Patent US5344754A successfully refreshed from Google Patents.
US5344754A
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Assay timed by electrical resistance change and test strip
Assignee: Beckman Coulter Inc
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Filing: 1993-01-13
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Granted: 1994-09-06
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Expires: 2013-01-13
Abstract
Abstract Assays for liquid analytes are performed on a bibulous matrix containing dried reagents which produce a visibly detectable reaction product. Application of liquid sample to the bibulous matrix is detected by measuring a drop in resistance across the matrix. A preferred test article for performing the method includes the matrix and a pair of spaced-apart electrodes in electrical contact with a reaction zone on the matrix. The test article is used in combination with a detection unit having means for probing the electrodes to determine when electrical resistance in the matrix has decreased. The assay methods and apparatus are particularly useful for performing enzyme assays where signal developed as a function of time is critical.
Inventors
- 👤 Stephen E. Zweig
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 06:17
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/02; G01N27/04; G01N27/041; C; C12; C12Q; C12Q1/00; C12Q1/56; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808; Y10T; Y10T436/00; Y10T436/11; Y10T436/112499
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
106 words
An assay for determining an analyte in a liquid sample, said assay comprising: applying a volume of the sample to a target location on a bibulous matrix, wherein the matrix comprises one or more dried reagents which in the presence of the analyte initiate time-dependent production of an optically detectable signal within the matrix; measuring a change in electrical resistance across the target location which results from the application of liquid sample; initiating a timing cycle when a lowering of electrical resistance is first measured; and measuring the production of said optically detectable signal within the matrix at one or more times during the timing cycle.
Claim 2
Dependent Claim
106 words
An assay for determining an analyte in a liquid sample, said assay comprising: applying a volume of the sample to a target location on a bibulous matrix, wherein the matrix comprises one or more dried reagents which in the presence of the analyte initiate time-dependent production of an optically detectable signal within the matrix; measuring a change in electrical resistance across the target location which results from the application of liquid sample; initiating a timing cycle when a lowering of electrical resistance is first measured; and measuring the production of said optically detectable signal within the matrix at one or more times during the timing cycle.
Claim 3
Dependent Claim
17 words
An assay as in claim 1 wherein the sample volume is from 3 μl to 50 μl.
Claim 4
Dependent Claim
13 words
An assay as in claim 1, wherein the sample is a patient sample.
Claim 5
Dependent Claim
12 words
An assay as in claim 3, wherein the patient sample is blood.
Claim 6
Dependent Claim
30 words
An assay as in claim 1, wherein the analyte is an enzyme and the reagents include an enzyme substrate which produces an optically detectable change after exposure to the enzyme.
Claim 7
Dependent Claim
17 words
An assay as in claim 5 wherein the optically detectable change is fluorescence, luminescence, or color change.
Claim 8
Dependent Claim
29 words
An assay as in claim 5, wherein the analyte is a component of a coagulation pathway and the enzyme substrate produces the detectable signal upon activation by said component.
Claim 9
Dependent Claim
18 words
An assay as in claim 1, further comprising controlling the temperature of the bibulous matrix to 37° C.
Claim 10
Dependent Claim
25 words
An assay as in claim 1, further comprising continuously monitoring the electrical resistance across the target location, whereby loss of liquid sample can be detected.
Claim 11
Dependent Claim
24 words
An assay as in claim 1, wherein the production of signal is detected at a plurality of time points over a preselected time duration.
Claim 12
Dependent Claim
74 words
An improved enzyme assay of the type wherein a liquid sample suspected of containing the enzyme is applied to a bibulous matrix-containing reagents which result in production of an optically detectable signal over time, wherein the improvement comprises measuring a change in electrical resistance within the matrix resulting from application of the liquid sample and optically detecting the production of signal at one or more times after the electrical resistance change is first measured.
Claim 13
Dependent Claim
74 words
An improved enzyme assay of the type wherein a liquid sample suspected of containing the enzyme is applied to a bibulous matrix-containing reagents which result in production of an optically detectable signal over time, wherein the improvement comprises measuring a change in electrical resistance within the matrix resulting from application of the liquid sample and optically detecting the production of signal at one or more times after the electrical resistance change is first measured.
Claim 14
Dependent Claim
36 words
An improved assay as in claim 11, wherein electrical resistance is measured by contacting a pair of electrodes which are secured to the bibulous matrix on either side of a location where liquid sample is applied.
Claim 15
Dependent Claim
28 words
An improved assay as in claim 11, wherein the electrical resistance is continuously monitored to assure that the liquid sample has not been lost during the assay procedure.
Claim 16
Dependent Claim
36 words
An improved assay as in claim 11, wherein the production of signal is detected at a plurality of time points over a preselected time duration which commences when the change in electrical resistance is first measured.
