Patent US5151355A successfully refreshed from Google Patents.
US5151355A
Expired / Lapsed
US
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Process for the production of high purity thrombin
Assignee: Warner Lambert Pottery Road Ltd
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Filing: 1991-01-23
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Granted: 1992-09-29
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Expires: 2011-01-23
Abstract
Abstract A liquid thrombin preparation is prepared by reacting a unit of prothrombin with less than 5 units of thromboplastin in the presence of calcium, contacting the resultant thrombin with a phosphate buffer, and diluting and filtering the suspension. The filtrate is then applied sequentially to an anion-exchange agarose column and a cation-exchange agarose column and the thrombin fraction is step-wise eluted from the latter column with phosphate buffered saline. Liquid thrombin preparations thereby obtained have specific activities greater than 1,600 U/mg and can be used in haemostasis.
Inventors
- 👤 Carmel Crowley
- 👤 Timothy G. Sheehan
- 👤 Frederick G. Ward
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 09:23
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6429; A; A61; A61P; A61P7/00; A61P7/04; C12Y; C12Y304/00; C12Y304/21; C12Y304/21005
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
96 words
A process for the production of a liquid thrombin preparation, which process comprises reacting each unit of prothrombin measured as N.I.H. units of thrombin produced, with less than 5 units of thromboplastin, measured as N.I.H. units of thrombin produced, in the presence of calcium to produce thrombin contacting the resultant thrombin with a phosphate buffer to produce a suspension diluting and filtering the suspension to produce a filtrate applying the filtrate sequentially to an anion-exchange agarose column and a cation-exchange agarose column and step-wise eluting a thrombin fraction from the cation-exchange column with phosphate buffered saline.
Claim 2
Dependent Claim
96 words
A process for the production of a liquid thrombin preparation, which process comprises reacting each unit of prothrombin measured as N.I.H. units of thrombin produced, with less than 5 units of thromboplastin, measured as N.I.H. units of thrombin produced, in the presence of calcium to produce thrombin contacting the resultant thrombin with a phosphate buffer to produce a suspension diluting and filtering the suspension to produce a filtrate applying the filtrate sequentially to an anion-exchange agarose column and a cation-exchange agarose column and step-wise eluting a thrombin fraction from the cation-exchange column with phosphate buffered saline.
Claim 3
Dependent Claim
18 words
A process according to claim 1, wherein the amount of thromboplastin used is between 2 and 4 units.
Claim 4
Dependent Claim
19 words
A process according to claim 1, wherein the amount of thromboplastin used is of the order of 3 units.
Claim 5
Dependent Claim
19 words
A process according to claim 1, wherein the phosphate buffer used is a 0.5-1.5M phosphate buffer at pH 6.0-7.0.
Claim 6
Dependent Claim
19 words
A process according to claim 1, wherein the phosphate buffer used is a 1.0M phosphate buffer at pH 6.5.
Claim 7
Dependent Claim
20 words
A process according to claim 1, wherein the step-wise elution of the cation exchange agarose column is a two-step elution.
Claim 8
Dependent Claim
27 words
A process according to claim 1, wherein the cation exchange agarose column is sequentially eluted with i) 0.16-0.18M phosphate buffered saline and ii) 0.26-0.30M phosphate buffered saline.
Claim 9
Dependent Claim
27 words
A process according to claim 1, wherein the cation exchange agarose column is sequentially eluted with i) 0.17M phosphate buffered saline and ii) 0.28M phosphate buffered saline.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-09-29 | 1996-03-30 | 1996-09-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-09-29 | 2000-03-30 | 2000-09-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-09-29 | 2004-03-30 | 2004-09-29 | $7700.00 | Window Closed / Lapsed |