US5705348A
Expired / Lapsed
US
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Nucleic acid mediated electron transfer
Assignee: California Institute of Technology
•
Filing: 1996-09-06
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Granted: 1998-01-06
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Expires: 2016-09-06
Abstract
Abstract The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
Inventors
- 👤 Thomas J. Meade
- 👤 Jon Faiz Kayyem
- 👤 Scott E. Fraser
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 15:12
CPC Classifications
C; C07; C07H; C07H23/00; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6818; C12Q1/6825; C12Q1/6827
Claims Analysis
Total Claims: 40
Claim 1
Independent Claim
73 words
A single-stranded nucleic acid containing one or multiple electron donor moieties and one or multiple electron acceptor moieties, wherein said electron donor and acceptor moieties are transition metal complexes covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said nucleic acid, and wherein electron transfer can occur between said electron donor and acceptor moieties when said single stranded nucleic acid is hybridized to a target sequence.
Claim 2
Dependent Claim
73 words
A single-stranded nucleic acid containing one or multiple electron donor moieties and one or multiple electron acceptor moieties, wherein said electron donor and acceptor moieties are transition metal complexes covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said nucleic acid, and wherein electron transfer can occur between said electron donor and acceptor moieties when said single stranded nucleic acid is hybridized to a target sequence.
Claim 3
Dependent Claim
81 words
A single-stranded nucleic acid containing one or multiple electron donor moieties and one or multiple electron acceptor moieties, wherein one of the electron donor or acceptor moieties is an electrode and the other is a transition metal complex covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said nucleic acid, and wherein electron transfer can occur between said electron donor and acceptor moieties when said single stranded nucleic acid hybridizes to a target sequence.
Claim 4
Dependent Claim
81 words
A single-stranded nucleic acid containing one or multiple electron donor moieties and one or multiple electron acceptor moieties, wherein one of the electron donor or acceptor moieties is an electrode and the other is a transition metal complex covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said nucleic acid, and wherein electron transfer can occur between said electron donor and acceptor moieties when said single stranded nucleic acid hybridizes to a target sequence.
Claim 5
Dependent Claim
22 words
A single stranded nucleic acid according to claim 1 or 2 wherein said attachment is at the 3' position of said ribose.
Claim 6
Dependent Claim
22 words
A single stranded nucleic acid according to claim 1 or 2 wherein said attachment is at the 2' position of said ribose.
Claim 7
Dependent Claim
29 words
A single stranded nucleic acid according to claim 1 or 2 wherein there are 5 unconjugated sigma bonds between said transition metal and the base attached to said ribose.
Claim 8
Dependent Claim
29 words
A single stranded nucleic acid according to claim 1 or 2 wherein there are 4 unconjugated sigma bonds between said transition metal and the base attached to said ribose.
Claim 9
Dependent Claim
96 words
A composition comprising a first single stranded nucleic acid containing one or multiple electron donor moieties and a second single stranded nucleic acid containing one or multiple electron acceptor moieties, wherein said electron donor and acceptor moieties are transition metal complexes covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said first and second single stranded nucleic acids, and wherein electron transfer can occur between said electron donor and said electron acceptor moieties when said first and second single stranded nucleic acids are adjacently hybridized to a target domain.
Claim 10
Dependent Claim
96 words
A composition comprising a first single stranded nucleic acid containing one or multiple electron donor moieties and a second single stranded nucleic acid containing one or multiple electron acceptor moieties, wherein said electron donor and acceptor moieties are transition metal complexes covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said first and second single stranded nucleic acids, and wherein electron transfer can occur between said electron donor and said electron acceptor moieties when said first and second single stranded nucleic acids are adjacently hybridized to a target domain.
Claim 11
Dependent Claim
91 words
A composition comprising a first single stranded nucleic acid containing one or multiple electron donor moieties and a second single stranded nucleic acid containing one or multiple electron acceptor moieties, wherein one of the electron transfer moieties is an electrode and the other is a transition metal complex covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone, and wherein electron transfer can occur between said electron donor and said electron acceptor moieties when said single stranded nucleic acids are adjacently hybridized to a target sequence.
Claim 12
Dependent Claim
91 words
A composition comprising a first single stranded nucleic acid containing one or multiple electron donor moieties and a second single stranded nucleic acid containing one or multiple electron acceptor moieties, wherein one of the electron transfer moieties is an electrode and the other is a transition metal complex covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone, and wherein electron transfer can occur between said electron donor and said electron acceptor moieties when said single stranded nucleic acids are adjacently hybridized to a target sequence.
Claim 13
Dependent Claim
19 words
A composition according to claim 7 or 8 wherein said attachment is at the 3' position of said ribose.
Claim 14
Dependent Claim
19 words
A composition according to claim 7 or 8 wherein said attachment is at the 2' position of said ribose.
Claim 15
Dependent Claim
26 words
A composition according to claim 7 or 8 wherein there are 5 unconjugated sigma bonds between said transition metal and the base attached to said ribose.
