US5354692A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Analyte detection device including a hydrophobic barrier for improved fluid flow
Assignee: Quidel Corp
•
Filing: 1992-09-08
•
Granted: 1994-10-11
•
Expires: 2012-09-08
Abstract
Abstract An immunochromatographic assay device for the detection of an analyte in a liquid sample, the device including a housing that contains an opening for the application of the liquid sample and multiple liquid permeable materials located in the housing that are adapted to receive, treat and facilitate the movement of the sample through the housing; preferably, a first liquid permeable material is adapted to receive the liquid sample; a second liquid permeable material is positioned under the first liquid permeable material; a third liquid permeable material is positioned above the second liquid material; means are provided to prevent liquid communication between the first and third liquid permeable materials; a wicking material, in fluid contact with the third liquid permeable material receives the sample; and reagents are positioned in the housing that display the results of the immunoassay.
Inventors
- 👤 Hsin M. Yang
- 👤 Michael Newton
- 👤 Ping Liu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 11:07
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54388; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/97; Y10S436/00; Y10S436/805; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
236 words
An immunochromatographic assay device for detection of an analyte comprising: a housing having an opening for introduction of a liquid sample; first liquid permeable material in said housing under said opening to receive a liquid sample introduced through said opening and defining a sample receiving zone; second liquid permeable material in said housing under said first liquid permeable material in fluid contact with said first liquid permeable material to receive sample from said first liquid permeable material, said second liquid permeable material being absorbent and defining a sample transport zone; third liquid permeable material in said housing adjacent said first liquid permeable material and in fluid contact with said second permeable material to receive sample from said second liquid permeable material, said third liquid permeable located on top of said second liquid permeable material; a barrier comprising a hydrophobic material interposed between said first and third liquid permeable materials to prevent direct transfer of sample from said first liquid permeable material to said third liquid permeable material; an elongated sheet of wicking material in fluid contact with said third liquid permeable material in fluid contact with said third liquid permeable material to receive sample therefrom, wherein said elongated sheet of wicking material supports an immobilized capture molecule for said analyte; and a detecting reagent located in one of said liquid permeable materials to detect the presence of bound analyte in said elongated sheet of wicking material.
Claim 2
Dependent Claim
236 words
An immunochromatographic assay device for detection of an analyte comprising: a housing having an opening for introduction of a liquid sample; first liquid permeable material in said housing under said opening to receive a liquid sample introduced through said opening and defining a sample receiving zone; second liquid permeable material in said housing under said first liquid permeable material in fluid contact with said first liquid permeable material to receive sample from said first liquid permeable material, said second liquid permeable material being absorbent and defining a sample transport zone; third liquid permeable material in said housing adjacent said first liquid permeable material and in fluid contact with said second permeable material to receive sample from said second liquid permeable material, said third liquid permeable located on top of said second liquid permeable material; a barrier comprising a hydrophobic material interposed between said first and third liquid permeable materials to prevent direct transfer of sample from said first liquid permeable material to said third liquid permeable material; an elongated sheet of wicking material in fluid contact with said third liquid permeable material in fluid contact with said third liquid permeable material to receive sample therefrom, wherein said elongated sheet of wicking material supports an immobilized capture molecule for said analyte; and a detecting reagent located in one of said liquid permeable materials to detect the presence of bound analyte in said elongated sheet of wicking material.
Claim 3
Dependent Claim
35 words
The assay device of claim 1, wherein said first and third liquid permeable materials and said hydrophobic material are physically joined together into an integral sheet which is separate from said second liquid permeable material.
Claim 4
Dependent Claim
16 words
The assay device of claim 1, wherein said first liquid permeable material is porous thermoplastic polymer.
Claim 5
Dependent Claim
16 words
The assay device of claim 3, wherein said third liquid permeable material is porous thermoplastic polymer.
Claim 6
Dependent Claim
15 words
The assay device of claim 4, wherein said porous thermoplastic polymer is high density polyethylene.
Claim 7
Dependent Claim
29 words
The assay device of claim 4, wherein said first liquid permeable material has a pore size that is smaller than the pore size of said third liquid permeable material.
Claim 8
Dependent Claim
29 words
The assay device of claim 1, wherein said first liquid permeable material has a pore size of not greater than about 300 microns and acts to filter said sample.
