Patent US5284933A successfully refreshed from Google Patents.
US5284933A
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Affinity peptides
Assignee: Roche Diagnostics Corp
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Filing: 1988-02-22
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Granted: 1994-02-08
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Expires: 2008-02-22
Abstract
Abstract Fusion proteins and a process for their purification by means of metal chelate affinity chromatography on NTA resins are provided by this invention.
Inventors
- 👤 Heinz Dobeli
- 👤 Bernhard Eggimann
- 👤 Reiner Gentz
- 👤 Erich Hochuli
- 👤 Dietrich Stuber
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 21:50
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/57; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/62; C12N15/63; C12N15/70; A; A61; A61K; A61K38/00; C07K2319/00; C07K2319/20; C07K2319/21; C07K2319/50; C07K2319/70; C07K2319/74; C07K2319/75
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
76 words
A fusion protein comprising a biologically active polypeptide or protein linked by its amino- and/or carboxyl-terminus to one or two affinity peptides, which affinity peptides have the formula R.sup.1 --(His).sub.2-6 --R.sup.2, wherein R 1 is hydrogen or a peptide chain formed from about 1 to about 30 amino acids; and R 2 is Q, Q-Ile-Glu-Gly-Arg- or Q-Asp-Asp-Asp-Asp-Lys, where Q is a peptide bond or a peptide chain formed from about 1 to about 30 amino acids.
Claim 2
Dependent Claim
76 words
A fusion protein comprising a biologically active polypeptide or protein linked by its amino- and/or carboxyl-terminus to one or two affinity peptides, which affinity peptides have the formula R.sup.1 --(His).sub.2-6 --R.sup.2, wherein R 1 is hydrogen or a peptide chain formed from about 1 to about 30 amino acids; and R 2 is Q, Q-Ile-Glu-Gly-Arg- or Q-Asp-Asp-Asp-Asp-Lys, where Q is a peptide bond or a peptide chain formed from about 1 to about 30 amino acids.
Claim 3
Dependent Claim
23 words
The fusion protein of claim 1 in which the affinity peptides have an amino acid sequence selected from the group consisting of ##STR3##
Claim 4
Dependent Claim
31 words
The fusion protein of claim 1 in which at least one of the links between the biologically active polypeptide or protein and the affinity peptides can be cleaved by a protease.
Claim 5
Dependent Claim
12 words
The fusion protein of claim 3 in which the protease is enterokinase.
Claim 6
Dependent Claim
16 words
The fusion protein of claim 3 in which the protease is coagulation factor X a .
Claim 7
Dependent Claim
15 words
The fusion protein of claim 1 in which the affinity peptides complex immobilized nickel ions.
Claim 8
Dependent Claim
37 words
The fusion protein of claim 1 in which the biologically active polypeptide or protein has an amino acid sequence corresponding to the sequence of human immune interferon, a subsequence of human immune interferon, or mouse dihydrofolate reductase.
Claim 9
Dependent Claim
10 words
The fusion protein of claim 1 which is essentially pure.
Claim 10
Dependent Claim
53 words
An affinity peptide of formula R.sup.1 --(His).sub.2-6 --R.sup.2, wherein R 1 is hydrogen or a peptide chain formed from about 1 to about 30 amino acids; and R 2 is Q, Q-Ile-Glu-Gly-Arg- or Q-Asp-Asp-Asp-Asp-Lys-, where Q is a peptide bond or a peptide chain formed from about 1 to about 30 amino acids.
Claim 11
Dependent Claim
53 words
An affinity peptide of formula R.sup.1 --(His).sub.2-6 --R.sup.2, wherein R 1 is hydrogen or a peptide chain formed from about 1 to about 30 amino acids; and R 2 is Q, Q-Ile-Glu-Gly-Arg- or Q-Asp-Asp-Asp-Asp-Lys-, where Q is a peptide bond or a peptide chain formed from about 1 to about 30 amino acids.
Claim 12
Dependent Claim
18 words
The affinity peptide of claim 9 having an amino acid sequence selected from the group consisting of ##STR4##
References Cited (Backward Citations)
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