Patent US4959307A successfully refreshed from Google Patents.
US4959307A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immunoseparating strip
Assignee: Abbott Laboratories
•
Filing: 1986-09-05
•
Granted: 1990-09-25
•
Expires: 2006-09-05
Abstract
Abstract A method and device for determining the presence of an analyte in a sample suspected of containing the analyte are disclosed. The method involves contacting a test solution containing the sample, an antibody for the analyte, and a conjugate of the analyte and a label with a contact portion of a strip of bibulous material capable of being traversed by the test solution through capillary action. The strip contains a first receptor capable of binding to the conjugate. The first receptor is non-diffusively bound to a situs on the strip separate from the contact portion of the strip. The strip further contains a second receptor capable of binding the antibody to the analyte between the situs and the contact portion. The second receptor is non-diffusively bound to the strip. At least a portion of the test solution is allowed to traverse the strip by capillary action and thereby contact the situs. The strip is exposed to a signal producing means capable of interacting with the label to produce a signal in relation to the amount of analyte in the test solution. The signal produced at the situs is then detected.
Inventors
- 👤 John D. Olson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 18:34
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54388; G01N33/94; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/97; Y10S435/973; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/815; Y10S436/816
Claims Analysis
Total Claims: 31
Claim 1
Independent Claim
209 words
A method for determining the presence of an analyte, that is capable of binding specifically to an antibody, in a sample suspected of containing said analyte, which comprises: (a) contacting, with a test solution containing said sample, antibodies to said analyte and a conjugate of said analyte and a label, a contact portion of a strip of bibulous material capable of being traversed by said test solution by capillary migration, said strip containing non-diffusively bound to a situs on said strip separated from said contact portion a first receptor capable of binding to said conjugate, the surface area of said situs being less than that of said strip, said strip further containing a second receptor capable of binding said antibodies to said analyte, said second receptor being non-diffusively bound to said strip between said situs and said contact portion, (b) allowing at least a portion of said test solution to traverse said strip by capillary migration and thereby contact said situs, (c) exposing said strip to a signal producing means capable of interacting with said label to produce a signal in relation to the amount of analyte in the test solution, and (d) detecting said signal at said situs and relating said signal to the presence of said analyte.
Claim 2
Independent Claim
209 words
A method for determining the presence of an analyte, that is capable of binding specifically to an antibody, in a sample suspected of containing said analyte, which comprises: (a) contacting, with a test solution containing said sample, antibodies to said analyte and a conjugate of said analyte and a label, a contact portion of a strip of bibulous material capable of being traversed by said test solution by capillary migration, said strip containing non-diffusively bound to a situs on said strip separated from said contact portion a first receptor capable of binding to said conjugate, the surface area of said situs being less than that of said strip, said strip further containing a second receptor capable of binding said antibodies to said analyte, said second receptor being non-diffusively bound to said strip between said situs and said contact portion, (b) allowing at least a portion of said test solution to traverse said strip by capillary migration and thereby contact said situs, (c) exposing said strip to a signal producing means capable of interacting with said label to produce a signal in relation to the amount of analyte in the test solution, and (d) detecting said signal at said situs and relating said signal to the presence of said analyte.
Claim 3
Dependent Claim
15 words
The method of claim 1 wherein said strip of bibulous material is a paper strip.
Claim 4
Dependent Claim
26 words
The method of claim 1 wherein said signal at said situs is compared with said signal at a portion of the strip other than said situs.
Claim 5
Dependent Claim
19 words
The method of claim 1 wherein said signal producing means comprises a substrate and said label is a catalyst.
Claim 6
Dependent Claim
24 words
The method of claim 4 wherein at least the portion of said strip containing said situs is immersed in a solution of said substrate.
Claim 7
Dependent Claim
36 words
The method of claim 4 wherein the contact portion of said strip is contacted with a solution of said substrate which is allowed to traverse said strip past said situs by means of the capillary migration.
Claim 8
Dependent Claim
12 words
The method of claim 1 wherein said first receptor is an antibody.
Claim 9
Dependent Claim
28 words
The method of claim 1 wherein said antibodies for said analyte are selected from the group consisting of antibodies for theopylline, antibodies for phenobarbital, and antibodies for quinidine.
Claim 10
Dependent Claim
14 words
The method of claim 1 wherein said second receptor is antibody for said antibodies.
Claim 11
Dependent Claim
11 words
The method of claim 1 wherein said label is an enzyme.
Claim 12
Dependent Claim
34 words
The method of claim 10 wherein a second enzyme is bound at said situs to said strip, the enzymes being related in that the product of one enzyme is the substrate of the other.
Claim 13
Dependent Claim
84 words
The method of claim 1 for determining a plurality of analytes in said sample, wherein (i) a plurality of conjugates, each comprising one of said analytes and a label, and (ii) a plurality of antibodies, each specific to one or more of said analytes, are combined with said sample, and a plurality of situses, non-contiguous to each other and to said contact portion of said strip, are employed each having non-diffusively bound thereto a different first receptor capable of binding to a different conjugate.
Claim 14
Dependent Claim
24 words
The method of claim 1 wherein said situs is a band transverse to the direction of traversal of said test solution along said strip.
Claim 15
Dependent Claim
40 words
The method of claim 1 wherein the signal produced at said situs has a sharp-edged distinctive pattern that provides a sharp contrast to a signal produced at adjacent sites on said strip when analyte is present in said test solution.