Claim 9
Dependent Claim
42 words
The assay device of claim 1, further comprising an absorbent material in fluid contact with an end of said wicking material which is distal from the end of said wicking material which is in fluid contact with said third liquid permeable material.
Claim 10
Dependent Claim
31 words
The assay device of claim 1, wherein said first liquid permeable material has a sufficient pore size to filter said liquid sample or contains a reagent to pretreat said liquid sample.
Claim 11
Dependent Claim
178 words
An immunocromatographic assay device for detection of an analyte in a liquid sample comprising a housing, a sample receiving zone in said housing, an elongated sheet of wicking material in fluid communication with said sample receiving zone, wherein said elongated sheet of wicking material supports an immobilized capture molecule for said analyte, and a detecting reagent located in said sample receiving zone to detect the presence of bound analyte in said elongated sheet of wicking material, said sample receiving zone comprising a sample treatment pad having a first liquid permeable material for receiving the liquid sample, a second liquid permeable material in fluid communication with said first liquid permeable material in order to receive sample from said first liquid permeable material, and a third liquid permeable material in fluid communication with said second liquid permeable material and adjacent said first liquid permeable material, wherein a barrier comprising a hydrophobic material is interposed between said first and third liquid permeable materials to prevent direct transfer of sample from said first liquid permeable material to said third liquid permeable material.
Claim 12
Dependent Claim
178 words
An immunocromatographic assay device for detection of an analyte in a liquid sample comprising a housing, a sample receiving zone in said housing, an elongated sheet of wicking material in fluid communication with said sample receiving zone, wherein said elongated sheet of wicking material supports an immobilized capture molecule for said analyte, and a detecting reagent located in said sample receiving zone to detect the presence of bound analyte in said elongated sheet of wicking material, said sample receiving zone comprising a sample treatment pad having a first liquid permeable material for receiving the liquid sample, a second liquid permeable material in fluid communication with said first liquid permeable material in order to receive sample from said first liquid permeable material, and a third liquid permeable material in fluid communication with said second liquid permeable material and adjacent said first liquid permeable material, wherein a barrier comprising a hydrophobic material is interposed between said first and third liquid permeable materials to prevent direct transfer of sample from said first liquid permeable material to said third liquid permeable material.
Claim 13
Dependent Claim
31 words
The assay device of claim 10, wherein said first liquid permeable material has a sufficient pore size to filter said liquid sample or contains a reagent to pretreat said liquid sample.
Claim 14
Dependent Claim
68 words
The assay device of claim 9, wherein said liquid sample is pretreated with immobilized antibody in said first liquid permeable material from an animal immunized with a control fluid sample from a human, said control fluid sample comprising a negative control specimen, wherein said control fluid sample does not contain said analyte, said control fluid sample thereby testing negative in an assay performed with said immunochromatographic assay device.
Claim 15
Dependent Claim
16 words
The assay device of claim 10, wherein said liquid permeable material is a porous thermoplastic polymer.
Claim 16
Dependent Claim
15 words
The assay device of claim 10, wherein said hydrophobic means is polypropylene hot melt adhesive.
Claim 17
Dependent Claim
15 words
The assay device of claim 10, wherein said hydrophobic means is a liquid-impermeable thermoplastic polymer.
Claim 18
Dependent Claim
19 words
The assay device of claim 10, wherein a further improvement comprises means for preventing flooding in said assay device.
Claim 19
Dependent Claim
30 words
The assay device of claim 16, wherein said means for preventing flooding is a divider that controls the flow of liquid from said liquid permeable materials to said wicking material.
Claim 20
Dependent Claim
27 words
The assay device of claim 17, wherein said means for preventing flooding flow includes a depression adjacent to said divider to collect liquid pooling in the housing.
Claim 21
Dependent Claim
45 words
The assay device of claim 10, wherein said housing additionally comprises at least two support rails extending in the direction of fluid flow for supporting said wicking material, said support rails defining at least one recess between said support rails wherein excess liquid can pool.
Claim 22
Dependent Claim
31 words
The assay device of claim 10, wherein said third liquid permeable material has a sufficient pore size to filter said liquid sample or contains a reagent to pretreat said liquid sample.
