US5334513A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for immunochromatographic analysis
Assignee: Siemens Healthcare Diagnostics GmbH Germany
•
Filing: 1992-09-03
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Granted: 1994-08-02
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Expires: 2012-09-03
Abstract
Abstract Methods are disclosed for conducting assays. One such method comprises providing in combination a first bibulous member zone ("first zone") and a liquid medium containing a component. The first zone has non-diffusively bound thereto a reagent interreactive with the component. Conditions are selected wherein the liquid medium and at least a portion of the component contained therein traverse all of the first zone and migrate by capillary migration into a second bibulous member zone ("second zone"). The second zone is of a different composition than the first zone and is incapable of specifically binding the component except when an analyte is to be detected and the method further includes causing a reagent to become bound to the first bibulous member zone in relation to the amount of analyte present. The distance the component has migrated into the second zone or the difference in the distances the medium and the component have migrated into the second zone is determined, the distance or the difference being related to the amount of the component in the liquid medium or the amount of the reagent.
Inventors
- 👤 Carl N. Skold
- 👤 Armen B. Shanafelt
- 👤 Vartan Ghazarossian
- 👤 Edwin F. Ullman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 11:10
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54388; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/97; Y10S435/975; Y10T; Y10T436/00; Y10T436/19
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
148 words
A method for determining the amount of a component in a liquid medium or a reagent, which method comprises: contacting a first bibulous member zone ("first zone") with a liquid medium containing a component, said first zone having non-diffusively bound therein a reagent interreactive with said component, under conditions wherein said liquid medium and at least a portion of said component contained therein traverse all of said first zone and migrate by capillary migration into a second bibulous member zone ("second zone") of different composition than said first zone and incapable of specifically binding said component and measuring the distance said component has migrated into said second zone or the difference in the distances said medium and said component have migrated into said second zone, said distance or said difference being related to the amount of said component in said liquid medium or the amount of said reagent.
Claim 2
Independent Claim
148 words
A method for determining the amount of a component in a liquid medium or a reagent, which method comprises: contacting a first bibulous member zone ("first zone") with a liquid medium containing a component, said first zone having non-diffusively bound therein a reagent interreactive with said component, under conditions wherein said liquid medium and at least a portion of said component contained therein traverse all of said first zone and migrate by capillary migration into a second bibulous member zone ("second zone") of different composition than said first zone and incapable of specifically binding said component and measuring the distance said component has migrated into said second zone or the difference in the distances said medium and said component have migrated into said second zone, said distance or said difference being related to the amount of said component in said liquid medium or the amount of said reagent.
Claim 3
Dependent Claim
52 words
The method of claim 1 wherein, prior to said contacting, said reagent is caused to non-diffusively bind to said first zone by contacting said first zone with a solution containing said reagent, and said difference or said distance is related to the amount of said reagent that binds to said first zone.
Claim 4
Dependent Claim
20 words
The method of claim 1 wherein said first zone and said second zone are integral to a single bibulous member.
Claim 5
Dependent Claim
49 words
The method of claim 1 wherein said first zone is integral to a first bibulous member and said second zone is integral to a second bibulous member and said first zone is brought into liquid receiving relationship with said second zone during said assay and prior to said contacting.
Claim 6
Dependent Claim
20 words
The method of claim 1 wherein said component and said reagent are each a member of a specific binding pair.
Claim 7
Dependent Claim
14 words
The method of claim 1 wherein said component and said reagent are chemically interreactive.
Claim 8
Dependent Claim
12 words
The method of claim 1 wherein said component is an inorganic ion.
Claim 9
Dependent Claim
22 words
The method of claim 1 wherein said component is an enzyme labeled ligand analog and said reagent is antibody for said ligand.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-08-01 | 1998-01-31 | 1998-08-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-08-01 | 2002-01-31 | 2002-08-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-08-01 | 2006-01-31 | 2006-08-02 | $7700.00 | Window Closed / Lapsed |