US5948632A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and reagent for measuring chlorine and calcium ions using a maltose derivative
Assignee: Oriental Yeast Co Ltd
•
Filing: 1996-11-27
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Granted: 1999-09-07
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Expires: 2016-11-27
Abstract
Abstract Chlorine and calcium ions are measured in a biological sample using a maltose derivative having a chromogenic group at the reducing end. These ions are accurately measured in biological samples such as blood and urine without influence of amylase that may be present in such samples.
Inventors
- 👤 Yukako Moritani
- 👤 Setsuko Takahata
- 👤 Masashi Nakagawa
- 👤 Seiichi Kohda
- 👤 Tuyosi Fujita
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 21:08
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/40; C12Q2334/00; C12Q2334/10; Y; Y10; Y10S; Y10S435/00; Y10S435/962
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
97 words
A method of measuring concentration of chlorine ions or calcium ions in a sample, said method comprising the steps of: (a) contacting in the presence of amylase a sample containing chlorine ions or calcium ions with a reagent of the formula: ##STR3## wherein X is a phenyl or substituted phenyl chromophore, whereby chlorine or calcium ions hydrolyze said reagent and release said chromophore; (b) determining the quantity of chromophore released in step (a); and (c) correlating the quantity of released chromophore measured in step (b) to the concentration of chlorine ions or calcium ions in the sample.
Claim 2
Independent Claim
97 words
A method of measuring concentration of chlorine ions or calcium ions in a sample, said method comprising the steps of: (a) contacting in the presence of amylase a sample containing chlorine ions or calcium ions with a reagent of the formula: ##STR3## wherein X is a phenyl or substituted phenyl chromophore, whereby chlorine or calcium ions hydrolyze said reagent and release said chromophore; (b) determining the quantity of chromophore released in step (a); and (c) correlating the quantity of released chromophore measured in step (b) to the concentration of chlorine ions or calcium ions in the sample.
Claim 3
Dependent Claim
14 words
The method of claim 1 in which the chromophore is 2-chloro-4-nitrophenyl, paranitrophenyl or 6-dichloro-4-nitrophenyl.
Claim 4
Dependent Claim
41 words
A reagent for measuring concentration of chlorine ions or calcium ions comprising a maltose derivative of the formula: ##STR4## wherein X is a phenyl or substituted phenyl chromophore in which chlorine ions or calcium ions promote hydrolysis of said maltose derivative.
Claim 5
Dependent Claim
41 words
A reagent for measuring concentration of chlorine ions or calcium ions comprising a maltose derivative of the formula: ##STR4## wherein X is a phenyl or substituted phenyl chromophore in which chlorine ions or calcium ions promote hydrolysis of said maltose derivative.
Claim 6
Dependent Claim
14 words
The reagent of claim 3 in which the chromophore is 2-chloro-4-nitrophenyl, paranitrophenyl, or 6-dichloro-4-nitrophenyl.
References Cited (Backward Citations)
EP0085348A1
Cited
JPS6236199A
Cited
EP0486470A1
Cited
EP0275398A2
Cited
JPS63126497A
Cited
US5229270A
Cited
US5384246A
Cited
JPS642598A
Cited
JPH01231896A
Cited
EP0510620A1
Cited
US5618684A
Cited
JPH06113894A
Cited
WO1995004831A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-09-06 | 2003-03-08 | 2003-09-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-09-06 | 2007-03-08 | 2007-09-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-09-06 | 2011-03-08 | 2011-09-07 | $7700.00 | Window Closed / Lapsed |