Patent US4868108A successfully refreshed from Google Patents.
US4868108A
Expired / Lapsed
US
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Multiple-antibody detection of antigen
Assignee: Hygeia Sciences Inc
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Filing: 1985-12-12
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Granted: 1989-09-19
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Expires: 2005-12-12
Abstract
Abstract An assay employing two antibodies. A microporous carrier supports a plurality of layers, in which conjugates form between test antigens and antibodies. Quantitative results are obtained from an immobilized antibody within an indicating substrate.
Inventors
- 👤 Izak Bahar
- 👤 Francis Cole
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 17:57
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/538; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54388; G01N33/54389; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S436/00; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
133 words
An apparatus for indicating a test antigen in a solution, comprising: (a) a porous support having a plurality of regions; (b) a first antibody bound to an enzyme to form an enzyme-linked first antibody; said enzyme-linked first antibody being disposed within one of said regions; (c) a second antibody immobilized within a region other than that of the enzyme-linked first antibody; and (d) a substrate disposed in a region other than the region of said enzyme-linked first antibody, or the region of said second antibody, said substrate being capable of reacting with said enzyme to yield an indication of test antigen; said regions being disposed such that the solution flows through the region of said enzyme-linked first antibody to the region of said second antibody and then to the region of said substrate.
Claim 2
Independent Claim
133 words
An apparatus for indicating a test antigen in a solution, comprising: (a) a porous support having a plurality of regions; (b) a first antibody bound to an enzyme to form an enzyme-linked first antibody; said enzyme-linked first antibody being disposed within one of said regions; (c) a second antibody immobilized within a region other than that of the enzyme-linked first antibody; and (d) a substrate disposed in a region other than the region of said enzyme-linked first antibody, or the region of said second antibody, said substrate being capable of reacting with said enzyme to yield an indication of test antigen; said regions being disposed such that the solution flows through the region of said enzyme-linked first antibody to the region of said second antibody and then to the region of said substrate.
Claim 3
Independent Claim
142 words
An apparatus for indicating a test antigen in a solution, comprising: (a) a porous support having a plurality of regions; (b) a first antibody bound to an enzyme to form an enzyme-linked first antibody; said enzyme-linked first antibody being disposed within one of said regions; (c) an antigen which will immunologically bind to said enzyme-linked first antibody, said antigen being disposed in a region other than the region of said enzyme-linked first antibody; (d) a second antibody immobilized within a region other than the region of said enzyme-linked first antibody, or the region of said antigen; (e) a substrate disposed in the region of said second antibody; said regions being disposed such that the solution flows through the region of said enzyme-linked first antibody to the region of said antigen, and then to the region of said second antibody and said substrate.
Claim 4
Independent Claim
142 words
An apparatus for indicating a test antigen in a solution, comprising: (a) a porous support having a plurality of regions; (b) a first antibody bound to an enzyme to form an enzyme-linked first antibody; said enzyme-linked first antibody being disposed within one of said regions; (c) an antigen which will immunologically bind to said enzyme-linked first antibody, said antigen being disposed in a region other than the region of said enzyme-linked first antibody; (d) a second antibody immobilized within a region other than the region of said enzyme-linked first antibody, or the region of said antigen; (e) a substrate disposed in the region of said second antibody; said regions being disposed such that the solution flows through the region of said enzyme-linked first antibody to the region of said antigen, and then to the region of said second antibody and said substrate.
Claim 5
Dependent Claim
53 words
The apparatus of claim 1, wherein said first antibody is immunologically bound to an antigen and wherein said regions are disposed such that the solution flows through the region of said enzyme-linked first antibody bound to said antigen, to the region of said second antibody and then to the region of said substrate.
Claim 6
Dependent Claim
30 words
The apparatus of claim 3, wherein said enzyme bound to said first antibody bound to said antigen is glucose oxidase and glucose is present in said region of said substrate.
Claim 7
Dependent Claim
31 words
The apparatus of claim 3, wherein glucose oxidase is bound to said antigen which is immunologically bound to said first antibody and glucose is present in the region of said substrate.
Claim 8
Dependent Claim
53 words
The apparatus of claim 1, wherein said second antibody is immunologically bound to an antigen and wherein said regions are disposed such that the solution flows through the region of said enzyme-linked first antibody to the region of said second antibody bound to said antigen and then to the region of said substrate.
Claim 9
Dependent Claim
17 words
The apparatus of claim 1 or 2, wherein said regions are in the form of concentric circles.