Claim 23
Dependent Claim
123 words
A sample treatment pad for use in an immunochromatographic assay device comprising at least a first liquid permeable material; a second liquid permeable material in fluid contact with said first liquid permeable material in order to receive sample from said first liquid permeable material, said second liquid permeable material being absorbent and defining a sample transport zone; a third liquid permeable material adjacent said first liquid permeable material and in fluid contact with said second liquid permeable material in order to receive sample from said second liquid permeable material; and a barrier comprising a hydrophobic material interposed between said first and third liquid permeable materials to prevent direct transfer of sample from said first liquid permeable material to said third liquid permeable material.
Claim 24
Dependent Claim
123 words
A sample treatment pad for use in an immunochromatographic assay device comprising at least a first liquid permeable material; a second liquid permeable material in fluid contact with said first liquid permeable material in order to receive sample from said first liquid permeable material, said second liquid permeable material being absorbent and defining a sample transport zone; a third liquid permeable material adjacent said first liquid permeable material and in fluid contact with said second liquid permeable material in order to receive sample from said second liquid permeable material; and a barrier comprising a hydrophobic material interposed between said first and third liquid permeable materials to prevent direct transfer of sample from said first liquid permeable material to said third liquid permeable material.
Claim 25
Dependent Claim
21 words
The sample treatment pad of claim 21, wherein at least one of said liquid permeable materials is a porous thermoplastic polymer.
Claim 26
Dependent Claim
16 words
The sample treatment pad of claim 21, wherein said hydrophobic barrier is polypropylene, silicone, or rubber.
Claim 27
Dependent Claim
15 words
The sample treatment pad of claim 21, wherein said hydrophobic barrier is high density polyethylene.
Claim 28
Dependent Claim
21 words
The sample treatment pad of claim 21, wherein at least one of said liquid permeable materials is a glass fiber pad.
References Cited (Backward Citations)
US3011874A
Cited
US3211645A
Cited
US3551555A
Cited
US3420205A
Cited
US3811840A
Cited
US3691017A
Cited
US3850752A
Cited
US4190496A
Cited
US3888629A
Cited
US3895914A
Cited
US3966897A
Cited
US3902847A
Cited
US4138474A
Cited
US3961899A
Cited
US4169138A
Cited
US3915647A
Cited
US3996006A
Cited
US3979509A
Cited
US4001583A
Cited
US4017261A
Cited
US4168146A
Cited
US3990849A
Cited
US4039552A
Cited
US4125372A
Cited
US4180383A
Cited
US4092408A
Cited
US4317810A
Cited
US4067959A
Cited
US4225784A
Cited
US4094647A
Cited
US4185084A
Cited
US4248965A
Cited
US4205058A
Cited
US4225557A
Cited
US4208479A
Cited
US4233402A
Cited
US4193983A
Cited
US4212742A
Cited
US4256693A
Cited
US4230683A
Cited
US4302536A
Cited
US4277560A
Cited
US4298687A
Cited
US4246339A
Cited
US4235601A
Cited
US4278653A
Cited
US4305924A
Cited
US4843000A
Cited
US4391904A
Cited
US4373932A
Cited
US4360358A
Cited
US4361647A
Cited
US4301249A
Cited
US4376110A
Cited
US4366241A
Cited
US4366241B1
Cited
US4425438A
Cited
US4533629A
Cited
US4447529A
Cited
US4472498A
Cited
US4461829A
Cited
US4582792A
Cited
US4849338A
Cited
US4424279A
Cited
US4704255A
Cited
US4552839A
Cited
US4637978A
Cited
US4594327A
Cited
US4595661A
Cited
US4690907A
Cited
US4703017C1
Cited
US4703017A
Cited
US4672024A
Cited
US4727019A
Cited
US4999285A
Cited
US4861711A
Cited
US4740468A
Cited
US4649121A
Cited
US4623461A
Cited
US4780280A
Cited
US4670381A
Cited
US4618475A
Cited
EP0217403A2
Cited
US4837168A
Cited
US4916056A
Cited
US4960691A
Cited
US4774192A
Cited
US4857453A
Cited
US4855240A
Cited
US4943522A
Cited
US4962023A
Cited
US5120643A
Cited
US5073344A
Cited
US4981786A
Cited
US4912034A
Cited
EP0323605B1
Cited
GB8808534D0
Cited
EP0342913A2
Cited
US5202268A
Cited
EP0421294A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-10-10 | 1998-04-11 | 1998-10-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-10-10 | 2002-04-11 | 2002-10-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-10-10 | 2006-04-11 | 2006-10-11 | $7700.00 | Window Closed / Lapsed |