Analyte detection with multilayered bioelectronic conductivity sensors

Claim 1

A method for detecting an analyte in a sample with a bioelectronic sensor, said sensor comprising: an electrically inert substrate; an electrode array comprising a plurality of interdigitating parallel electrodes supported by said substrate, defining two sets of parallel electrodes, each of said sets having a common lead; a surfactant layer comprising crystalline domains of an electrically conducting surfactant polymer in electrical contact with said electrodes, said polymer formed by the polymerization of alkadiynes of the formula: C.sub.x (C.tbd.C).sub.n C.sub.y L M wherein: C intends carbon; x and y are at least 1 and the sum of x and y is in the range of 4-32; n is 2; L is a bond or linking group; and M is a specific binding member which specifically binds to said analyte; with one terminus of each of said alkadiynes proximal to said substrate and the other terminus comprising said specific binding member, said polymerization occurring at the C.tbd.C groups of said alkadiynes, wherein specific binding of said analyte to said specific binding member causes a change in the conductivity of said electrically conducting surfactant polymer; insulating means proximal to said electrodes and leads for insulating said electrodes and leads from external contact with moisture; and means for connecting said leads to an external circuit; said method consisting of: contacting said sample with said bioelectronic sensor; detecting the change in the conductivity of said electrically conducting polymer; and relating said change to the presence of said analyte in said sample.

Claim 2

A method for detecting an analyte in a sample with a bioelectronic sensor, said sensor comprising: an electrically inert substrate; an electrode array comprising a plurality of interdigitating parallel electrodes supported by said substrate, defining two sets of parallel electrodes, each of said sets having a common lead; a surfactant layer comprising crystalline domains of an electrically conducting surfactant polymer in electrical contact with said electrodes, said polymer formed by the polymerization of alkadiynes of the formula: C.sub.x (C.tbd.C).sub.n C.sub.y L M wherein: C intends carbon; x and y are at least 1 and the sum of x and y is in the range of 4-32; n is 2; L is a bond or linking group; and M is a specific binding member which specifically binds to said analyte; with one terminus of each of said alkadiynes proximal to said substrate and the other terminus comprising said specific binding member, said polymerization occurring at the C.tbd.C groups of said alkadiynes, wherein specific binding of said analyte to said specific binding member causes a change in the conductivity of said electrically conducting surfactant polymer; insulating means proximal to said electrodes and leads for insulating said electrodes and leads from external contact with moisture; and means for connecting said leads to an external circuit; said method consisting of: contacting said sample with said bioelectronic sensor; detecting the change in the conductivity of said electrically conducting polymer; and relating said change to the presence of said analyte in said sample.

Claim 5

A method for detecting an analyte in a sample with a bioelectronic sensor, said sensor comprising: an electrically inert substrate; an electrode array comprising a plurality of interdigitating parallel electrodes supported by said substrate, defining two sets of parallel electrodes, each of said sets having a common lead; a surfactant layer comprising crystalline domains of an electrically conducting surfactant polymer in electrical contact with said electrodes, said polymer formed by the polymerization of alkadiynes of the formula: C.sub.x (C.tbd.C).sub.n C.sub.y L M wherein: C intends carbon; x and y are at least 1 and the sum of x and y is in the range of 4-32; n is 2; L is a bond or linking group; and M is a first specific binding member which specifically binds to said analyte or an analyte competitor; with one terminus of each of said alkadiynes proximal to said substrate and the other terminus comprising said specific binding member, said polymerization occurring at the C.tbd.C groups or said alkadiynes, wherein specific binding of said analyte or said analyte competitor to said specific binding member causes a change in the conductivity of said electrically conducting surfactant polymer; insulating means proximal to said electrodes and leads for insulating said electrodes and leads from external contact with moisture; and means for connecting said leads to an external circuit; said method consisting of: combining said sample with said analyte competitor in an assay medium; contacting said assay medium with said bioelectronic sensor; detecting the change in the conductivity of said electrically conducting polymer; and relating said change to the presence of said analyte in said sample.