Claim 16
Dependent Claim
11 words
The method of claim 1 wherein said analyte is a drug.
Claim 17
Independent Claim
255 words
A method for determining the presence of one or more of a plurality of analytes, each of which is capable of binding specifically to an antibody, in a sample suspected of containing said analytes, which comprises: (a) contacting, with a test solution containing said sample, a plurality of antibodies each corresponding to one of said analytes and a plurality of conjugates, each comprising one of said analytes and a label, a contact portion of a strip of bibulous material capable of being traversed by said test solution by capillary migration, said strip containing one or more situses on said strip separate from said contact portion, each of said situses having non-diffusively bound thereto a first receptor capable of binding to one of said conjugates, the surface area of each of said situses being less than that of said strip, said strip further containing a second receptor capable of binding said plurality of antibodies to said analyte, said second receptor being non-diffusively bound to said strip between said situses and said contact portion, (b) allowing at least a portion of said test solution to traverse said strip by capillary migration and thereby contact said situses, (c) exposing said strip to a signal producing means capable of interacting with said label(s) to produce a signal at each situs in relation to the amount of each analyte in the test solution, and (d) detecting said signal at each of said situses and relating the signal at each of said situses to the presence of one or more analytes.
Claim 18
Independent Claim
255 words
A method for determining the presence of one or more of a plurality of analytes, each of which is capable of binding specifically to an antibody, in a sample suspected of containing said analytes, which comprises: (a) contacting, with a test solution containing said sample, a plurality of antibodies each corresponding to one of said analytes and a plurality of conjugates, each comprising one of said analytes and a label, a contact portion of a strip of bibulous material capable of being traversed by said test solution by capillary migration, said strip containing one or more situses on said strip separate from said contact portion, each of said situses having non-diffusively bound thereto a first receptor capable of binding to one of said conjugates, the surface area of each of said situses being less than that of said strip, said strip further containing a second receptor capable of binding said plurality of antibodies to said analyte, said second receptor being non-diffusively bound to said strip between said situses and said contact portion, (b) allowing at least a portion of said test solution to traverse said strip by capillary migration and thereby contact said situses, (c) exposing said strip to a signal producing means capable of interacting with said label(s) to produce a signal at each situs in relation to the amount of each analyte in the test solution, and (d) detecting said signal at each of said situses and relating the signal at each of said situses to the presence of one or more analytes.
Claim 19
Dependent Claim
15 words
The method of claim 16 wherein said strip of bibulous material is a paper strip.
Claim 20
Dependent Claim
26 words
The method of claim 16 wherein said signal at said situses is compared with said signal at a portion of the strip other than said situses.
Claim 21
Dependent Claim
20 words
The method of claim 16 wherein said signal producing means comprises a substrate and said label(s) is (are) a catalyst.
Claim 22
Dependent Claim
24 words
The method of claim 19 wherein at least the portion of said strip containing said situses is immersed in a solution of said substrate.
Claim 23
Dependent Claim
35 words
The method of claim 19 wherein the contact portion of said strip is contacted with a solution of said substrate which is allowed to traverse said strip past said situses by means of capillary migration.
Claim 24
Dependent Claim
11 words
The method of claim 16 wherein said first receptors are antibodies.
Claim 25
Dependent Claim
28 words
The method of claim 16 wherein said antibodies for said analyte are selected from the group consisting of antibodies for theopylline, antibodies for phenobarbital, and antibodies for quinidine.
Claim 26
Dependent Claim
14 words
The method of claim 16 wherein said second receptor is antibody for said antibodies.
Claim 27
Dependent Claim
11 words
The method of claim 16 wherein said label is an enzyme.
Claim 28
Dependent Claim
37 words
The method of claim 25 wherein a second enzyme is bound at said one or more situses to said strip, the enzymes being related in that the product of one enzyme is the substrate of the other.
Claim 29
Dependent Claim
23 words
The method of claim 16 wherein said situses are bands transverse to the direction of traversal of said test solution along said strip.
Claim 30
Dependent Claim
43 words
The method of claim 16 wherein the signal produced at said situses has a sharp-edged distinctive pattern that provides a sharp contrast to a signal produced at adjacent sites on said strip when one or more analytes are present in said test solution.
Claim 31
Dependent Claim
10 words
The method of claim 16 wherein said analytes are drugs.
References Cited (Backward Citations)
US4168146A
Cited
US4048298A
Cited
US3990850A
Cited
US4094647A
Cited
US4055394A
Cited
US4205058A
Cited
US4160008A
Cited
US4298685A
Cited
US4438067A
Cited
US4189304A
Cited
US4235601A
Cited
US4361537A
Cited
US4327073A
Cited
US4366241A
Cited
US4366241B1
Cited
US4384958A
Cited
US4434236A
Cited
WO1984002193A1
Cited
WO1984002397A1
Cited
US4459358A
Cited
EP0122032A2
Cited
EP0186799A1
Cited
EP0186779A2
Cited
US4740468A
Cited
EP0191640A2
Cited
US4806311A
Cited
EP0225054A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-09-24 | 1994-03-26 | 1994-09-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-09-24 | 1998-03-26 | 1998-09-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-09-24 | 2002-03-26 | 2002-09-25 | $7700.00 | Window Closed / Lapsed |