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US5504002A
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Cryopreserving bovine embryos with a composition comprising 4% ethylen E glycol, 4% propanediol, and bovine albumin having a lipid content of 2.5 ug or more
Assignee: National Federation of Agricultural Cooperative Associations
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Filing: 1994-02-03
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Granted: 1996-04-02
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Expires: 2014-02-03
Abstract
Abstract A method for freezing bovine embryos with a composition which includes 4% ethylene glycol and 4% propanediol and in addition 4% bovine serum albumin containing a high lipid content is disclosed. The mixture is subjected to freezing and used in a method for reproducing cattle by transferring to a cow recipient directly after thawing the preserved embryos. Further, a high lipid content is disclosed as 2.5 ug or more of lipid/mg of bovine serum albumin. The bovine embryos are obtained by in vivo fertilization or by in vitro fertilization for use in the disclosed methods.
Inventors
- 👤 Yoshito Aoyagi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 20:13
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/125
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
51 words
A method for freezing bovine embryos which comprises mixing a bovine embryo with a composition comprising a cryoprotectant comprising 4% ethylene glycol and 4% propanediol, said composition further comprising 4% bovine serum albumin which contains at least 2.5 ug of lipid/mg of bovine serum albumin, and then freezing the resulting mixture.
Claim 2
Independent Claim
51 words
A method for freezing bovine embryos which comprises mixing a bovine embryo with a composition comprising a cryoprotectant comprising 4% ethylene glycol and 4% propanediol, said composition further comprising 4% bovine serum albumin which contains at least 2.5 ug of lipid/mg of bovine serum albumin, and then freezing the resulting mixture.
Claim 3
Independent Claim
56 words
A method for reproducing cattle which comprises cryopreserving bovine embryos in a composition comprising a cryoprotectant comprising 4% ethylene glycol and 4% propanediol, said composition further comprising 4% bovine serum albumin which contains at least 2.5 ug of lipid/mg of bovine serum albumin, thawing the preserved embryos, and then transferring the thawed embryos directly to recipients.
Claim 4
Independent Claim
56 words
A method for reproducing cattle which comprises cryopreserving bovine embryos in a composition comprising a cryoprotectant comprising 4% ethylene glycol and 4% propanediol, said composition further comprising 4% bovine serum albumin which contains at least 2.5 ug of lipid/mg of bovine serum albumin, thawing the preserved embryos, and then transferring the thawed embryos directly to recipients.
Claim 5
Dependent Claim
22 words
A method according to claim 1, wherein the bovine embryos are those obtained by in vivo fertilization or by in vitro fertilization.
Claim 6
Dependent Claim
22 words
A method according to claim 2, wherein the bovine embryos are those obtained by in vivo fertilization or by in vitro fertilization.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-04-02 | 1999-10-02 | 2000-04-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-04-02 | 2003-10-02 | 2004-04-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-04-02 | 2007-10-02 | 2008-04-02 | $7700.00 | Window Closed / Lapsed |