Patent US4513086A successfully refreshed from Google Patents.
US4513086A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cloning vectors for use in streptomyces and related organisms
Assignee: Eli Lilly and Co
•
Filing: 1982-08-02
•
Granted: 1985-04-23
•
Expires: 2002-08-02
Abstract
Abstract The present invention discloses selectable recombinant DNA cloning vectors for use in Streptomyces and related organisms.
Inventors
- 👤 Jeffrey T. Fayerman
- 👤 Mark A. Richardson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 01:28
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/76
Claims Analysis
Total Claims: 101
Claim 1
Independent Claim
51 words
A recombinant DNA cloning vector comprising: (a) a functional origin of replication-containing restriction fragment of plasmid pEL103, and (b) one or more DNA segments that convey resistance to at least one antibiotic when transformed into a sensitive restrictionless host cell, said host cell being susceptible to transformation, cell division, and culture.
Claim 2
Dependent Claim
51 words
A recombinant DNA cloning vector comprising: (a) a functional origin of replication-containing restriction fragment of plasmid pEL103, and (b) one or more DNA segments that convey resistance to at least one antibiotic when transformed into a sensitive restrictionless host cell, said host cell being susceptible to transformation, cell division, and culture.
Claim 3
Dependent Claim
19 words
The cloning vector of claim 1 wherein the restriction fragment of pEL103 is the 2.8 kb BamHI restriction fragment.
Claim 4
Dependent Claim
19 words
The cloning vector of claim 1 wherein the restriction fragment of pEL103 is the 19.9 kb BamHI restriction fragment.
Claim 5
Dependent Claim
39 words
The cloning vector of claim 1, 2, or 3 wherein the one or more DNA segments that convey antibiotic resistance are selected from the group of segments that convey resistance to either or both of antibiotics thiostrepton and neomycin.
Claim 6
Dependent Claim
15 words
The cloning vector of claim 4 wherein one DNA segment conveys antibiotic resistance to thiostrepton.
Claim 7
Dependent Claim
15 words
The cloning vector of claim 4 wherein one DNA segment conveys antibiotic resistance to neomycin.
Claim 8
Dependent Claim
46 words
The cloning vector of claim 1, 2, or 3 wherein the one or more DNA segments that convey antibiotic resistance are selected from the group consisting of the 1.6 kb BamHI restriction fragment of plasmid pLR2 and the 3.4 kb BamHI restriction fragment of plasmid pLR1.
Claim 9
Dependent Claim
20 words
The cloning vector of claim 7 wherein one DNA segment is the 1.6 kb BamHI restriction fragment of plasmid pLR2.
Claim 10
Dependent Claim
20 words
The cloning vector of claim 7 wherein one DNA segment is the 3.4 kb BamHI restriction fragment of plasmid pLR1.
Claim 11
Dependent Claim
32 words
The recombinant DNA cloning vector of claim 1 selected from the group consisting of plasmids pEL107, pEL105, pEL109, pEL110, pEL113, pEL114, pEL115, pEL116, pEL108, pEL104, pEL111, pEL112, pEL117, pEL118, pEL119, and pEL120.
Claim 12
Dependent Claim
9 words
The cloning vector of claim 10 which is pEL107.
Claim 13
Dependent Claim
9 words
The cloning vector of claim 10 which is pEL105.
Claim 14
Dependent Claim
9 words
The cloning vector of claim 10 which is pEL109.
Claim 15
Dependent Claim
9 words
The cloning vector of claim 10 which is pEL110.
Claim 16
Dependent Claim
9 words
The cloning vector of claim 10 which is pEL113.
Claim 17
Dependent Claim
9 words
The cloning vector of claim 10 which is pEL114.
Claim 18
Dependent Claim
9 words
The cloning vector of claim 10 which is pEL115.
Claim 19
Dependent Claim
9 words
The cloning vector of claim 10 which is pEL116.
Claim 20
Dependent Claim
9 words
The cloning vector of claim 10 which is pEL108.
Claim 21
Dependent Claim
9 words
The cloning vector of claim 10 which is pEL104.
Claim 22
Dependent Claim
9 words
The cloning vector of claim 10 which is pEL111.
Claim 23
Dependent Claim
9 words
The cloning vector of claim 10 which is pEL112.
Claim 24
Dependent Claim
9 words
The cloning vector of claim 10 which is pEL117.
Claim 25
Dependent Claim
9 words
The cloning vector of claim 10 which is pEL118.
Claim 26
Dependent Claim
9 words
The cloning vector of claim 10 which is pEL119.
Claim 27
Dependent Claim
9 words
The cloning vector of claim 10 which is pEL120.
Claim 28
Dependent Claim
10 words
A functional origin of replication-containing restriction fragment of plasmid pEL103.
Claim 29
Dependent Claim
10 words
A functional origin of replication-containing restriction fragment of plasmid pEL103.
Claim 30
Dependent Claim
9 words
The 2.8 kb BamHI restriction fragment of plasmid pEL103.
Claim 31
Dependent Claim
9 words
The 2.8 kb BamHI restriction fragment of plasmid pEL103.
