Patent US5208154A successfully refreshed from Google Patents.
US5208154A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Reversibly immobilized biological materials in monolayer films on electrodes
Assignee: United States Department of Energy
•
Filing: 1991-04-08
•
Granted: 1993-05-04
•
Expires: 2011-04-08
Abstract
Abstract Methods and techniques are described for reversibly binding charged biological particles in a fluid medium to an electrode surface. The methods are useful in a variety of applications. The biological materials may include microbes, proteins, and viruses. The electrode surface may consist of reversibly electroactive materials such as polyvinylferrocene, silicon-linked ferrocene or quinone.
Inventors
- 👤 Paul F. Weaver
- 👤 Arthur J. Frank
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 20:58
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815; Y10S435/817
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
105 words
A method for reversibly binding negatively charged biological particles in a liquid medium at neutral pH to an electrode surface, wherein said particles are selected from the group consisting of microbial cells and proteins the method comprising the steps of: (a) treating said electrode surface with an electrochemically active material selected from the group consisting of polyvinylferrocene, silicon-linked ferrocene and quinone; (b) connecting said electrode to an electrical potential; wherein said electrical potential induces a positive charge on said electrochemically active material; and (c) exposing said medium to said electrode surface in a manner such that said charged particles are adsorbed on said electrode surface.
Claim 2
Independent Claim
105 words
A method for reversibly binding negatively charged biological particles in a liquid medium at neutral pH to an electrode surface, wherein said particles are selected from the group consisting of microbial cells and proteins the method comprising the steps of: (a) treating said electrode surface with an electrochemically active material selected from the group consisting of polyvinylferrocene, silicon-linked ferrocene and quinone; (b) connecting said electrode to an electrical potential; wherein said electrical potential induces a positive charge on said electrochemically active material; and (c) exposing said medium to said electrode surface in a manner such that said charged particles are adsorbed on said electrode surface.
Claim 3
Dependent Claim
26 words
A method in accordance with claim 1, comprising the further step of changing said electrical potential to cause release of said particles from said electrode surface.
Claim 4
Dependent Claim
12 words
A method in accordance with claim 1, wherein said liquid comprises water.
Claim 5
Dependent Claim
13 words
A method in accordance with claim 1, wherein said electrode surface is planar.
Claim 6
Dependent Claim
17 words
A method in accordance with claim 1, wherein said electroactive material consists of polyvinylferrocene or silicon-linked ferrocene.
Claim 7
Dependent Claim
29 words
A method in accordance with claim 1, wherein said electrode surface is made of a material that is selected from the group consisting of tin oxide-coated glass and carbon.
Claim 8
Dependent Claim
13 words
A method in accordance with claim 3, wherein said charged particles comprise proteins.
References Cited (Backward Citations)
US3839175A
Cited
US4563425A
Cited
US4545382A
Cited
US4581336A
Cited
US4438196A
Cited
US4572897A
Cited
US4711245A
Cited
US4798801A
Cited
US4585652A
Cited
US4832797A
Cited
US4857167A
Cited
US4953552A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-05-03 | 1996-11-02 | 1997-05-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-05-03 | 2000-11-02 | 2001-05-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-05-03 | 2004-11-02 | 2005-05-04 | $7700.00 | Window Closed / Lapsed |