Patent US5050937A successfully refreshed from Google Patents.
Patent US2132847A successfully refreshed from Google Patents.
Patent US5122244A successfully refreshed from Google Patents.
US5122244A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and sensor electrode system for the electrochemical determination of an analyte or an oxidoreductase as well as the use of suitable compounds therefor
Assignee: Roche Diagnostics GmbH
•
Filing: 1991-02-04
•
Granted: 1992-06-16
•
Expires: 2011-02-04
Abstract
Abstract The subject matter of the invention is a method for the electrochemical determination of an analyte in the presence of an oxidoreductase and a reducible substance which transfers electrons which arise during the course of the determination reaction from the oxidoreductase onto an electrode and thus leads to a signal which is a measure for the analyte to be determined whereby the reducible substance is enzymatically reduced and oxidized at the electrode, which is characterized in that the substance which forms at the electrode by oxidation is different from the reducible substance used initially, as well as a corresponding sensor electrode system and the use of compounds suitable therefor. Finally new nitrosoaniline derivatives and a process for their production are also subject matter of the invention.
Inventors
- 👤 Joachim Hoenes
- 👤 Jurgen Schaeffler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 21:00
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
96 words
A method for the electrochemical determination of an analyte in the presence of an oxidoreductase and a reducible substance which transfers electrons which arise during the course of the determination reaction from the oxidoreductase onto an electrode and thus leads to a signal which is a measure for the analyte to be determined whereby the reducible substance is enzymatically reduced and oxidized at the electrode in an overall irreversible reaction, wherein the substance which forms at the electrode by oxidation is an oxidized form of the enzymatically reduced substance other than the reducible substance used initially.
Claim 2
Independent Claim
96 words
A method for the electrochemical determination of an analyte in the presence of an oxidoreductase and a reducible substance which transfers electrons which arise during the course of the determination reaction from the oxidoreductase onto an electrode and thus leads to a signal which is a measure for the analyte to be determined whereby the reducible substance is enzymatically reduced and oxidized at the electrode in an overall irreversible reaction, wherein the substance which forms at the electrode by oxidation is an oxidized form of the enzymatically reduced substance other than the reducible substance used initially.
Claim 3
Dependent Claim
21 words
The method of claim 1, wherein said substance which forms at the electrode by oxidation is subsequently reduced by said oxido-reductase.
Claim 4
Dependent Claim
28 words
The method of claim 1, wherein said reducible substance accepts electrons arising during the course of the determination reaction from the oxidoreductase, thereby forming an electron-rich aromatic amine.
Claim 5
Dependent Claim
56 words
The method of claim 3, wherein said reducible substance is selected from the group of compounds of the formula I X--R (I) in which R represents an aromatic residue which is more electronegative than aniline, and X represents NO or NHOH, and compounds of the formula II HO--N═Y (II) in which Y represents a quinoid system.
Claim 6
Dependent Claim
22 words
The method of claim 1, wherein the oxidoreductase is selected from the group consisting of an oxidase, a non-NAD(P)-dependent dehydrogenase and diaphorase.
Claim 7
Independent Claim
92 words
A method for the electrochemical determination of an oxidoreductase in the presence of a corresponding enzyme substrate and a reducible substance which is capable of transferring electrons from the oxidoreductase onto an electrode and thus leads to a signal which is a measure for the enzyme to be determined, whereby the reducible substance is enzymatically reduced and oxidized at the electrode in an overall irreversible reaction, wherein the substance which forms by oxidation at the electrode is an oxidized form of the enzymatically reduced substance other than the reducible substance used initially.
Claim 8
Independent Claim
92 words
A method for the electrochemical determination of an oxidoreductase in the presence of a corresponding enzyme substrate and a reducible substance which is capable of transferring electrons from the oxidoreductase onto an electrode and thus leads to a signal which is a measure for the enzyme to be determined, whereby the reducible substance is enzymatically reduced and oxidized at the electrode in an overall irreversible reaction, wherein the substance which forms by oxidation at the electrode is an oxidized form of the enzymatically reduced substance other than the reducible substance used initially.
Claim 9
Independent Claim
110 words
A method for the electrochemical determination of an analyte, comprising contacting a sample containing said analyte with an oxidoreductase and a reducible substance which transfers electrons which are generated by the oxidation of said analyte onto an electrode; oxidizing said analyte with said oxidoreductase to produce a reduced enzyme; enzymatically reducing said reducible substance by said reduced enzyme to produce a reduced substance and regenerate said oxidoreductase, oxidizing said reduced substance at an electrode, thereby transferring electrons generated by the oxidation of said analyte to said electrode, wherein said oxidation of said reduced substance forms a substance which is different from said reducible substance, wherein the overall reaction is irreversible.
Claim 10
Independent Claim
110 words
A method for the electrochemical determination of an analyte, comprising contacting a sample containing said analyte with an oxidoreductase and a reducible substance which transfers electrons which are generated by the oxidation of said analyte onto an electrode; oxidizing said analyte with said oxidoreductase to produce a reduced enzyme; enzymatically reducing said reducible substance by said reduced enzyme to produce a reduced substance and regenerate said oxidoreductase, oxidizing said reduced substance at an electrode, thereby transferring electrons generated by the oxidation of said analyte to said electrode, wherein said oxidation of said reduced substance forms a substance which is different from said reducible substance, wherein the overall reaction is irreversible.
Claim 11
Dependent Claim
20 words
The method of claim 7, wherein said reducible substance is selected from the group consisting of N,N-bis-(2-hydroxyethyl) nitrosoaniline and N-(2-hydroxyethyl)-N-(2-(2-hydroxyethyoxy-4-nitrosoaniline.
Claim 12
Independent Claim
101 words
A method for the electrochemical determination of an oxidoreductase, comprising contacting a sample containing said oxidoreductase in the presence of a corresponding enzyme substrate with a reducible substance which transfers electrons which are generated by the oxidation of said enzyme substrate onto an electrode; enzymatically reducing said reducible substance by said oxidoreductase to produce a reduced substance, oxidizing said reduced substance at an electrode, thereby transferring electrons generated by the reduction of said reducible substance to said electrode, wherein said oxidation of said reduced substance forms a substance which is different from said reducible substance, wherein the overall reaction is irreversible.
Claim 13
Independent Claim
101 words
A method for the electrochemical determination of an oxidoreductase, comprising contacting a sample containing said oxidoreductase in the presence of a corresponding enzyme substrate with a reducible substance which transfers electrons which are generated by the oxidation of said enzyme substrate onto an electrode; enzymatically reducing said reducible substance by said oxidoreductase to produce a reduced substance, oxidizing said reduced substance at an electrode, thereby transferring electrons generated by the reduction of said reducible substance to said electrode, wherein said oxidation of said reduced substance forms a substance which is different from said reducible substance, wherein the overall reaction is irreversible.
References Cited (Backward Citations)
US4045297A
Cited
US4224125A
Cited
US4220503A
Cited
US4304853A
Cited
US4297173A
Cited
US4490464A
Cited
US4948727A
Cited
US4882013A
Cited
US4927516A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-06-16 | 1995-12-16 | 1996-06-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-06-16 | 1999-12-16 | 2000-06-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-06-16 | 2003-12-16 | 2004-06-16 | $7700.00 | Window Closed / Lapsed |