Claim 32
Dependent Claim
65 words
A recombinant DNA cloning vector comprising: (a) a functional origin of replication-containing restriction fragment of plasmid pEL103, (b) one or more DNA segments that convey resistance to at least one antibiotic when transformed into a sensitive restrictionless host cell, said host cell being susceptible to transformation, cell division, and culture, and (c) a functional replicon-containing and antibiotic resistance-conferring restriction fragment of an E. coli plasmid.
Claim 33
Dependent Claim
65 words
A recombinant DNA cloning vector comprising: (a) a functional origin of replication-containing restriction fragment of plasmid pEL103, (b) one or more DNA segments that convey resistance to at least one antibiotic when transformed into a sensitive restrictionless host cell, said host cell being susceptible to transformation, cell division, and culture, and (c) a functional replicon-containing and antibiotic resistance-conferring restriction fragment of an E. coli plasmid.
Claim 34
Dependent Claim
13 words
The plasmid of claim 29 wherein the E. Coli plasmid is plasmid pBR322.
Claim 35
Dependent Claim
9 words
The plasmid of claim 30 which is plasmid pEL122.
Claim 36
Dependent Claim
14 words
A transformed restrictionless host cell comprising a recombinant DNA cloning vector of claim 1.
Claim 37
Dependent Claim
26 words
The transformed host cell of claim 32 which is selected from the group consisting of restrictionless cells of Streptomyces, Streptosporangium, Actinoplanes, Nocardia, Micromonospora, Bacillus, and Staphylococcus.
Claim 38
Dependent Claim
11 words
The transformed host cell of claim 33 which is a Streptomyces.
Claim 39
Dependent Claim
11 words
The transformed host cell of claim 33 which is a Streptosporangium.
Claim 40
Dependent Claim
11 words
The transformed host cell of claim 33 which is a Actinoplanes.
Claim 41
Dependent Claim
11 words
The transformed host cell of claim 33 which is a Nocardia.
Claim 42
Dependent Claim
11 words
The transformed host cell of claim 33 which is a Micromonospora.
Claim 43
Dependent Claim
11 words
The transformed host cell of claim 33 which is a Bacillus.
Claim 44
Dependent Claim
11 words
The transformed host cell of claim 33 which is a Staphylococcus.
Claim 45
Dependent Claim
39 words
The transformed host cell of claim 32 in which the recombinant DNA cloning vector is selected from the group consisting of plasmids pEL107, pEL105, pEL109, pEL110, pEL113, pEL114, pEL115, pEL116, pEL108, pEL104, pEL111, pEL112, pEL117, pEL118, pEL119, and pEL120.
Claim 46
Dependent Claim
11 words
The transformed host cell of claim 41 which is restrictionless Streptomyces.
Claim 47
Dependent Claim
9 words
The Streptomyces of claim 42 which is Streptomyces fradiae.
Claim 48
Dependent Claim
9 words
The Streptomyces of claim 42 which is Streptomyces coelicolor.
Claim 49
Dependent Claim
11 words
The transformed host cell of claim 42 which is Streptomyces ambofaciens/pEL107.
Claim 50
Dependent Claim
11 words
The transformed host cell of claim 42 which is Streptomyces ambofaciens/pEL105.
Claim 51
Dependent Claim
11 words
The transformed host cell of claim 42 which is Streptomyces ambofaciens/pEL109.
Claim 52
Dependent Claim
11 words
The transformed host cell of claim 42 which is Streptomyces ambofaciens/pEL110.
Claim 53
Dependent Claim
11 words
The tranformed host cell of claim 42 which is Streptomyces ambofaciens/pEL113.
Claim 54
Dependent Claim
11 words
The transformed host cell of claim 42 which is Streptomyces ambofaciens/pEL114.
Claim 55
Dependent Claim
11 words
The transformed host cell of claim 42 which is Streptomyces ambofaciens/pEL115.
Claim 56
Dependent Claim
11 words
The transformed host cell of claim 42 which is Streptomyces ambofaciens/pEL116.
Claim 57
Dependent Claim
11 words
The transformed host cell of claim 42 which is Streptomyces ambofaciens/pEL108.
Claim 58
Dependent Claim
11 words
The transformed host cell of claim 42 which is Streptomyces ambofaciens/pEL104.
Claim 59
Dependent Claim
11 words
The transformed host cell of claim 42 which is Streptomyces ambofaciens/pEL111.
Claim 60
Dependent Claim
11 words
The transformed host cell of claim 42 which is Streptomyces ambofaciens/pEL112.
Claim 61
Dependent Claim
11 words
The transformed host cell of claim 42 which is Streptomyces ambofaciens/pEL117.
Claim 62
Dependent Claim
11 words
The transformed host cell of claim 42 which is Streptomyces ambofaciens/pEL118.
Claim 63
Dependent Claim
11 words
The transformed host cell of claim 42 which is Streptomyces ambofaciens/pEL119.
Claim 64
Dependent Claim
11 words
The transformed host cell of claim 42 which is Streptomyces ambofaciens/pEL120.
Claim 65
Dependent Claim
14 words
A transformed restrictionless host cell comprising a recombinant DNA cloning vector of claim 29.
