electrophoresis units; (e) mounting the removed migration unit with its electrophoresed sample in a reading unit; (f) reading the electrophoresis pattern of the electrophoresis sample in the migration unit by the reading unit; and (g) repeating the steps (d) through (f) for each remaining electrophoresed sample, and using the same reading unit for each reading step.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-12-02 | 1995-06-03 | 1995-12-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-12-02 | 1999-06-03 | 1999-12-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-12-02 | 2003-06-03 | 2003-12-03 | $7700.00 | Window Closed / Lapsed |