Claim 10
Dependent Claim
18 words
The apparatus of claim 1 or 2, wherein said regions are in the form of consecutive transverse layers.
Claim 11
Dependent Claim
17 words
The apparatus of claim 1 or 2, wherein said regions are in the form of superimposed layers.
Claim 12
Dependent Claim
12 words
The apparatus of claim 1 or 2, wherein said substrate comprises tetramethylbenzidine.
Claim 13
Dependent Claim
13 words
The apparatus of claim 1 or 2, wherein said enzyme is horseradish peroxidase.
Claim 14
Dependent Claim
13 words
The apparatus of claim 1 or 2, wherein said enzyme is alkaline phosphatase.
Claim 15
Independent Claim
196 words
An apparatus for indicating the presence of test antigen in a solution comprising: (a) a bibulous microporous support having a plurality of distinct regions; (b) a first antibody disposed within one of said regions, said antibody being bound to an enzyme to form an enzyme-linked first antibody; (c) a second antibody disposed within the same region as that of said enzyme-linked first antibody, said second antibody being bound to glucose oxidase; (d) an antigen which will immunologically bind to said enzyme-linked first antibody and to said second antibody, said antigen being immobilized in a region other than that of said enzyme-linked first antibody and that of said second antibody; and (e) a substrate comprising glucose, said substrate being in a region other than that of said enzyme-linked first antibody and said second antibody and that of said antigen, said substrate being capable of reacting with said glucose oxidase and said enzyme to yield an indication of test antigen; said region being disposed such that said solution flows through the region of said enzyme-linked first antibody and the region of said second antibody, to the region of said antigen and then to the region of said substrate.
Claim 16
Independent Claim
196 words
An apparatus for indicating the presence of test antigen in a solution comprising: (a) a bibulous microporous support having a plurality of distinct regions; (b) a first antibody disposed within one of said regions, said antibody being bound to an enzyme to form an enzyme-linked first antibody; (c) a second antibody disposed within the same region as that of said enzyme-linked first antibody, said second antibody being bound to glucose oxidase; (d) an antigen which will immunologically bind to said enzyme-linked first antibody and to said second antibody, said antigen being immobilized in a region other than that of said enzyme-linked first antibody and that of said second antibody; and (e) a substrate comprising glucose, said substrate being in a region other than that of said enzyme-linked first antibody and said second antibody and that of said antigen, said substrate being capable of reacting with said glucose oxidase and said enzyme to yield an indication of test antigen; said region being disposed such that said solution flows through the region of said enzyme-linked first antibody and the region of said second antibody, to the region of said antigen and then to the region of said substrate.
Claim 17
Independent Claim
102 words
A method of determining the presence of an antigen in a test solution, comprising the steps of: (a) reacting the test solution with a first antibody bound to an enzyme and disposed on a porous support to form a first migrating solution comprising test solution and reconstituted first antibody bound to an enzyme; (b) reacting the first migrating solution of step (a) with a second antibody immobilized on said support to form a second migrating solution; and (c) reacting the second migrating solution of step (b) with a substrate capable of reacting with said enzyme and which is disposed on said support.
Claim 18
Independent Claim
102 words
A method of determining the presence of an antigen in a test solution, comprising the steps of: (a) reacting the test solution with a first antibody bound to an enzyme and disposed on a porous support to form a first migrating solution comprising test solution and reconstituted first antibody bound to an enzyme; (b) reacting the first migrating solution of step (a) with a second antibody immobilized on said support to form a second migrating solution; and (c) reacting the second migrating solution of step (b) with a substrate capable of reacting with said enzyme and which is disposed on said support.
Claim 19
Dependent Claim
21 words
The method of claim 14, wherein an antigen is bound to the first antibody prior to the reaction of step (a).
Claim 20
Dependent Claim
42 words
The method of claim 15, wherein glucose oxidase is bound to said antigen bound to the first antibody prior to the reaction of step (a); and glucose is provided to the region of said substrate prior to the reaction of step (c).
Claim 21
Dependent Claim
21 words
The method of claim 14, wherein an antigen is bound to the second antibody prior to the reaction of step (b).
Claim 22
Dependent Claim
16 words
The method of claim 14, wherein the enzyme bound to said first antibody is glucose oxidase.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-09-18 | 1993-03-20 | 1993-09-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-09-18 | 1997-03-20 | 1997-09-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-09-18 | 2001-03-20 | 2001-09-19 | $7700.00 | Window Closed / Lapsed |