Patent EP0620437A1 successfully refreshed from Google Patents.
EP0620437A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Multilayer test strip
Assignee: DIAGNOSTIC SOLUTIONS Inc
•
Filing: 1994-04-14
•
Granted: —
•
Expires: 2014-04-14
Abstract
Abstract A multilayer test strip includes a first layer having an indicator reagent within a porous substrate, a second layer, overlying the first layer, including a reactive reagent, and a third layer, overlying the second layer, including a spreading agent that promotes the spreading of a liquid upon the third layer. Alternatively, the spreading agent may be mixed into the second layer. An inert backing with an aperture therethrough is positioned over these layers, with the substrate remote from the cover layer.
Inventors
- 👤 Gary Krantz
- 👤 Shuenn-Tzong Chen
- 👤 Adam Zipp
- 👤 Joanne Zeng
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-28 20:51
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
68 words
A multilayer test strip, comprising: a first layer comprising an indicator reagent impregnated into a porous substrate; a second layer, overlying the first layer, comprising a reactive reagent; a third layer, overlying the second layer, comprising a spreading agent that promotes the spreading of a liquid upon the third layer; and an inert backing overlying the third layer, the backing having an aperture therethrough to the third layer.
Claim 2
Dependent Claim
14 words
The test strip of claim 1, wherein the porous substrate is a nylon membrane.
Claim 3
Dependent Claim
11 words
The test strip of claim 1, wherein the indicator is DMAB.
Claim 4
Dependent Claim
21 words
The test strip of claim 1, wherein the reactive reagent is selected from the group consisting of glucose oxidase and peroxidase.
Claim 5
Dependent Claim
22 words
The test strip of claim 1, wherein the spreading agent is selected from the group consisting of cellulose acetate and diatomaceous earth.
Claim 6
Dependent Claim
59 words
A multilayer test strip, comprising: a first layer comprising an indicator reagent impregnated into a porous substrate; a second layer, overlying the first layer, comprising a reactive reagent and a substance promoting the spreading of a liquid upon the second layer; and an inert backing overlying the second layer, the backing having an aperture therethrough to the second layer.
Claim 7
Dependent Claim
14 words
The test strip of claim 6, wherein the porous substrate is a nylon membrane.
Claim 8
Dependent Claim
11 words
The test strip of claim 6, wherein the indicator is DMAB.
Claim 9
Dependent Claim
21 words
The test strip of claim 6, wherein the reactive reagent is selected from the group consisting of glucose oxidase and peroxidase.
Claim 10
Dependent Claim
22 words
The test strip of claim 6, wherein the spreading agent is selected from the group consisting of cellulose acetate and diatomaceous earth.
Claim 11
Dependent Claim
98 words
A multilayer test strip, comprising: a first layer comprising an indicator reagent impregnated into a porous substrate, wherein the indicator reagent is DMAB; a second layer, overlying the first layer, comprising a reactive reagent that reacts with glucose in human blood, the reactive reagent being selected from the group consisting of glucose oxidase and peroxidase, and a third layer, overlying the second layer, comprising a spreading agent that promotes the spreading of a droplet of blood upon the third layer; and an inert backing overlying the third layer, the backing having an aperture therethrough to the third layer.
Claim 12
Dependent Claim
90 words
A multilayer test strip, comprising: a first layer comprising an indicator reagent impregnated into a porous substrate, wherein the indicator reagent is DMAB; a second layer, overlying the first layer, comprising a reactive reagent that reacts with glucose in human blood, the reactive reagent being selected from the group consisting of glucose oxidase and peroxidase, and a spreading agent that promotes the spreading of a droplet of blood upon the second layer; and an inert backing overlying the second layer, the backing having an aperture therethrough to the second layer.
Claim 13
Dependent Claim
38 words
A test strip, comprising: a reaction unit having a spreadability enhancing material at a top surface thereof; and a backing having an aperture therein, to which the reaction unit is affixed with the top surface contacting the backing.
Claim 14
Independent Claim
143 words
A method for preparing a test strip, comprising the steps of: providing a porous substrate; providing an indicator solution containing the indicator DMAB, the indicator solution having a sufficiently low viscosity that it is penetrable into the porous substrate; introducing the indicator solution into the porous substrate; drying the indicator solution within the porous substrate; providing a reactive reagent solution containing a reagent selected from the group consisting of glucose oxidase and peroxidase, the solution having a sufficiently high viscosity that it is not penetrable into the porous substrate; applying the reactive reagent solution as a reactive reagent layer to a surface of the porous substrate; drying the reactive reagent within the reactive reagent layer; and attaching the porous substrate to a backing having an aperture therethrough, with the applied layer adjacent to the backing and the porous substrate remote from the backing.
Claim 15
Dependent Claim
26 words
The method of claim 14, wherein the step of providing a reactive reagent solution includes the step of mixing a spreading solution into the reagent solution.
Claim 16
Dependent Claim
49 words
The method of claim 14, including the additional steps, after the step of drying the reactive reagent and before the step of attaching the porous substrate, of providing a spreading solution containing a spreading agent; and applying the spreading solution as a spreading layer to the reactive reagent layer.
References Cited (Backward Citations)
EP0103901A2
Cited
EP0162301A1
Cited
EP0162302A1
Cited
EP0239990A2
Cited
EP0256806A2
Cited
US4935346A
Cited
EP0322882A2
Cited
EP0325398A1
Cited
EP0416588A2
Cited
EP0418169A2
Cited
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.