Claim 16
Dependent Claim
26 words
A composition according to claim 7 or 8 wherein there are 4 unconjugated sigma bonds between said transition metal and the base attached to said ribose.
Claim 17
Independent Claim
102 words
A method of detecting a target sequence in a nucleic acid sample comprising: a) hybridizing a single stranded nucleic acid containing one or multiple electron donor moieties and one or multiple electron acceptor moieties to said target sequence to form a hybridization complex, wherein said electron donor and electron acceptor moieties are transition metal complexes covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said nucleic acids; and b) detecting electron transfer between said electron donor and acceptor moieties in the hybridization complex as an indicator of the presence or absence of said target sequence.
Claim 18
Independent Claim
102 words
A method of detecting a target sequence in a nucleic acid sample comprising: a) hybridizing a single stranded nucleic acid containing one or multiple electron donor moieties and one or multiple electron acceptor moieties to said target sequence to form a hybridization complex, wherein said electron donor and electron acceptor moieties are transition metal complexes covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said nucleic acids; and b) detecting electron transfer between said electron donor and acceptor moieties in the hybridization complex as an indicator of the presence or absence of said target sequence.
Claim 19
Independent Claim
113 words
A method of detecting a target sequence in a nucleic acid sample comprising: a) hybridizing a single stranded nucleic acid containing one or multiple electron donor moieties and one or multiple electron acceptor moieties to said target sequence to form a hybridization complex, wherein one of said electron donor or acceptor moieties is an electrode and the other is a transition metal complex covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said nucleic acid; and b) detecting electron transfer between said electron donor moieties and said electron acceptor moieties in the hybridization complex as an indicator of the presence or absence of said target sequence.
Claim 20
Independent Claim
113 words
A method of detecting a target sequence in a nucleic acid sample comprising: a) hybridizing a single stranded nucleic acid containing one or multiple electron donor moieties and one or multiple electron acceptor moieties to said target sequence to form a hybridization complex, wherein one of said electron donor or acceptor moieties is an electrode and the other is a transition metal complex covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said nucleic acid; and b) detecting electron transfer between said electron donor moieties and said electron acceptor moieties in the hybridization complex as an indicator of the presence or absence of said target sequence.
Claim 21
Dependent Claim
19 words
A method according to claim 13 or 14 wherein said attachment is at the 2' position of said ribose.
Claim 22
Dependent Claim
19 words
A method according to claim 13 or 14 wherein said attachment is at the 3' position of said ribose.
Claim 23
Dependent Claim
26 words
A method according to claim 13 or 14 wherein there are 5 unconjugated sigma bonds between said transition metal and the base attached to said ribose.
Claim 24
Dependent Claim
26 words
A method according to claim 13 or 14 wherein there are 4 unconjugated sigma bonds between said transition metal and the base attached to said ribose.
Claim 25
Independent Claim
146 words
A method of detecting a target sequence in a nucleic acid wherein said target sequence comprises a first target domain and a second target domain adjacent to said first target domain, said method comprising: a) hybridizing a first nucleic acid containing one or multiple electron donor moieties to said first target domain; b) hybridizing a second nucleic acid containing one or multiple electron acceptor moieties to said second target domain, wherein said electron donor and electron acceptor moieties are transition metal complexes covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said nucleic acids; and c) detecting electron transfer between said electron donor and acceptor moieties while said first and second nucleic acids are hybridized to said first and second target domains as an indicator of the presence or absence of said target sequence in said nucleic acid sample.
Claim 26
Independent Claim
146 words
A method of detecting a target sequence in a nucleic acid wherein said target sequence comprises a first target domain and a second target domain adjacent to said first target domain, said method comprising: a) hybridizing a first nucleic acid containing one or multiple electron donor moieties to said first target domain; b) hybridizing a second nucleic acid containing one or multiple electron acceptor moieties to said second target domain, wherein said electron donor and electron acceptor moieties are transition metal complexes covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said nucleic acids; and c) detecting electron transfer between said electron donor and acceptor moieties while said first and second nucleic acids are hybridized to said first and second target domains as an indicator of the presence or absence of said target sequence in said nucleic acid sample.
Claim 27
Independent Claim
154 words
A method of detecting a target sequence in a nucleic acid wherein said target sequence comprises a first target domain and a second target domain adjacent to said first target domain, said method comprising: a) hybridizing a first nucleic acid containing one or multiple electron donor moieties to said first target domain; b) hybridizing a second nucleic acid containing one or multiple electron acceptor moieties to said second target domain, wherein one of said electron donor and acceptor moieties is an electrode and the other is a transition metal complex covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said nucleic acid; and c) detecting electron transfer between said electron donor and acceptor moieties while said first and second nucleic acids are hybridized to said first and second target domains as an indicator of the presence or absence of said target sequence in said nucleic acid sample.
