US6248596B1
Expired / Lapsed
US
⏱️ 0.0 years remaining
Liposome-enhanced immunoassay and test device
Assignee: Cornell Research Foundation Inc
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Filing: 1998-03-03
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Granted: 2001-06-19
•
Expires: 2018-03-03
Abstract
Abstract A test device for detecting or determining an analyte in a test solution includes an absorbent material having separate contact, competitive binding, and measurement portions. The contact portion is positioned for contact with and uptake of the test solution. The competitive binding portion has a binding material for the analyte non-diffusively bound thereto. The measurement portion has a receptor for the analyte and marker-encapsulating liposomes non-diffusively bound thereto. In a method for using the test device, a solution containing the analyte and the analyte-liposome conjugate is allowed to traverse the absorbent material from the contact portion through the competitive binding portion and on through the measurement portion of the absorbent material. The amount of marker in the measurement portion of the absorbent material, following traversal by the test solution, is then determined as a measure of the analyte in the sample. Liposomes encapsulating an electroactive marker are used in conjunction with a test device as described above but which includes an electrochemical measurement portion in place of the measurement portion described above. Test devices and methods employing electrochemical detection or quantification of an electroactive marker corresponding to the amount of analyte in a sample may be either amperometric or potentiometric.
Inventors
- 👤 Richard Allen Durst
- 👤 Stuart Graham Reeves
- 👤 Sui Ti Atienza Siebert
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 00:24
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54366; G01N33/54373; G01N33/5438; G01N33/54386; G01N33/54387; G01N33/54388; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10S435/97; Y10S436/00; Y10S436/805; Y10S436/806; Y10S436/807; Y10S436/81; Y10S436/815; Y10S436/829
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
227 words
A test device for detecting or determining an analyte in a test solution, said test device comprising: an absorbent material, comprising: a contact portion proximate to a first end of said absorbent material for contact with and uptake of said test solution; an electrochemical measurement portion at a location on said absorbent material which is positioned away from the first end; and a competitive binding portion positioned between and segregated from said contact portion and said measurement portion on said absorbent material and having a binding material for the analyte non-diffusively bound to said competitive binding portion; said electrochemical measurement portion comprising a working electrode portion and a counter electrode portion, and further comprising a reference electrode portion positioned between and segregated from said working electrode portion and said counter electrode portion on said absorbent material, wherein said working electrode portion is positioned between said competitive binding portion and said reference electrode portion on said absorbent material, said working, reference, and counter electrode portions are electrically connected with one another, and wherein either said absorbent material further comprises a liposome lysing portion positioned between said competitive binding portion and said working electrode portion, wherein said liposome lysing portion is segregated from said competitive binding portion and has a liposome lysing agent non-diffusively bound thereto, or said working electrode portion has a liposome lysing agent non-diffusively bound thereto.
Claim 2
Dependent Claim
227 words
A test device for detecting or determining an analyte in a test solution, said test device comprising: an absorbent material, comprising: a contact portion proximate to a first end of said absorbent material for contact with and uptake of said test solution; an electrochemical measurement portion at a location on said absorbent material which is positioned away from the first end; and a competitive binding portion positioned between and segregated from said contact portion and said measurement portion on said absorbent material and having a binding material for the analyte non-diffusively bound to said competitive binding portion; said electrochemical measurement portion comprising a working electrode portion and a counter electrode portion, and further comprising a reference electrode portion positioned between and segregated from said working electrode portion and said counter electrode portion on said absorbent material, wherein said working electrode portion is positioned between said competitive binding portion and said reference electrode portion on said absorbent material, said working, reference, and counter electrode portions are electrically connected with one another, and wherein either said absorbent material further comprises a liposome lysing portion positioned between said competitive binding portion and said working electrode portion, wherein said liposome lysing portion is segregated from said competitive binding portion and has a liposome lysing agent non-diffusively bound thereto, or said working electrode portion has a liposome lysing agent non-diffusively bound thereto.
Claim 3
Dependent Claim
21 words
A test device according to claim 1 , said working electrode portion further having a marker accumulating agent non-diffusively bound thereto.
Claim 4
Dependent Claim
208 words
A test device for detecting or determining an analyte in a test solution, said test device comprising: an absorbent material, comprising: a contact portion proximate to a first end of said absorbent material for contact with and uptake of said test solution; an electrochemical measurement portion at a location on said absorbent material which is positioned away from the first end; and a competitive binding portion positioned between and segregated from said contact portion and said measurement portion on said absorbent material and having a binding material for the analyte non-diffusively bound to said competitive binding portion; said electrochemical measurement portion comprising an indicator electrode portion and a reference electrode portion, wherein said indicator electrode portion and said reference electrode portion are electrically connected with one another, and said indicator electrode portion is positioned between and segregated from said competitive binding portion and said reference electrode portion on said absorbent material, and wherein either said absorbent material further comprises a liposome lysing portion positioned between said competitive binding portion and said indicator electrode portion, wherein said liposome lysing portion is segregated from said competitive binding portion and has a liposome lysing agent non-diffusively bound thereto, or said indicator electrode portion has a liposome lysing agent non-diffusively bound thereto.