Claim 6

A method for detecting an analyte in a sample with a bioelectronic sensor, said sensor comprising: an electrically inert substrate; an electrode array comprising a plurality of interdigitating parallel electrodes supported by said substrate, defining two sets of parallel electrodes, each of said sets having a common lead; a surfactant layer comprising crystalline domains of an electrically conducting surfactant polymer in electrical contact with said electrodes, said polymer formed by the polymerization of alkadiynes of the formula: C.sub.x (C.tbd.C).sub.n C.sub.y L M wherein: C intends carbon; x and y are at least 1 and the sum of x and y is in the range of 4-32; n is 2; L is a bond or linking group; and M is a first specific binding member which specifically binds to said analyte or an analyte competitor; with one terminus of each of said alkadiynes proximal to said substrate and the other terminus comprising said specific binding member, said polymerization occurring at the C.tbd.C groups or said alkadiynes, wherein specific binding of said analyte or said analyte competitor to said specific binding member causes a change in the conductivity of said electrically conducting surfactant polymer; insulating means proximal to said electrodes and leads for insulating said electrodes and leads from external contact with moisture; and means for connecting said leads to an external circuit; said method consisting of: combining said sample with said analyte competitor in an assay medium; contacting said assay medium with said bioelectronic sensor; detecting the change in the conductivity of said electrically conducting polymer; and relating said change to the presence of said analyte in said sample.

Claim 7

A method for detecting an analyte in a sample with a bioelectronic sensor, said sensor comprising: an electrically inert substrate; an electrode array comprising a plurality of interdigitating parallel electrodes supported by said substrate, defining two sets of parallel electrodes, each of said sets having a common lead; a surfactant layer comprising crystalline domains of an electrically conducting surfactant polymer in electrical contact with said electrodes, said polymer formed by the polymerization of alkadiynes of the formula: C.sub.x (C.tbd.C).sub.n C.sub.y L M wherein: C intends carbon; x and y are at least 1 and the sum of x and y is in the range of 4-32; n is 2; L is a bond or linking group; and M is a first specific binding member which binds to a conjugate, wherein said conjugate comprises a receptor which specifically binds said analyte and a second specific binding member which specifically binds to said first specific binding member; with one terminus of each of said alkadiynes proximal to said substrate and the other terminus comprising said specific binding member, said polymerization occurring at the C.tbd.C groups of said alkadiynes, wherein specific binding of said analyte to said conjugate causes a change in the conductivity of said electrically conducting surfactant polymer; insulating means proximal to said electrodes and leads for insulating said electrodes and leads from external contact with moisture; and means for connecting said leads to an external circuit; said method consisting of: combining said sample with said conjugate in an assay medium; contacting said assay medium with said bioelectronic sensor; detecting the change in the conductivity of said electrically conducting polymer; and relating said change to the presence of said analyte in said sample.

Claim 8

A method for detecting an analyte in a sample with a bioelectronic sensor, said sensor comprising: an electrically inert substrate; an electrode array comprising a plurality of interdigitating parallel electrodes supported by said substrate, defining two sets of parallel electrodes, each of said sets having a common lead; a surfactant layer comprising crystalline domains of an electrically conducting surfactant polymer in electrical contact with said electrodes, said polymer formed by the polymerization of alkadiynes of the formula: C.sub.x (C.tbd.C).sub.n C.sub.y L M wherein: C intends carbon; x and y are at least 1 and the sum of x and y is in the range of 4-32; n is 2; L is a bond or linking group; and M is a first specific binding member which binds to a conjugate, wherein said conjugate comprises a receptor which specifically binds said analyte and a second specific binding member which specifically binds to said first specific binding member; with one terminus of each of said alkadiynes proximal to said substrate and the other terminus comprising said specific binding member, said polymerization occurring at the C.tbd.C groups of said alkadiynes, wherein specific binding of said analyte to said conjugate causes a change in the conductivity of said electrically conducting surfactant polymer; insulating means proximal to said electrodes and leads for insulating said electrodes and leads from external contact with moisture; and means for connecting said leads to an external circuit; said method consisting of: combining said sample with said conjugate in an assay medium; contacting said assay medium with said bioelectronic sensor; detecting the change in the conductivity of said electrically conducting polymer; and relating said change to the presence of said analyte in said sample.