US5468647A
Expired / Lapsed
US
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Method for immunochromatographic analysis
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1994-09-01
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Granted: 1995-11-21
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Expires: 2014-09-01
Abstract
Abstract A method is disclosed for conducting an assay for an analyte. The method comprises causing a specific binding pair member in a first aqueous medium to become bound to a first bibulous member by contacting a portion of the first bibulous member with the medium. The first bibulous member is in liquid receiving relationship with an absorbent member. The contacting is carried out under conditions wherein the first medium traverses the first bibulous member and at least a portion of the absorbent member by capillary action. The method further comprises causing the first bibulous member to come into liquid receiving relationship with a second bibulous member. A reagent in a second aqueous medium is absorbed by and preferably becomes non-diffusively bound to the second bibulous member in relation to the presence of analyte in the first medium. This is accomplished by contacting a portion of the first bibulous member with the second aqueous medium under conditions wherein the second aqueous medium traverses the first bibulous member by capillary action and at least a portion of the second bibulous member. Next, the presence of the reagent on at least a portion of the second bibulous member is determined.
Inventors
- 👤 Carl N. Skold
- 👤 Armen B. Shanafelt
- 👤 Vartan Ghazarossian
- 👤 Edwin F. Ullman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 17:58
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54388; Y; Y10; Y10S; Y10S435/00; Y10S435/97; Y10S435/971; Y10S435/972; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/815; Y10S436/816; Y10S436/819; Y10S436/822
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
190 words
A device for analyzing for the presence of an analyte, which device comprises: (a) a first bibulous member, at least a portion thereof having a first member of a specific binding pair non-diffusively bound thereto and capable of binding said analyte with the proviso that, when said analyte is not capable of binding to said first member of the specific binding pair, a first reagent capable of binding said analyte and said first member of the specific binding pair is present in relation to said analyte, (b) an absorbent member in terminable liquid receiving relationship with said first bibulous member, (c) a second bibulous member, at least a portion thereof having a second member of a specific binding pair non-diffusively bound thereto, said second bibulous member being adapted to establish a liquid receiving relationship with said first bibulous member, said second member being capable of binding a second reagent in relation to the amount of analyte and (d) means for terminating the liquid receiving relationship between said first bibulous member and said absorbent member and establishing a liquid receiving relationship between said first bibulous member and said second bibulous member.
Claim 2
Independent Claim
190 words
A device for analyzing for the presence of an analyte, which device comprises: (a) a first bibulous member, at least a portion thereof having a first member of a specific binding pair non-diffusively bound thereto and capable of binding said analyte with the proviso that, when said analyte is not capable of binding to said first member of the specific binding pair, a first reagent capable of binding said analyte and said first member of the specific binding pair is present in relation to said analyte, (b) an absorbent member in terminable liquid receiving relationship with said first bibulous member, (c) a second bibulous member, at least a portion thereof having a second member of a specific binding pair non-diffusively bound thereto, said second bibulous member being adapted to establish a liquid receiving relationship with said first bibulous member, said second member being capable of binding a second reagent in relation to the amount of analyte and (d) means for terminating the liquid receiving relationship between said first bibulous member and said absorbent member and establishing a liquid receiving relationship between said first bibulous member and said second bibulous member.
Claim 3
Dependent Claim
24 words
A kit for conducting an assay, which comprises in packaged combination: (a) the device of claim 1 and (b) reagents for conducting an assay.
Claim 4
Independent Claim
205 words
A device for analyzing for the presence of an analyte, which device comprises: (a) a first bibulous strip having an end portion and a second portion having a first member of a specific binding pair non-diffusively bound thereto and capable of binding said analyte with the proviso that, when said analyte is not capable of binding to said first member of the specific binding pair, a first reagent capable of binding said analyte and said first member of the specific binding pair is present in relation to said analyte, (b) an absorbent strip in terminable liquid receiving relationship with said second portion of said first bibulous strip, (c) a second bibulous strip, at least a portion thereof having a second member of a specific binding pair non-diffusively bound thereto, said second bibulous strip being adapted to establish a liquid receiving relationship with said first bibulous strip, said second member being capable of binding a second reagent in relation to the amount of analyte and (d) means for terminating the liquid receiving relationship between said first bibulous strip and said absorbent strip and establishing a liquid receiving relationship between said second portion of said first bibulous strip and an end portion of said second bibulous strip.
Claim 5
Independent Claim
205 words
A device for analyzing for the presence of an analyte, which device comprises: (a) a first bibulous strip having an end portion and a second portion having a first member of a specific binding pair non-diffusively bound thereto and capable of binding said analyte with the proviso that, when said analyte is not capable of binding to said first member of the specific binding pair, a first reagent capable of binding said analyte and said first member of the specific binding pair is present in relation to said analyte, (b) an absorbent strip in terminable liquid receiving relationship with said second portion of said first bibulous strip, (c) a second bibulous strip, at least a portion thereof having a second member of a specific binding pair non-diffusively bound thereto, said second bibulous strip being adapted to establish a liquid receiving relationship with said first bibulous strip, said second member being capable of binding a second reagent in relation to the amount of analyte and (d) means for terminating the liquid receiving relationship between said first bibulous strip and said absorbent strip and establishing a liquid receiving relationship between said second portion of said first bibulous strip and an end portion of said second bibulous strip.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-11-20 | 1999-05-22 | 1999-11-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-11-20 | 2003-05-22 | 2003-11-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-11-20 | 2007-05-22 | 2007-11-21 | $7700.00 | Window Closed / Lapsed |