Claim 5
Dependent Claim
208 words
A test device for detecting or determining an analyte in a test solution, said test device comprising: an absorbent material, comprising: a contact portion proximate to a first end of said absorbent material for contact with and uptake of said test solution; an electrochemical measurement portion at a location on said absorbent material which is positioned away from the first end; and a competitive binding portion positioned between and segregated from said contact portion and said measurement portion on said absorbent material and having a binding material for the analyte non-diffusively bound to said competitive binding portion; said electrochemical measurement portion comprising an indicator electrode portion and a reference electrode portion, wherein said indicator electrode portion and said reference electrode portion are electrically connected with one another, and said indicator electrode portion is positioned between and segregated from said competitive binding portion and said reference electrode portion on said absorbent material, and wherein either said absorbent material further comprises a liposome lysing portion positioned between said competitive binding portion and said indicator electrode portion, wherein said liposome lysing portion is segregated from said competitive binding portion and has a liposome lysing agent non-diffusively bound thereto, or said indicator electrode portion has a liposome lysing agent non-diffusively bound thereto.
Claim 6
Dependent Claim
200 words
A test device for detecting or determining an analyte in a test solution, said test device comprising: an absorbent material, comprising: a contact portion proximate to a first end of said absorbent material for contact with and uptake of said test solution; an electrochemical measurement portion at a location on said absorbent material which is positioned away from the first end; and a competitive binding portion positioned between and segregated from said contact portion and said measurement portion on said absorbent material and having a binding material for the analyte non-diffusively bound to said competitive binding portion; said electrochemical measurement portion comprising a working electrode portion, a reference electrode portion, and a counter electrode portion, wherein each of said electrode portions is segregated from one another on said absorbent material, said working, reference, and counter electrode portions are electrically connected with one another, and wherein either said absorbent material further comprises a liposome lysing portion positioned between said competitive binding portion and said working electrode portion, wherein said liposome lysing portion is segregated from said competitive binding portion and has a liposome lysing agent non-diffusively bound thereto, or said working electrode portion has a liposome lysing agent non-diffusively bound thereto.
Claim 7
Dependent Claim
200 words
A test device for detecting or determining an analyte in a test solution, said test device comprising: an absorbent material, comprising: a contact portion proximate to a first end of said absorbent material for contact with and uptake of said test solution; an electrochemical measurement portion at a location on said absorbent material which is positioned away from the first end; and a competitive binding portion positioned between and segregated from said contact portion and said measurement portion on said absorbent material and having a binding material for the analyte non-diffusively bound to said competitive binding portion; said electrochemical measurement portion comprising a working electrode portion, a reference electrode portion, and a counter electrode portion, wherein each of said electrode portions is segregated from one another on said absorbent material, said working, reference, and counter electrode portions are electrically connected with one another, and wherein either said absorbent material further comprises a liposome lysing portion positioned between said competitive binding portion and said working electrode portion, wherein said liposome lysing portion is segregated from said competitive binding portion and has a liposome lysing agent non-diffusively bound thereto, or said working electrode portion has a liposome lysing agent non-diffusively bound thereto.
Claim 8
Dependent Claim
21 words
A test device according to claim 4 , said working electrode portion further having a marker accumulating agent non-diffusively bound thereto.
Claim 9
Dependent Claim
194 words
A test device for detecting or determining an analyte in a test solution, said test device comprising: an absorbent material, comprising: a contact portion proximate to a first end of said absorbent material for contact with and uptake of said test solution; an electrochemical measurement portion at a location on said absorbent material which is positioned away from the first end; and a competitive binding portion positioned between and segregated from said contact portion and said measurement portion on said absorbent material and having a binding material for the analyte non-diffusively bound to said competitive binding portion; said electrochemical measurement portion comprising an indicator electrode portion and a reference electrode portion, wherein said indicator electrode portion and said reference electrode portion are segregated from one another on said absorbent material and are electrically connected with one another, and wherein either said absorbent material further comprises a liposome lysing portion positioned between said competitive binding portion and said indicator electrode portion, wherein said liposome lysing portion is segregated from said competitive binding portion and has a liposome lysing agent non-diffusively bound thereto, or said indicator electrode portion has a liposome lysing agent non-diffusively bound thereto.
Claim 10
Dependent Claim
194 words
A test device for detecting or determining an analyte in a test solution, said test device comprising: an absorbent material, comprising: a contact portion proximate to a first end of said absorbent material for contact with and uptake of said test solution; an electrochemical measurement portion at a location on said absorbent material which is positioned away from the first end; and a competitive binding portion positioned between and segregated from said contact portion and said measurement portion on said absorbent material and having a binding material for the analyte non-diffusively bound to said competitive binding portion; said electrochemical measurement portion comprising an indicator electrode portion and a reference electrode portion, wherein said indicator electrode portion and said reference electrode portion are segregated from one another on said absorbent material and are electrically connected with one another, and wherein either said absorbent material further comprises a liposome lysing portion positioned between said competitive binding portion and said indicator electrode portion, wherein said liposome lysing portion is segregated from said competitive binding portion and has a liposome lysing agent non-diffusively bound thereto, or said indicator electrode portion has a liposome lysing agent non-diffusively bound thereto.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2004-06-18 | 2004-12-18 | 2005-06-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2008-06-18 | 2008-12-18 | 2009-06-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2012-06-18 | 2012-12-18 | 2013-06-19 | $7700.00 | Window Closed / Lapsed |