Claim 28
Independent Claim
154 words
A method of detecting a target sequence in a nucleic acid wherein said target sequence comprises a first target domain and a second target domain adjacent to said first target domain, said method comprising: a) hybridizing a first nucleic acid containing one or multiple electron donor moieties to said first target domain; b) hybridizing a second nucleic acid containing one or multiple electron acceptor moieties to said second target domain, wherein one of said electron donor and acceptor moieties is an electrode and the other is a transition metal complex covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said nucleic acid; and c) detecting electron transfer between said electron donor and acceptor moieties while said first and second nucleic acids are hybridized to said first and second target domains as an indicator of the presence or absence of said target sequence in said nucleic acid sample.
Claim 29
Dependent Claim
19 words
A method according to claim 19 or 20 wherein said attachment is at the 2' position of said ribose.
Claim 30
Dependent Claim
19 words
A method according to claim 19 or 20 wherein said attachment is at the 3' position of said ribose.
Claim 31
Dependent Claim
26 words
A method according to claim 19 or 20 wherein there are 5 unconjugated sigma bonds between said transition metal and the base attached to said ribose.
Claim 32
Dependent Claim
26 words
A method according to claim 19 or 20 wherein there are 4 unconjugated sigma bonds between said transition metal and the base attached to said ribose.
Claim 33
Independent Claim
164 words
A method of detecting a target sequence in a nucleic acid wherein said target sequence comprises a first target domain, a second target domain, and an intervening target domain between said first and said second target domains, said method comprising: a) hybridizing a first nucleic acid containing one or multiple electron donor moieties to said first target domain; b) hybridizing a second nucleic acid containing one or multiple electron acceptor moieties to said second target domain, wherein said electron donor or acceptor moieties are transition metal complexes covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said nucleic acid; c) hybridizing an intervening nucleic acid to said intervening target domain; and d) detecting electron transfer between said electron donor and acceptor moieties while said first, second and intervening nucleic acids are hybridized to said first, second and intervening target domains as an indicator of the presence or absence of said target sequence in said nucleic acid sample.
Claim 34
Independent Claim
164 words
A method of detecting a target sequence in a nucleic acid wherein said target sequence comprises a first target domain, a second target domain, and an intervening target domain between said first and said second target domains, said method comprising: a) hybridizing a first nucleic acid containing one or multiple electron donor moieties to said first target domain; b) hybridizing a second nucleic acid containing one or multiple electron acceptor moieties to said second target domain, wherein said electron donor or acceptor moieties are transition metal complexes covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said nucleic acid; c) hybridizing an intervening nucleic acid to said intervening target domain; and d) detecting electron transfer between said electron donor and acceptor moieties while said first, second and intervening nucleic acids are hybridized to said first, second and intervening target domains as an indicator of the presence or absence of said target sequence in said nucleic acid sample.
Claim 35
Independent Claim
176 words
A method of detecting a target sequence in a nucleic acid wherein said target sequence comprises a first target domain, a second target domain, and an intervening target domain between said first and said second target domains, said method comprising: a) hybridizing a first nucleic acid containing one or multiple electron donor moieties to said first target domain; b) hybridizing a second nucleic acid containing one or multiple electron acceptor moieties to said second target domain, wherein one of said electron donor and acceptor moieties is an electrode and the other is a transition metal complex covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said nucleic acid; c) hybridizing an intervening nucleic acid to said intervening target domain; and d) detecting electron transfer between said electron donor moiety and said electron acceptor moiety while said first, second and intervening nucleic acids are hybridized to said first, second and intervening target domains as an indicator of the presence or absence of said target sequence in said nucleic acid sample.
Claim 36
Independent Claim
176 words
A method of detecting a target sequence in a nucleic acid wherein said target sequence comprises a first target domain, a second target domain, and an intervening target domain between said first and said second target domains, said method comprising: a) hybridizing a first nucleic acid containing one or multiple electron donor moieties to said first target domain; b) hybridizing a second nucleic acid containing one or multiple electron acceptor moieties to said second target domain, wherein one of said electron donor and acceptor moieties is an electrode and the other is a transition metal complex covalently attached to the 2' or 3' position of a ribose of the ribose-phosphate backbone of said nucleic acid; c) hybridizing an intervening nucleic acid to said intervening target domain; and d) detecting electron transfer between said electron donor moiety and said electron acceptor moiety while said first, second and intervening nucleic acids are hybridized to said first, second and intervening target domains as an indicator of the presence or absence of said target sequence in said nucleic acid sample.
Claim 37
Dependent Claim
19 words
A method according to claim 25 or 26 wherein said attachment is at the 2' position of said ribose.
Claim 38
Dependent Claim
19 words
A method according to claim 25 or 26 wherein said attachment is at the 3' position of said ribose.
Claim 39
Dependent Claim
26 words
A method according to claim 25 or 26 wherein there are 5 unconjugated sigma bonds between said transition metal and the base attached to said ribose.
Claim 40
Dependent Claim
26 words
A method according to claim 25 or 26 wherein there are 4 unconjugated sigma bonds between said transition metal and the base attached to said ribose.
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