US5538893A
Expired / Lapsed
US
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Reagent for analyzing leukocytes and a method for classifying leukocytes
Assignee: Sysmex Corp
•
Filing: 1995-04-20
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Granted: 1996-07-23
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Expires: 2015-04-20
Abstract
Abstract A reagent for analyzing leukocytes having at least one nonionic surfactant having an addition polymerization molar number of polyoxyethylene of 3 to 9, at least one cationic surfactant, and a buffer adjusting a pH value to 2.5 to 4.0, which can determine a cell size of and the morphological features of leukocytes contained in the blood sample.
Inventors
- 👤 Takashi Sakata
- 👤 Yasumasa Akai
- 👤 Kaoru Takarada
- 👤 Chihiro Kouzuki
- 👤 Yoshihiro Hyousa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 17:48
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5094; G01N15/00; G01N15/10; G01N15/14; G01N15/1434; G01N15/1456; G01N15/1459; G01N2015/1006; G01N2015/1402; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/107497; Y10T436/25; Y10T436/25125
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
45 words
A reagent for treating a blood sample for classifying and counting leukocytes comprising: at least one nonionic surfactant having an additional mole number of polyoxyethylene of 3 to 9, at least one cationic surfactant, and a buffer adjusting a pH value to 2.5 to 4.0.
Claim 2
Dependent Claim
45 words
A reagent for treating a blood sample for classifying and counting leukocytes comprising: at least one nonionic surfactant having an additional mole number of polyoxyethylene of 3 to 9, at least one cationic surfactant, and a buffer adjusting a pH value to 2.5 to 4.0.
Claim 3
Dependent Claim
21 words
A reagent according to claim 1 further comprising an alkali metal halide such as sodium chloride, potassium chloride or lithium chloride.
Claim 4
Dependent Claim
55 words
A reagent according to claim 1 or claim 2, in which the at least one nonionic surfactant has the formula: R.sub.1 --R.sub.2 --(CH.sub.2 CH.sub.2 O).sub.n --H wherein R 1 is a C 8 -C 18 alkyl or alkenyl group, R 2 is --O-- or, ##STR2## and n is a real number of 3 to 9.
Claim 5
Dependent Claim
41 words
A reagent according to claim 3, in which the at least one nonionic surfactant is polyoxyethylene (4.2) dodecyl ether, polyoxyethylene (9) dodecyl ether, polyoxyethylene (5) dodecyl ether, polyoxyethylene (5.5) cethyl ether, polyoxyethylene (? ) cethyl ether or polyoxyethylene (7) oleyl ether.
Claim 6
Dependent Claim
87 words
A reagent according to claim 1 or claim 2, in which the at least one cationic surfactant is a quaternary ammonium salt having the formula: ##STR3## wherein R 1 is a C 10 -C 18 alkyl or alkenyl group, each of R 2 , R 3 and R 4 is a C 1 -C 3 alkyl or alkenyl group and X is a halogen group; or a pyridinium salt having the formula: R.sub.1 --N.sup.+ C.sub.5 H.sub.5 X.sup.- wherein R 1 and X - are defined as above.
Claim 7
Dependent Claim
35 words
A reagent according to claim 5, in which the at least one cationic surfactant is dodecyltrimethylammonium bromide, lauryltrimethyl ammonium chloride, myristyltrimethyl ammonium bromide, cetyltrimethyl ammonium chloride, stearyltrimethyl ammonium chloride, cetyldimethylethyl ammonium bromide or cetylpyridinium chloride.
Claim 8
Dependent Claim
43 words
A reagent according to claim 1, in which the at least one nonionic surfactant is present at a concentration of 300 mg/l to 20,000 mg/l and the at least one cationic surfactant is present at a concentration of 100 mg/l to 10,000 mg/l.
Claim 9
Independent Claim
70 words
A method for classifying and counting leukocytes comprising: mixing with a blood sample a reagent which comprises at least one nonionic surfactant having an additional mole number of polyoxyethylene of 3 to 9, at least one cationic surfactant, and a buffer adjusting a pH value to 2.5 to 4.0, classifying and counting leukocytes by measuring cell size and at least one morphological feature of cells contained in the blood sample.
Claim 10
Independent Claim
70 words
A method for classifying and counting leukocytes comprising: mixing with a blood sample a reagent which comprises at least one nonionic surfactant having an additional mole number of polyoxyethylene of 3 to 9, at least one cationic surfactant, and a buffer adjusting a pH value to 2.5 to 4.0, classifying and counting leukocytes by measuring cell size and at least one morphological feature of cells contained in the blood sample.
Claim 11
Dependent Claim
38 words
A method according to claim 8, in which the size of a cell is determined by measuring the forward low angle scattered light using a flow cytometer, or by measuring cell volume by detecting an electric impedance signal.
Claim 12
Dependent Claim
42 words
A method according to claim 8, in which the at least one morphological feature of cells is determined by using a flow cytometer and measuring the light side scattered from a cell or the light forward high angle scattered from a cell.
References Cited (Backward Citations)
US4286963A
Cited
US4528274A
Cited
US4485175A
Cited
JPS61188896A
Cited
US4745071A
Cited
US4933293A
Cited
US5039613A
Cited
US5389549A
Cited
EP0325710A2
Cited
US5116539A
Cited
JPH0320667A
Cited
EP0424871A1
Cited
US5250437A
Cited
US5232857A
Cited
US5196346A
Cited
JPH04230854A
Cited
US5242832A
Cited
US5413938A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-07-23 | 2000-01-22 | 2000-07-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-07-23 | 2004-01-22 | 2004-07-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-07-23 | 2008-01-22 | 2008-07-23 | $7700.00 | Window Closed / Lapsed |