US5490937A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Tangential flow filtration process and apparatus
Assignee: Genentech Inc
•
Filing: 1994-07-06
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Granted: 1996-02-13
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Expires: 2014-07-06
Abstract
Abstract Processes and apparati are provided for separating species of interest from a mixture containing them which comprises subjecting the mixture to tangential-flow filtration, wherein the filtration membrane preferably has a pore size that retains species with a size up to about 10 microns, and the flux is maintained at a level ranging from about 5 % up to 100 % of transition point flux.
Inventors
- 👤 Robert D. van Reis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 01:51
CPC Classifications
B; B01; B01D; B01D61/00; B01D61/14; B01D61/145; B01D61/146; B01D61/147; C; C07; C07K; C07K1/00; C07K1/14; C07K1/34; C12; C12M; C12M23/00; C12M23/58; C12M47/00; C12M47/02; C12M47/10
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
96 words
A process for separating species of interest having a molecular weight of about 1 to 1000 kDa from a mixture, which process comprises filtering the mixture by tangential-flow filtration through a filtration membrane having a pore size that separates said species of interest from the mixture, while maintaining flux at a level ranging from about 5 to 100% of transition point flux in the pressure-dependent region of the flux versus TMP curve, whereby the species of interest are selectively separated from the mixture, and wherein transmembrane pressure decreases or remains approximately constant as the filtration progresses.
Claim 2
Dependent Claim
96 words
A process for separating species of interest having a molecular weight of about 1 to 1000 kDa from a mixture, which process comprises filtering the mixture by tangential-flow filtration through a filtration membrane having a pore size that separates said species of interest from the mixture, while maintaining flux at a level ranging from about 5 to 100% of transition point flux in the pressure-dependent region of the flux versus TMP curve, whereby the species of interest are selectively separated from the mixture, and wherein transmembrane pressure decreases or remains approximately constant as the filtration progresses.
Claim 3
Dependent Claim
19 words
The process of claim 1 wherein the flux is from about 50 to 100% of the transition point flux.
Claim 4
Dependent Claim
19 words
The process of claim 1 wherein the flux is from about 75 to 100% of the transition point flux.
Claim 5
Dependent Claim
10 words
The process of claim 1 wherein the mixture is undiluted.
Claim 6
Dependent Claim
26 words
The process of claim l wherein retentate from the filtration is recycled to a tank containing the mixture to be separated and the process is repeated.
Claim 7
Dependent Claim
13 words
The process of claim 1 wherein transmembrane pressure decreases as the filtration progresses.
Claim 8
Dependent Claim
15 words
The process of claim 1 wherein transmembrane pressure remains approximately constant as the filtration progresses.
Claim 9
Dependent Claim
31 words
The process of claim 1 wherein the species of interest are biological entities selected from the group consisting of proteins, polypeptides, amino acids, colloids, mycoplasm, endotoxins, viruses, carbohydrates, RNA, and DNA.
Claim 10
Dependent Claim
14 words
The process of claim 8 wherein the species of interest are proteins or polypeptides.
Claim 11
Dependent Claim
30 words
The process of claim 1 wherein the species of interest are less than ten times larger or smaller in molecular weight than the other species of interest of the mixture.
Claim 12
Dependent Claim
31 words
The process of claim 1 wherein transmembrane pressure is held substantially constant along the membrane at a level no greater than the transmembrane pressure at the transition point of the filtration.
Claim 13
Dependent Claim
112 words
A process for separating at least two proteins or polypeptides from each other that are contained in a mixture, wherein the proteins or polypeptides differ from each other in molecular weight by less than ten fold, which process comprises filtering the mixture by tangential-flow filtration through a filtration membrane having a pore size that separates the polypeptides or proteins from the mixture, while maintaining flux at a level ranging from about 5 to 100% of transition point flux in the pressure-dependent region of the flux versus TMP curve, whereby the proteins or polypeptides are selectively separated from the mixture, and wherein transmembrane pressure decreases or remains approximately constant as the filtration progresses.
Claim 14
Dependent Claim
112 words
A process for separating at least two proteins or polypeptides from each other that are contained in a mixture, wherein the proteins or polypeptides differ from each other in molecular weight by less than ten fold, which process comprises filtering the mixture by tangential-flow filtration through a filtration membrane having a pore size that separates the polypeptides or proteins from the mixture, while maintaining flux at a level ranging from about 5 to 100% of transition point flux in the pressure-dependent region of the flux versus TMP curve, whereby the proteins or polypeptides are selectively separated from the mixture, and wherein transmembrane pressure decreases or remains approximately constant as the filtration progresses.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-02-12 | 1999-08-14 | 2000-02-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-02-12 | 2003-08-14 | 2004-02-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-02-12 | 2007-08-14 | 2008-02-13 | $7700.00 | Window Closed / Lapsed |