Claim 17
Dependent Claim
76 words
A test article comprising: a bibulous matrix; one or more dried reagents which are present within the matrix and which, when wetted by a liquid sample, initiate an optically detectable chemical reaction with an analyte present in the sample; and a pair of spaced-apart electrodes proximate a sample target location of the matrix, wherein the electrodes are disposed so that application of the liquid sample to the target location will lower electrical resistance between the electrodes.
Claim 18
Dependent Claim
76 words
A test article comprising: a bibulous matrix; one or more dried reagents which are present within the matrix and which, when wetted by a liquid sample, initiate an optically detectable chemical reaction with an analyte present in the sample; and a pair of spaced-apart electrodes proximate a sample target location of the matrix, wherein the electrodes are disposed so that application of the liquid sample to the target location will lower electrical resistance between the electrodes.
Claim 19
Dependent Claim
39 words
A test article as in claim 15, wherein the spaced-apart electrodes are secured to the bibulous matrix and in electrical contact with at least a portion of a surface of the matrix on opposite sides of the target location.
Claim 20
Dependent Claim
24 words
A test article as in claim 16, wherein the electrodes are metal foil strips which are attached to the matrix surface by an adhesive.
Claim 21
Dependent Claim
36 words
A test article as in claim 15, wherein the gap between the spaced-apart electrodes has a width in the range from 0.1 mm to 2 mm, whereby the electrode inhibits evaporation of sample from the matrix.
Claim 22
Dependent Claim
24 words
A test article as in claim 18, further comprising a transparent support element attached to a surface of the matrix opposite to the electrodes.
Claim 23
Dependent Claim
27 words
A test article as in claim 15, wherein the bibulous matrix is composed of a hydrophilic, non-swellable material which is free from interference with a coagulation pathway.
Claim 24
Dependent Claim
31 words
A test article as in claim 20, wherein the reagents comprise a coagulation initiator and a substrate which produces a detectable signal upon activation by a component of the coagulation pathway.
Claim 25
Dependent Claim
41 words
A test article as in claim 21, wherein the coagulation pathway component is thrombin and the substrate is a peptide cleavably bound to a reporter molecule, wherein thrombin binds the peptide and cleaves the reporter molecule to produce the detectable signal.
Claim 26
Dependent Claim
13 words
A test article as in claim 22, wherein the detectable signal is fluorescence.
Claim 27
Dependent Claim
79 words
A test article comprising: a bibulous matrix; one or more dried reagents which are dispersed within the matrix and which, when wetted by a liquid sample, initiate an optically detectable enzymatic reaction with an enzyme analyte present in the sample; and a pair of spaced-apart metal strips attached to a surface of the matrix to define a sample target location therebetween, whereby application of the liquid sample to the target location will lower electrical resistance between the metal strips.
Claim 28
Dependent Claim
79 words
A test article comprising: a bibulous matrix; one or more dried reagents which are dispersed within the matrix and which, when wetted by a liquid sample, initiate an optically detectable enzymatic reaction with an enzyme analyte present in the sample; and a pair of spaced-apart metal strips attached to a surface of the matrix to define a sample target location therebetween, whereby application of the liquid sample to the target location will lower electrical resistance between the metal strips.
Claim 29
Dependent Claim
20 words
A test article as in claim 24, where the metal strips are attached to the matrix surface by an adhesive.
Claim 30
Dependent Claim
37 words
A test article as in claim 24, wherein the gap between the spaced-apart metal strips has a width in the range from 0.1 mm to 2 mm, whereby the strips inhibit evaporation of sample from the matrix.
Claim 31
Dependent Claim
25 words
A test article as in claim 26, further comprising a transparent support element attached to a surface of the membrane opposite to the metal strips.
Claim 32
Dependent Claim
27 words
A test article as in claim 24, wherein the bibulous matrix is composed of a hydrophilic, nonswellable material which is free from interference with a coagulation pathway.
Claim 33
Dependent Claim
31 words
A test article as in claim 28, wherein the reagents comprise a coagulation initiator and a substrate which produces a detectable signal upon activation by a component of the coagulation pathway.
Claim 34
Dependent Claim
40 words
A test article as in claim 29, wherein the coagulation initiation is thrombin and the substrate is a peptide cleavably bound to a reporter molecule, wherein thrombin binds the peptide and cleaves the reporter molecule to produce the detectable signal.
Claim 35
Dependent Claim
13 words
A test article as in claim 30, wherein the detectable signal is fluorescence.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-09-05 | 1998-03-07 | 1998-09-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-09-05 | 2002-03-07 | 2002-09-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-09-05 | 2006-03-07 | 2006-09-06 | $7700.00 | Window Closed / Lapsed |