US6436256B1
Expired / Lapsed
US
⏱️ 0.0 years remaining
Electrodes for the measurement of analytes in small sample volumes
Assignee: Cambridge Sensors Ltd
•
Filing: 1998-06-03
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Granted: 2002-08-20
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Expires: 2018-06-03
Abstract
Abstract A device, for use in the electrochemical analysis of an analyte in a small volume liquid sample, having a non-conducting substrate ( 1 ); a conductive layer, deposited on the substrate, in two parts ( 2 a, 2 b ), defining a non-conducting gap ( 8 ) therebetween; an analyte-specific reagent ( 5 ) coated on the conductive layer, on one side of the gap; a reference electrode ( 3 ) on the conductive layer, on the other side of the gap; a spacer layer ( 4 ) deposited over the conductive layer; a monofilament mesh ( 6 ) coated with a surfactant or chaotropic agent, the mesh being laid over the reagent, the reference electrode and the spacer layer; and a second non-conductive layer ( 7 ) adhered to the mesh layer, but not coextensive therewith, thereby providing a sample application area ( 9 ) on the mesh.
Inventors
- 👤 Stephen Charles Williams
- 👤 Bernadette Yon-Hin
- 👤 Neil Blair
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 18:21
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/28; G01N27/30; G01N27/327; G01N27/3271; G01N27/3272
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
152 words
A device for use in the electrochemical analysis of an analyte in a liquid sample, which comprises: a non-conducting substrate; a discontinuous conductive layer deposited on adjacent first and second portions, respectively, of the non-conducting substrate and defining a non-conducting gap between the first and second portions; an analyte-specific reagent coated on the conductive layer on the first portion; a reference electrode on the the conductive layer on the second portion; a spacer layer deposited over the conductive layer; a monofilament mesh coated with a surfactant or chaotropic agent, the mesh being laid over the analyte-specific reagent, the reference electrode and the spacer layer; and a second non-conductive layer, adhered to and covering the mesh layer, said second non-conducting layer having an exterior edge such that the second non-conducting layer is not co-extensive with the mesh layer, thereby providing an exposed portion of the mesh at one exterior edge of the mesh.
Claim 2
Independent Claim
152 words
A device for use in the electrochemical analysis of an analyte in a liquid sample, which comprises: a non-conducting substrate; a discontinuous conductive layer deposited on adjacent first and second portions, respectively, of the non-conducting substrate and defining a non-conducting gap between the first and second portions; an analyte-specific reagent coated on the conductive layer on the first portion; a reference electrode on the the conductive layer on the second portion; a spacer layer deposited over the conductive layer; a monofilament mesh coated with a surfactant or chaotropic agent, the mesh being laid over the analyte-specific reagent, the reference electrode and the spacer layer; and a second non-conductive layer, adhered to and covering the mesh layer, said second non-conducting layer having an exterior edge such that the second non-conducting layer is not co-extensive with the mesh layer, thereby providing an exposed portion of the mesh at one exterior edge of the mesh.
Claim 3
Dependent Claim
21 words
A device according to claim 1 , wherein the reagent does not contain filler having both hydrophobic and hydrophilic surface regions.
Claim 4
Dependent Claim
18 words
A device according to claim 2 , wherein the analyte is glucose and the reagent is glucose dehydrogenase.
Claim 5
Dependent Claim
18 words
A device according to claim 2 , wherein the mesh is additionally coated with a cell lytic agent.
Claim 6
Dependent Claim
64 words
A device according to claim 2 , wherein the first conductive layer comprises graphite particles, carbon particles and a polymer binder, wherein the graphite particles have an average particle size of 1-20 μm and a surface area of 1-50 m 2 /g, and the carbon particles have an average size of 5-70 nm and a surface area of less than 150 m 2 /g.
Claim 7
Dependent Claim
39 words
A method for the electrochemical analysis of an analyte in a liquid sample, which comprises applying the sample to the application area on a device according to claim 2 , and quantifying the analyte by reaction with the reagent.
Claim 8
Dependent Claim
18 words
A device according to claim 1 , wherein the analyte is glucose and the reagent is glucose dehydrogenase.
Claim 9
Dependent Claim
18 words
A device according to claim 1 , wherein the mesh is additionally coated with a cell lytic agent.
Claim 10
Dependent Claim
64 words
A device according to claim 1 , wherein the first conductive layer comprises graphite particles, carbon particles and a polymer binder, wherein the graphite particles have an average particle size of 1-20 μm and a surface area of 1-50 m 2 /g, and the carbon particles have an average size of 5-70 nm and a surface area of less than 150 m 2 /g.
Claim 11
Dependent Claim
39 words
A method for the electrochemical analysis of an analyte in a liquid sample, which comprises applying the sample to the application area on a device according to claim 1 , and quantifying the analyte by reaction with the reagent.
Claim 12
Independent Claim
156 words
A device for use in the electrochemical analysis of an analyte in a liquid sample, which comprises: a non-conducting substrate; a first and a second conductive layer deposited on first and second portions, respectively, of the non-conducting substrate and defining a non-conducting gap between the first and second conductive layers; an analyte-specific reagent coated on the first conductive layer; a reference electrode on the second conductive layer; a spacer layer deposited over a portion of both the first and second conductive layers; a monofilament mesh coated with a surfactant or chaotropic agent, the mesh being laid over the analyte-specific reagent, the reference electrode and the spacer layer; and a second non-conductive layer, adhered to and covering the mesh layer, said second non-conducting layer having an exterior edge such that the second non-conducting layer is not co-extensive with the mesh layer, thereby providing an exposed edge portion of the mesh at one exterior edge of the mesh.
Claim 13
Independent Claim
156 words
A device for use in the electrochemical analysis of an analyte in a liquid sample, which comprises: a non-conducting substrate; a first and a second conductive layer deposited on first and second portions, respectively, of the non-conducting substrate and defining a non-conducting gap between the first and second conductive layers; an analyte-specific reagent coated on the first conductive layer; a reference electrode on the second conductive layer; a spacer layer deposited over a portion of both the first and second conductive layers; a monofilament mesh coated with a surfactant or chaotropic agent, the mesh being laid over the analyte-specific reagent, the reference electrode and the spacer layer; and a second non-conductive layer, adhered to and covering the mesh layer, said second non-conducting layer having an exterior edge such that the second non-conducting layer is not co-extensive with the mesh layer, thereby providing an exposed edge portion of the mesh at one exterior edge of the mesh.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2005-08-19 | 2006-02-18 | 2006-08-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2009-08-19 | 2010-02-18 | 2010-08-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2013-08-19 | 2014-02-18 | 2014-08-20 | $7700.00 | Window Closed / Lapsed |