US5208154A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Reversibly immobilized biological materials in monolayer films on electrodes
Assignee: United States Department of Energy
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Filing: 1991-04-08
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Granted: 1993-05-04
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Expires: 2011-04-08
Abstract
Abstract Methods and techniques are described for reversibly binding charged biological particles in a fluid medium to an electrode surface. The methods are useful in a variety of applications. The biological materials may include microbes, proteins, and viruses. The electrode surface may consist of reversibly electroactive materials such as polyvinylferrocene, silicon-linked ferrocene or quinone.
Inventors
- 👤 Paul F. Weaver
- 👤 Arthur J. Frank
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 08:41
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815; Y10S435/817
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
105 words
A method for reversibly binding negatively charged biological particles in a liquid medium at neutral pH to an electrode surface, wherein said particles are selected from the group consisting of microbial cells and proteins the method comprising the steps of: (a) treating said electrode surface with an electrochemically active material selected from the group consisting of polyvinylferrocene, silicon-linked ferrocene and quinone; (b) connecting said electrode to an electrical potential; wherein said electrical potential induces a positive charge on said electrochemically active material; and (c) exposing said medium to said electrode surface in a manner such that said charged particles are adsorbed on said electrode surface.
Claim 2
Independent Claim
105 words
A method for reversibly binding negatively charged biological particles in a liquid medium at neutral pH to an electrode surface, wherein said particles are selected from the group consisting of microbial cells and proteins the method comprising the steps of: (a) treating said electrode surface with an electrochemically active material selected from the group consisting of polyvinylferrocene, silicon-linked ferrocene and quinone; (b) connecting said electrode to an electrical potential; wherein said electrical potential induces a positive charge on said electrochemically active material; and (c) exposing said medium to said electrode surface in a manner such that said charged particles are adsorbed on said electrode surface.
Claim 3
Dependent Claim
26 words
A method in accordance with claim 1, comprising the further step of changing said electrical potential to cause release of said particles from said electrode surface.
Claim 4
Dependent Claim
12 words
A method in accordance with claim 1, wherein said liquid comprises water.
Claim 5
Dependent Claim
13 words
A method in accordance with claim 1, wherein said electrode surface is planar.
Claim 6
Dependent Claim
17 words
A method in accordance with claim 1, wherein said electroactive material consists of polyvinylferrocene or silicon-linked ferrocene.
Claim 7
Dependent Claim
29 words
A method in accordance with claim 1, wherein said electrode surface is made of a material that is selected from the group consisting of tin oxide-coated glass and carbon.
Claim 8
Dependent Claim
13 words
A method in accordance with claim 3, wherein said charged particles comprise proteins.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-05-03 | 1996-11-02 | 1997-05-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-05-03 | 2000-11-02 | 2001-05-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-05-03 | 2004-11-02 | 2005-05-04 | $7700.00 | Window Closed / Lapsed |