Claim 66
Dependent Claim
24 words
The transformed host cell of claim 61 wherein the replicon-containing and resistance-conferring restriction fragment in the vector is a restriction fragment of plasmid pBR322.
Claim 67
Dependent Claim
11 words
The transformed host cell of claim 61 which is Streptomyces ambofaciens/pEL121.
Claim 68
Dependent Claim
11 words
The transformed host cell of claim 61 which is restrictionless Streptomyces.
Claim 69
Dependent Claim
11 words
The transformed host cell of claim 61 which is E. coli.
Claim 70
Dependent Claim
13 words
The transformed host cell of claim 61 which is E. coli K12 HB101/pEL121.
Claim 71
Dependent Claim
13 words
The transformed host cell of claim 61 which is E. coli K12 HB101/pEL122.
Claim 72
Dependent Claim
17 words
The recombinant DNA cloning vector of claim 1 wherein one DNA segment conveys resistance to antibiotic erythromycin.
Claim 73
Dependent Claim
20 words
The recombinant DNA cloning vector of claim 68 in which the DNA segment is a restriction fragment of plasmid pIJ43.
Claim 74
Dependent Claim
27 words
The recombinant DNA cloning vector of claim 1 which is selected from the group consisting of plasmid pFJ124, pFJ144, pFJ145, pFJ146, pFJ147, pFJ148, pFJ149, pFJ152 and pFJ159.
Claim 75
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 70 which is plasmid pFJ124.
Claim 76
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 70 which is plasmid pFJ144.
Claim 77
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 70 which is plasmid pFJ146.
Claim 78
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 70 which is plasmid pFJ147.
Claim 79
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 70 which is plasmid pFJ148.
Claim 80
Dependent Claim
17 words
The recombinant DNA cloning vector of claim 29 wherein a DNA segment conveys resistance to antibiotic erythromycin.
Claim 81
Dependent Claim
20 words
The recombinant DNA cloning vector of claim 76 in which the DNA segment is a restriction fragment of plasmid pIJ43.
Claim 82
Dependent Claim
33 words
The recombinant DNA cloning vector of claim 29 which is selected from the group consisting of plasmid pFJ150, pFJ151, pFJ153, pFJ155, pFJ156, pFJ157, pFJ158, pFJ160, pFJ161, pFJ162, pFJ163, pFJ177, pFJ178, pFJ179 and pFJ180.
Claim 83
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 78 which is plasmid pFJ150.
Claim 84
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 78 which is plasmid pFJ153.
Claim 85
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 78 which is plasmid pFJ155.
Claim 86
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 78 which is plasmid pFJ160.
Claim 87
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 78 which is plasmid pFJ179.
Claim 88
Dependent Claim
14 words
A transformed restrictionless host cell comprising a recombinant DNA cloning vector of claim 70.
Claim 89
Dependent Claim
10 words
The transformed host cell of claim 84 which is Streptomyces.
Claim 90
Dependent Claim
11 words
The transformed host cell of claim 84 which is Streptomyces ambofaciens/pFJ124.
Claim 91
Dependent Claim
11 words
The transformed host cell of claim 84 which is Streptomyces ambofaciens/pFJ144.
Claim 92
Dependent Claim
11 words
The transformed host cell of claim 84 which is Streptomyces ambofaciens/pFJ146.
Claim 93
Dependent Claim
11 words
The transformed host cell of claim 84 which is Streptomyces ambofaciens/pFJ147.
Claim 94
Dependent Claim
11 words
The transformed host cell of claim 84 which is Streptomyces ambofaciens/pFJ148.
Claim 95
Dependent Claim
14 words
A transformed restrictionless host cell comprising a recombinant DNA cloning vector of claim 78.
Claim 96
Dependent Claim
13 words
The transformed host cell of claim 91 which is E. coli K12 HB101/pFJ150.
Claim 97
Dependent Claim
13 words
The transformed host cell of claim 91 which is E. coli K12 HB101/pFJ153.
Claim 98
Dependent Claim
13 words
The transformed host cell of claim 91 which is E. coli K12 HB101/pFJ155.
Claim 99
Dependent Claim
13 words
The transformed host cell of claim 91 which is E. coli K12 HB101/pFJ160.
Claim 100
Dependent Claim
13 words
The transformed host cell of claim 91 which is E. coli K12 HB101/pFJ179.
Claim 101
Dependent Claim
35 words
A transformed restrictionless host cell which is Streptomyces R/R 1 wherein R is ambofaciens, griseofuscus, lividans, fradiae, aureofaciens, tenebrarius or cinnamonensis and wherein R 1 independently is a recombinant DNA cloning vector of claim 70.
References Cited (Backward Citations)
WO1979001169A1
Cited
US4273875A
Cited
GB2048894A
Cited
US4340674A
Cited
US4332898A
Cited
US4332900A
Cited
US4338400A
Cited
US4342829A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-04-22 | 1988-10-22 | 1989-04-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-04-22 | 1992-10-22 | 1993-04-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-04-22 | 1996-10-22 | 1997-04-23 | $7700.00 | Window Closed / Lapsed |